Abstract
New cloning vectors were prepared with the aid of a large plasmid isolated fromAcetobacter pasteurianus and from plasmids pBR322 and pUC4-KAPA. Of the prepared cloning vectors, pACK5 contains a gene coding for kanamycin resistance, pACT7 and pACT71 contain a gene coding for tetracycline resistance and vector pACG3 with a gene coding for both kanamycin and tetracycline resistance. The vectors prepared only contained the beginning of replication from the pAC1 plasmid and possessed the ability to replicate withinE. coli andA. pasteurianus. The vectors are highly stable in both strains and during the 5-d cultivation under nonselective conditions are not eliminated.
Similar content being viewed by others
References
Barany F.: Single stranded hexameric linkers: a system for in-phase insertion mutagenesis and protein engineering.Gene 37, 111–119 (1985).
Birnboim H.C., Doly J.: A rapid alkaline extraction procedure for screening of recombinant plasmid DNA.Nucl. Acids Res. 7, 1513–1523 (1979).
Bolivar F., Rodriguez R.L., Greene P.J., Belach N.C., Heynecker H.L., Boyer H.W., Crosa J.H., Folkow S.: Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.Gene 2, 95–106 (1977).
Davis R.W., Botstein D., Roth J.R.:Advance Bacterial Genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 1980.
Grones J., Skerenova M., Bederkova K., Turňa J.: Isolation and characterization of plasmid pAC1 fromAcetobacter pasteurianus.Biologia (Bratislava) 44, 1191–1196 (1989).
Grones J., Skerenova M., Turna J.: Preparation of recombinant plasmids with kanamycin resistance in plasmid pAC1 fromAcetobacter pasteurianus.Biologia (Bratislava) 46, 673–678 (1991).
Fukaya M., Okumura T., Masai H., Uozumi T., Beppu T.: Construction of new shuttle vectors forAcetobacter.Agric. Biol. Chem. 49, 2083–2090 (1985).
Kleinschmidt A.K.: Monolayer techniques in electron microscopy of nucleic acid molecules.Methods Enzymol. 128, 361–377 (1968).
Low B.: Formation of merodiploids in matings with a class of Rec recipient strains ofEscherichia coli K12.Proc. Nat. Acad. Sci. USA 60, 160–168 (1968).
Mandel M., Higa A.: Calcium dendrit bacteriophage DNA infection.J. Mol. Biol. 53, 154–174 (1970).
Maniatis T., Fritsch E.F., Sambrook J.:Molecular Cloning—a Laboratory Manual. Cold Spring Harbor Laboratory, Gold Spring Harbor, New YOrk 1982.
Ohmori S., Uozoni T., Beppu T.: Loss of acetic acid resistance and ethanol oxidizing ability in anAcetobacter strain.Agric. Biol. Chem. 46, 381–389 (1982).
Okomura H., Uozumi T., Beppu T.: Construction of plasmid vectors and genetic transformation system forAcetobacter aceti.Agric. Biol. Chem. 49, 1011–1017 (1985).
Thomas P., Davis R.W.: Studies on the cleavage of bacteriophge lambda DNA withEcoRI restriction endunuclease.J. Mol. Biol. 91, 315–382 (1975).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Grones, J., Turňa, J. Construction of shuttle vectors for cloning inEscherichia coli andAcetobacter pasteurianus . Folia Microbiol 37, 395–400 (1992). https://doi.org/10.1007/BF02899895
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02899895