Abstract
Bacillus subtilis produces peptidolipid compounds of the iturin group that have been shown to have antifungal properties, but not all fungal species are sensitive to these compounds. In this study, the activity of iturin A, produced by B. subtilis strain B-3, was tested. Paper disks impregnated with various concentrations of iturin A were placed on agar plates seeded with conidia of toxigenic species of Fusarium, Gerlacia, Penicillium or Aspergillus. Most isolates were inhibited at iturin A concentrations as low as 4 μg/disk. Penicillium italicum, P. vindicatum, A. ochraceus and A. versicolor were most strongly inhibited by the iturin whereas P. citrinum and A. parasiticus were least sensitive to iturin A.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Delcambe L, Peypoux F, Guinand M, Michel G. Iturin and iturinic acids. Revue des Fermentations et des Industries Alimentares 1976; 31: 147–51.
Gueldner RC, Reilly CC, Pusey PL, Costello CE, Arrendale RF, Cox RH, Himmelsbach DS, Crumley FG, Cutler HG. Isolation and identification of iturins as antifungal peptides in biological control of peach brown rot with Bacillus subtilis. J Agric Food Chem 1988; 36: 366–70.
Pusey PL, Wilson CL. Postharvest control of stone fruit brown rot by Bacillus subtilis. Plant Disease 1984; 68: 753–56.
Pusey PL, Wilson CL. Postharvest biological control of stone fruit brown rot by Bacillus subtilis. 1988. US Patent number 4,764,371.
Mhammedi A, Peypoux F, Besson F, Michel G. Bacillomycin F, a new antibiotic of iturin group: isolation and characterization. J Antibiot 1982; 35: 306–11.
Winkelmann G, Allgaier H, Lupp R, Jung G. Iturin AL — A new long chain iturin A possessing an unusual high content of C16-Beta-Amino acids. J Antibiot 1983; 36: 1451–57.
Blocquiaux S, Delcambe L. Essais de traitement de dermatomycoses par l'iturine. Arch Belg Dermatol Syphiligr 1956; 12: 224–26.
Corry JL. The water relations and heat resistance of micro-organisms. Prog Ind Microbiol 1973; 12: 73–108.
Windels CE, Lindow SE, eds. Biological Control on the Phylloplane. St. Paul MN: American Phytopathological Society, 1985, 169pp.
McKeen CD, Reilly CC, Pusey PL. Production and partial characterization of antifungal substances antagonistic to Monilia fructicola from Bacillus subtilis. Phytopathology 1986; 76: 136–39.
Stewart MM, Young JD. Solid phase peptide synthesis, 2nd ed. Rockford IL: Pierce Chemical Company, 1984: 121.
Keen NT, Sims JJ, Erwin DC, Rice E, Partridge JE. 6a-hydroxyphaseollin: an antifungal chemical induced in soybean hypocotyls by Phytophthora megasperma var. sojae. Phytopathology 1971; 61: 1084–89.
SAS Institute Inc. SAS/STAT user's guide release 6.03. Cary NC: SAS Institute Inc, 1988: 623.
Isogai A, Takayama S, Murakoshi S, Suzuki A. Structures of beta amino acids in antibiotics iturin A. Tetrahedron Lett 1982; 23: 3065–68.
Anonymous. Alternative Agriculture. National Research Council. Washington DC: National Academy Press, 1989: 448 pp.
Latoud C, Peypoux F, Michel G, Genet R, Morgat JL. Interactions of antibiotics of the iturin group with human erythrocytes. Biochim Biophys Acta 1986; 856: 526–35.
Author information
Authors and Affiliations
Additional information
Mention of a trademark or proprietary product does not constitute a guarantee or warranty by the US Department of Agriculture and does not imply approval to the exclusion of other products that may also be suitable.
Rights and permissions
About this article
Cite this article
Klich, M.A., Lax, A.R. & Bland, J.M. Inhibition of some mycotoxigenic fungi by iturin A, a peptidolipid produced by Bacillus subtilis . Mycopathologia 116, 77–80 (1991). https://doi.org/10.1007/BF00436368
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00436368