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A Human Novel Gene DERPC Located on 16q22.1 Inhibits Prostate Tumor Cell Growth and Its Expression Is Decreased in Prostate and Renal Tumors

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Abstract

Background

Deletion of chromosome 16q is frequently associated with diverse tumors. Numerous studies strongly suggest the presence of one or more tumor suppressor genes on chromosome 16q22 to 16qter including the widely studied cadherin gene family. However, the specific tumor suppressor genes residing in this region need better definition and characterization.

Material and Methods

Standard molecular biology approaches have been used to clone and characterize the DERPC cDNA and its protein product on chromosome 16q22.1. Northern blotting was used to define the expression pattern in a multiple human tissue blots. DERPC expression was examined in multi-tumor array (Clontech, CA, USA) dot blot as well as in laser capture microdissection (LCM) derived prostate cancer (CaP) specimens by quantitative RT-PCR. Western blot analysis and a fluorescent microscopy were used to characterize the molecular size and the cellular location of green fluorescent protein (GFP)-tagged DERPC fusion proteins. A colony formation assay was conducted to determine the effects of DERPC expression on tumor cell growth.

Results

A novel gene DERPC (Decreased Expression in Renal and Prostate Cancer) was identified and characterized. DERPC encoded a strong basic, proline- and glycinerich nuclear protein. DERPC was ubiquitously expressed, with abundant expression in kidney, skeletal muscle, testis, liver, ovary, and heart and moderate expression in prostate. DERPC expression was reduced in renal (67%) and prostate tumors (33%). Expression of DERPC has inhibitory potential on CaP cell growth. Further, overexpression of DERPC in LNCaP cells caused alterations of nuclear morphology.

Conclusion

This study suggests that decreased expression of DERPC may be implicated in tumorigenesis of renal and CaPs.

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Acknowledgments

We thank Ms Justine Cowan for her critical reading of the manuscript. This work was supported by the Center for Prostate Disease Research, a program of the Henry M. Jackson Foundation for the Advancement of Military Medicine (Rockville, MD), funded by the United States Army Medical Research and Material Command.

The author is supported by a grant from the Center for Prostate Disease Research, a program of the Henry M. Jackson Foundation for the Advancement of Military Medicine (Rockville, MD), funded by the U.S. Army Medical Research and Materiel Command.

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Authors and Affiliations

  1. Center for Prostate Disease Research, Department of Surgery, Uniformed Services University of the Health Sciences, 1530 East Jefferson Street, Rockville, MD, 20852, USA

    Mei Sun, Lanfeng Ma, Linda Xu, Jia Li, Gyorgy Petrovics, Mazen Makarem, Judd Moul, Shiv Srivastava & Zhiqiang Zou

  2. Department of Genitourinary Pathology, Armed Forces Institute of Pathology, Washington, DC, USA

    Wei Zhang & Isabell Sesterhenn

  3. Section Lipid Cell Biology, Laboratory of Cell Biochemistry and Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health Bethesda, Bethesda, MD, USA

    Mei Zhang & E. Joan Blanchette-Mackie

  4. Urology Service, Department of Surgery, Walter Reed Army Medical Center, Washington, DC, USA

    Judd Moul

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  1. Mei Sun
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Correspondence to Zhiqiang Zou.

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The opinions and assertions contained herein are the private views of the authors and are not to be considered as reflecting the views of the U.S. Army or the Department of Defense.

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Sun, M., Ma, L., Xu, L. et al. A Human Novel Gene DERPC Located on 16q22.1 Inhibits Prostate Tumor Cell Growth and Its Expression Is Decreased in Prostate and Renal Tumors. Mol Med 8, 655–663 (2002). https://doi.org/10.1007/BF03402176

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  • DOI: https://doi.org/10.1007/BF03402176

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