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Fed-batch techniques in microbial processes

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Bioprocess Parameter Control

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 30))

Abstract

This article focuses on the recent world-wide advances of the biotechnology of the increasingly important fed-batch (or semi-batch) cultivation technique used in microbial processes. The history and characteristics of the fed-batch technique in microbial reactions are reviewed and examples of those fed-batch operations are cited which have greatly increased the productivity of microbial conversions in comparison to conventional batch operation; the various fed-batch techniques will be classified according to the mode of nutrient-feeding. Theoretical mathematical models are compared with experimental results. Fed-batch cultures with automatic feedback control are discussed in detail. Cultivation of high microbial cell concentrations, repeated fed-batch operations, and the start-up of microbial processes are described and, finally, some future prospects of fed-batch techniques in microbial processes are discussed.

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Abbreviations

a1, a2, a3 :

constants

b1, b2, b3 :

constants

c:

increment in culture volume per unit volumetric feed of substrate

C:

dimensionless c

D:

dilution rate, h−1

DO, [DO]:

dissolved oxygen concentration, mol l−1 or ppm

f:

feed rate, l h−1 or g h−1

F:

dimensionless f

i:

concentration of inhibitory metabolite

J:

objective function

ky, k iy , k oy :

cell yields based on growth-limiting substrate, i-species, and oxygen, respectively

ks, k os :

saturation constants of growth and of oxygen consumption, respectively, g l−1

kL a:

volumetric oxygen transfer coefficient, h−1

Ks :

dimensionless ks

Kp :

proportional band

m, mi :

maintenance coefficients, g g−1 h−1

M:

dimensionless m

OAR:

oxygen absorption rate, mol l−1 h−1

p:

metabolite concentration, mol l−1

\(q_{o_2 }\) :

specific oxygen consumption rate, h−1

qp :

specific production rate of metabolite, h−1

r:

volumetric rate of formation and consumption, mol l−1

rc, r ′c , r ″c , r ⃛'c :

cell mass productivity, g l−1 h−1

RQ:

respiratory quotient

s, si :

substrate concentration, g l−1

S:

dimensionless s

t:

time, h

T:

time constant

x:

cell concentration, g l−1

X:

dimensionless x

v:

volume of culture broth, l

V:

dimensionless v

z:

actuating signal

ε:

relative deviation

θ:

dimensionless time

θ:

time, h

\(\bar \Lambda\) :

mean age of culture

Μ:

specific growth rate, h−1

ϱ:

density, g cm−3

CO2 :

carbon dioxide

d:

differential

EtOH:

ethanol

est:

estimated

f:

final

i:

integral

in:

in the feed

max:

maximum

0:

initial

O2 :

oxygen

s:

mass or standard

T:

transition point

v:

volumetric

vap:

vaporization

w:

water

i:

i-species

max:

maximum

o:

oxygen dimensionless

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Authors and Affiliations

  1. Laboratory of Bioreaction Engineering, Department of Food Science and Technology, Faculty of Agriculture, Nagoya University, Chikusa-ku, 464, Nagoya, Japan

    Tsuneo Yamanè & Shoichi Shimizu

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  1. Tsuneo Yamanè
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  2. Shoichi Shimizu
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© 1984 Springer-Verlag

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Yamanè, T., Shimizu, S. (1984). Fed-batch techniques in microbial processes. In: Bioprocess Parameter Control. Advances in Biochemical Engineering/Biotechnology, vol 30. Springer, Berlin, Heidelberg. https://doi.org/10.1007/BFb0006382

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  • DOI: https://doi.org/10.1007/BFb0006382

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-13539-5

  • Online ISBN: 978-3-540-39004-6

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