Abstract
By using analytical ultracentrifugation interactions of the small heat shock protein hsp25 and G-actin have been analyzed. Association constants of about 107 reciprocal molar concentration were estimated from the concentration distribution using a computer program based on the nonlinear least-squares methode. In the presence of an excess of actin up to four molecules of this protein were bound to hsp25 particles.
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Lindquist S (1986) Ann Rev Biochem 55:1151–1191
Ingolia TD, Craig EA (1982) Proc Natl Acad Sci USA 70:2360–2364
Hickey E, Brandon SE, Potter R, Stein G, Stein J, Weber LA (1986) Nucleic Acid Res 14:4127–4145
DeJong WW, Leunissen JAM, Leenen PJM, Zweers A, Versteeg M (1988) J Biol Chem 263:5141–5149
Siezen RJ, Bindels JG, Hoenders HJ (1980) Eur J Biochem 111:435–444
Arrigo AP, Welch WJ (1987) J Biol Chem 262:15359–15369
Behlke J, Lutsch G, Gaestel M, Bielka H (1991) FEBS Lett 288:119–122
Miron T, Vancompernolle K, Vanderkerckhove J, Wilchek M, Geiger B (1991) J Cell Biol 114:255–261
Spudich JA, Watts S (1971) J Biol Chem 246:4866–4871
Pardel JD, Spudich JA (1982) Methods Enzymol 85:164–181
Behlke J, Knespel A, Glaser RW, Pleißner K-P (1991) Progr Colloid Polym Sci 86:3–35
Yphantis DA, Waugh DF (1956) J Phys Chem 60:630–636
Johnson LM, Correia JJ, Yphantis DA, Halvorson HR (1981) Biophys J 36:575–588
Levenberg K (1944) Quart Appl Math 2:164–172
Marquardt J (1963) Soc Indust Appl Math 11:431–441
Wynne CG, Wormell PMJH (1963) Applied Optics 2:1233–1238
Wyman J, Gill SJ (1990) Binding and Linkage, University Science Books Mill Valley, USA, 1990
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© 1994 Dr. Dietrich Steinkopff Verlag GmbH & Co. KG
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Behlke, J., Ristau, O., Knespel, A. (1994). Analysis of interaction of the small heat shock protein hsp25 with actin by analytical ultracentrifugation. In: Lechner, M.D. (eds) Ultracentrifugation. Progress in Colloid & Polymer Science, vol 94. Steinkopff. https://doi.org/10.1007/BFb0115600
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DOI: https://doi.org/10.1007/BFb0115600
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