Skip to main content
SpringerLink
Log in

A modified experimental set-up for sedimentation equilibrium experiments with gels. Part I: The instrumentation

  • Conference paper
  • First Online:
Ultracentrifugation

Part of the book series: Progress in Colloid & Polymer Science ((PROGCOLLOID,volume 94))

Abstract

An experimental set-up for the determination of swelling pressure-concentration curves and thermodynamic and elastic properties of gels by means of analytical ultracentrifugation is presented. The instrumentation described allows an accurate measurement of up to 70 samples simultaneously in an equilibrium experiment which lasts about 2 days if the 10-hole multichannel centerpieces described in part 2 of this trilogy are used. The number of samples is enough to characterize one cofmeplete gel/solvent system by important thermodynamic, structural and elastic parameters in the experimentally accessible range. It is of great advantage that all samples are measured under exactly the same conditions. Thisd is not possible by means of classical methods which all yield only one swelling pressure corresponding to one concentration. The evaluation process of the Schlieren patterns is fully automated to ensure a user-friendly process.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Tanaka T (1987) In: Mark, Bikales, Overberger, Menges (eds) Encyclopedia of Polymer Science and Engineering. Vol 7, John Wiley & Sons, p 514

    Google Scholar 

  2. Cölfen H (1991) Diplomarbeit, Duisburg

    Google Scholar 

  3. Holtus G, Cölfen H, Borchard W (1991) Progr Colloid Polym Sci 86:92

    Article  CAS  Google Scholar 

  4. Morchard W, Cölfen H (1992) Makromol Chem, Macromol Symp 61:143

    Google Scholar 

  5. Hinsken H (1992) Diplomarbeit, Duisburg

    Google Scholar 

  6. Hinsken H, Borchard W (1993) submitted

    Google Scholar 

  7. Cölfen H (1993) Dissertation, Duisburg

    Google Scholar 

  8. Holtus H (1990) Dissertation, Duisburg

    Google Scholar 

  9. Cölfen H, Borchard W (1991) Progr Colloid Polym Sci 86:102

    Google Scholar 

  10. Holtus G, Borchard W (1989) Colloid Polym Sci 267:1133

    Article  CAS  Google Scholar 

  11. Yphantis DA (1960) “Rapid determination of molecular weights of peptides and proteins” Ann N Y Acad Sci 88:586

    Article  CAS  Google Scholar 

  12. van Holde KE, Baldwin RL (1958) J Phys Chem 62:734

    Article  Google Scholar 

  13. Laue TM (1992) “Short Column Sedimentation Equilibrium Analysis for Rapid Characterization of Macromolecules in Solution” Beckman Instruments, Technical Information

    Google Scholar 

  14. Cölfen H, Borchard W, “A modified experimental set-up for sedimentation equilibrium experiments with gels: Part II Technical developments” (submitted to Anal Biochem)

    Google Scholar 

  15. Cölfen H, Borchard W, ”A modified experimental set-up for sedimentation equilibrium experiments with gels: Part III Results” (in preparation)

    Google Scholar 

  16. Borchard W (1991) Progr Colloid Polym Sci 86:84

    CAS  Google Scholar 

  17. Borchard W (1993) this issue

    Google Scholar 

  18. Svedberg T, Pedersen KO (1940) Die Ultrazentrifuge. Steinkopff, Dresden

    Google Scholar 

  19. Johnson P (1971) J Photograph Sci 19:49

    CAS  Google Scholar 

  20. Johnson P (1970) Velocity and equilibrium aspects of the sedimentation of agar gels in Photographic Gelatin I, Academic Press, London: 13

    Google Scholar 

  21. Bloomfield VA (1976) Biopolymers 15:1243

    Article  CAS  Google Scholar 

  22. Cölfen H, Borchard W (1993) submitted to Makromol Chem

    Google Scholar 

  23. Paul CH, Yphantis DA (1972) Anal Biochem 48:588

    Article  CAS  Google Scholar 

  24. Ortlepp B, Panke D (1991) Progr Colloid Polym Sci 86:57

    CAS  Google Scholar 

  25. Mächtle W, Klodwig U (1989) Colloid Polym Sci 267:1117

    Article  Google Scholar 

  26. Laue TM (1992) “On-line Data Aquisition and Analysis from the Rayleigh Interferometer” in Harding SE et al (Ed) “Analytical Ultracentrifugation in Biochemistry and Polymer Science” Royal Society of Chemistry, Cambridge, England: 63

    Google Scholar 

  27. Laue TM, “Real time interferometry in the ultracentrifuge” (1993) SPIE Proceedings Vol 1895 “Ultrasensitive Laboratory Diagnostics” (in press)

    Google Scholar 

  28. Holtus G, Kiepen F, Schwark K, Borchard W (1990) Makromol Chem, Rapid Commun 11:177

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Angewandte Physikalische Chemie, Universität-GH-Duisburg, Lotharstraße 1, 47048, Duisburg, FRG

    H. Cölfen & W. Borchard

Authors
  1. H. Cölfen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. W. Borchard
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

M. D. Lechner

Rights and permissions

Reprints and permissions

Copyright information

© 1994 Dr. Dietrich Steinkopff Verlag GmbH & Co. KG

About this paper

Cite this paper

Cölfen, H., Borchard, W. (1994). A modified experimental set-up for sedimentation equilibrium experiments with gels. Part I: The instrumentation. In: Lechner, M.D. (eds) Ultracentrifugation. Progress in Colloid & Polymer Science, vol 94. Steinkopff. https://doi.org/10.1007/BFb0115606

Download citation

  • DOI: https://doi.org/10.1007/BFb0115606

  • Received:

  • Accepted:

  • Published:

  • Publisher Name: Steinkopff

  • Print ISBN: 978-3-7985-0982-5

  • Online ISBN: 978-3-7985-1675-5

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation