Abstract
Background: The pathogenesis of central serous chorioretinopathy is poorly understood. It is believed to be due to dysfunction of the retinal pigment epithelium and/or choroid and has been associated with elevated levels of epinephrine and administration of corticosteroids. Epinephrine and corticosteroids have previously been shown to induce apoptosis (programmed cell death) in various types of cells. The objective of this study was to investigate whether these agents can induce apoptosis in cultured retinal pigment epithelium cells. This may help elucidate the pathogenesis of central serous chorioretinopathy. Methods: Third-passage porcine retinal pigment epithelium cells were grown to confluence and incubated for 1–7 days in culture medium containing epinephrine (102–109 pg/ml) or a corticosteroid, dexamethasone (4–4×104 ng/ml). The cultures were evaluated for apoptosis by phase-contrast microscopy and in situ terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling. Results: Epinephrine (7×107– 109 pg/ml) induced apoptosis in a dose- and time-dependent manner. Exposure to lower concentrations of epinephrine (102–6×107 pg/ml) and all tested levels of dexamethasone did not result in apoptosis. Conclusion: Retinal pigment epithelium cells may undergo apoptosis following exposure to elevated levels of epinephrine. These findings suggest a possible pathophysiologic mechanism for the development of central serous chorioretinopathy.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 14 October 1999 Revised: 6 December 1999 Accepted: 7 December 1999
Rights and permissions
About this article
Cite this article
Sibayan, S., Kobuch, K., Spiegel, D. et al. Epinephrine, but not dexamethasone, induces apoptosis in retinal pigment epithelium cells in vitro: possible implications on the pathogenesis of central serous chorioretinopathy. Graefe's Arch Clin Exp Ophthalmol 238, 515–519 (2000). https://doi.org/10.1007/PL00007893
Issue Date:
DOI: https://doi.org/10.1007/PL00007893