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A Comparison of Ultrastructural Changes of Barley Cells Infected with Mild and Aggressive Isolates of Barley stripe mosaic virus

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Abstract

Electron microscopy studies were carried out to investigate the association of visible disease symptoms with ultrastructural changes in barley plants infected with mild and aggressive isolates of Barley stripe mosaic virus (BSMV). Differences in virus particle concentrations and cytopathological alterations in leaves were observed. The formation of membranous inclusions, abnormal dumbbell-shaped mitochondria and the deposition of membrane structures between the cell wall and plasma membrane were characteristic for aggressive isolates, whereas the rearrangement of the thylakoids and appearing electron transparent vacuoles in the plastid stroma were typical for the mild isolate. Moreover, the invaginations of plasma membrane into the cell and the cytoplasm containing a large number of virus particles into the vacuole, elongated mitochondria and alteration of micro-bodies were observed in the case of both isolates.

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References

  • Almási A, Apatini D & Bóka K, Böddi B & Gáborjányi R, 2000. BSMV infection inhibits chlorophyll biosynthesis in barley plants. Physiol Mol Plant Pathol 56, 227–233.

    Article  Google Scholar 

  • Barker H & Harrison BD, 1976. Infection of Tobacco mesophyll protoplasts with Raspberry ringspot virus alone and together with Tobacco rattle virus. J Gen Virol 35,125–133.

    Article  Google Scholar 

  • Bassi M, Appiano A, Barbieri N & D’Agosto G, 1985. Chloroplast alterations induces by Tomato bushy stunt virus in datura leaves. Protoplasma 126, 233–235.

    Article  Google Scholar 

  • Bragg JN & Jackson AO, 2004. Barley stripe mosaic. In Lapierre, H & Signoret, PA (Ed.) 2004: Viruses and Virus Diseases of Poaceae (Gramineae). INRA, Paris. 456–457.

    Google Scholar 

  • Brunt AA, Crabtree K, Dallwitz MJ, Gibbs AJ, Watson L & Zurcher EJ, 1996. Plant Viruses Online: Descriptions and Lists from the VIDE Database. Article first published online: 20 AUG 1996 | URL http://biology.anu.edu.au/Groups/MES/vide/.

    Google Scholar 

  • Carroll TW, 1969. Electron microscopic evidence for the presence of Barley stripe mosaic virus in cells of barley embryos. Virology 37, 649–657.

    Article  CAS  PubMed  Google Scholar 

  • Carroll TW, 1970. Relation of Barley stripe mosaic virus to plastids. Virology 42, 1015–1022.

    Article  CAS  PubMed  Google Scholar 

  • Carroll TW, 1974. Barley stripe mosaic virus in sperm and vegetative cells of barley pollen. Virology 60, 21–28.

    Article  CAS  PubMed  Google Scholar 

  • Carroll TW, 1986. Hordeiviruses, Biology and Pathology. In: Van Regenmortel, MHV & Fraenkel-Conrat, H (Ed.) 1986: The Plant Viruses Volume 2. The rod-shaped plant viruses. Plenum Press, New York and London. 373–395.

    Google Scholar 

  • Cass DD & Jensen WA, 1970. Fertilization in barley. Amer J Bot 57, 62–70.

    Article  Google Scholar 

  • Chong J & Haber S, 1992. Cytological alterations associated with flame chlorosis, a novel viruslike disease of barley, wheat, and oat. Phytopathology 82, 815–821.

    Article  Google Scholar 

  • Clark MF & Adams AN, 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J Gen Virol 34, 475–483.

    Article  CAS  PubMed  Google Scholar 

  • Francki RIB, Milne RG & Hatta T, 1984. Atlas of plant viruses, Volume II. CRC Press., Boca Raton, USA.

  • Gao R, Liu P & Wong S-M, 2012. Identification of plant viral RNA genome in the nucleus. Plos One 7, e48736.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gardner WS, 1967. Electron microscopy of Barley stripe mosaic virus: comparative cytology of tissues infected during different stages of maturity. Phytopathology 57, 1315–1326.

    Google Scholar 

  • Harsányi A, Böddi B & Bóka K, Almási A & Gáborjányi R, 2002. Abnormal etioplast development in barley seedlings infected with BSMV by seed transmission. Physiol Plantarum 114, 149–155.

    Article  Google Scholar 

  • Hibino H, Esuchizaki T & Saito Y, 1974. Comparative electron microscopy inclusions induced by 9 isolates of Soil-born wheat mosaic virus. Virology 57, 510–521.

    Article  CAS  PubMed  Google Scholar 

  • Hoefert LL, Esau K & Duffus JE, 1970. Electron microscopy of beta leaves infected with Beet yellow stunt virus. Virology 42, 814–824.

    Article  CAS  PubMed  Google Scholar 

  • Honda Y & Matsui C, 1971. Electron microscopy of intracellular Radish mosaic virus. Phytopathol 62, 448–452.

    Article  Google Scholar 

  • Jackson AO, Petty ITD, Jones RW, Edwards MC & French R, 1991. Analysis of barley stripe mosaic virus pathogenicity. Sem Virol 2, 107–119.

    CAS  Google Scholar 

  • Jeżewska M, 2001. Identification of Barley stripe mosaic virus in Poland. J Plant Prot Res 41, 164–167.

    Google Scholar 

  • Jeżewska M, 2006. Wirusy zbóz przenoszone przez nasiona-występowanie w Polsce i potencjalna szkodliwość. [Seedtransmitted cereal viruses-occurrence in Poland and their potential harmfulness]. Habilitation dissertation. Rozprawy Naukowe Instytutu Ochrony Roslin, Zeszyt 14. ISSN 1730-038X.

    Google Scholar 

  • Kendall A, Williams D, Bian W, Stewart PL & Stubbs G, 2013. Barley stripe mosaic virus: structure and relationship to the tobamoviruses. Virology 443, 265–270.

    Article  CAS  PubMed  Google Scholar 

  • Koenig R, An D, Lesemann De & Burgermeister W, 1987. Isolation of Carnation ringspot virus from canal near a sewage plant: cDNA hybridization analysis, serology and cytopathology. J Phytopathol 121, 346–356.

    Article  Google Scholar 

  • Lin NS & Langenberg WG, 2004. Chronology of appearance of Barley stripe mosaic virus protein infected wheat cells. J Ultra Res 89, 309–323.

    Article  Google Scholar 

  • Martelli G & Russo M, 1976. Unusual cytoplasmic inclusions induced by Watermelon mosaic virus. Virology 72, 352–362.

    Article  CAS  PubMed  Google Scholar 

  • McMullen CR, Gardner WS & Myers GA, 1977. Ultrastructure of cell wall thickenings and paramural bodies induced by Barley stripe mosaic virus. Phytopathology 67, 462–467.

    Article  Google Scholar 

  • McMullen CR, Gardner WS & Myers GA, 1978. Aberrant plastids in barley leaf tissue infected with Barley stripe mosaic virus. Phytopathology 68, 317–325.

    Article  Google Scholar 

  • Rana GL, Franco AD, Piazzolla P & Migliori A, 1987. Further studies on a Tomato ring virus isolate from Artichoke. J Phytopathol 118, 203–211.

    Article  Google Scholar 

  • Russo M & Martelli GP, 1972. Ultrastructural observations of Tomato bushy stunt virus in plant cells. Virology 49, 122–129.

    Article  CAS  PubMed  Google Scholar 

  • Shalla TA, 1959. Relations of Tobacco mosaic virus and Barley stripe mosaic virus to their host cells as revealed by ultrathin tissue-sectioning for the electron microscope. Virology 7, 193–219.

    Article  CAS  PubMed  Google Scholar 

  • Stewart AD, Logsdon JM & Kelley ST, 2005. An empirical study of the evolution of virulence under both horizontal and vertical transmission. Evolution 59, 730–73.

    Article  PubMed  Google Scholar 

  • Torrance L, Cowan GH, Gillespie T, Ziegler A & Lacomme C, 2006. Barley stripe mosaic virus-encoded proteins triple-gene block 2 and γb localize to chloroplasts in virus-infected monocot and dicot plants, revealing hitherto-unknown roles in virus replication. J Gen Virol 87, 2403–2411.

    Article  CAS  PubMed  Google Scholar 

  • Weintraub M & Ragetli HWJ, 1970. Electron microscopy of the bean and cowpea strains of Southern bean mosaic virus within leaf cells. J Ultra Res 32, 167–189.

    Article  CAS  Google Scholar 

  • Zarzyńska A, Jeżewska M, Trzmiel K & Hasiów-Jaroszerwska B, 2014. Development of a one-step immunocapture realtime RT-PCR assay for the detection of Barley stripe mosaic virus strains in barley seedlings. Acta Virol 58, 81–85.

    Article  PubMed  Google Scholar 

  • Zielińska L & Pospieszny H, 2002. Ultrastructural changes in leaf cells infected with Arabis mosaic nepovirus II. in Chenopodium quinoa plants. J Plant Protect Res 42, 189–198.

    Google Scholar 

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Authors and Affiliations

  1. Department of Virology and Bacteriology, Institute of Plant Protection-National Research Institute, Wł. Węgorka 20, 60-318, Poznań, Poland

    Aleksandra Zarzyńska-Nowak, Małgorzata Jeżewska, Beata Hasiów-Jaroszewska & Lidia Zielińska

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  1. Aleksandra Zarzyńska-Nowak
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  2. Małgorzata Jeżewska
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  3. Beata Hasiów-Jaroszewska
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  4. Lidia Zielińska
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Correspondence to Aleksandra Zarzyńska-Nowak.

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Zarzyńska-Nowak, A., Jeżewska, M., Hasiów-Jaroszewska, B. et al. A Comparison of Ultrastructural Changes of Barley Cells Infected with Mild and Aggressive Isolates of Barley stripe mosaic virus. J Plant Dis Prot 122, 153–160 (2015). https://doi.org/10.1007/BF03356545

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  • DOI: https://doi.org/10.1007/BF03356545

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