References
Beresford JN, Gallagher JA, Gowen M, McGuire MKB, Poser J, Russell RGG (1983) Human bone cells in culture. A novel system for the investigation of bone cell metabolism. Clin Sci 64:38–39
Beresford JN, Gallagher JA, Russell RGG (1986) 1,25-Dihydroxyvitamin D3 and human bone-derived cells in vitro: effects on alkaline phosphatase, type 1 collagen and proliferation. Endocrinology 119:1776–1785
Beresford JN (1984) Studies on the metabolism of human bone cells in vitro. II. Ph.D. Thesis, University of Sheffield, UK
Beresford JN, Gallagher JA, Poser JW, Russell RGG (1984) Production of osteocalcin by human bone cells in vitro. Effects of 1,25-(OH)2D3, 24,25-(OH)2D3, parathyroid hormone and glucocorticoids. Metab Bone Dis Rel Res 5:229–234
Beresford JN, Graves SE, Smoothy CA (1993) Formation of mineralised nodules by bone-derived cells in vitro: a model of bone formation? Am J Med Genet 45:163–178
Meikle MC, Bord S, Hembry RM, Compston J, Croucher PI, Reynolds JJ (1992) Human osteoblasts in culture synthesize collagenase and other matrix metalloproteinases in response to osteotropic hormones and cytokines. J Cell Science 103:1093–1099
Birch MA, Ginty AF, Walsh CA, Fraser WD, Gallagher JA, Bilbe G (1993) PCR detection of cytokines in normal human and pagetic osteoblast-like cells. J Bone Miner Res 8:1155–1162
Ashton BA, Abdullah F, Cavé J, Williamson M, Sykes BC, Couch M, Poser JW (1985) Characterization of cells with high alkaline phosphatase activity derived from human bone and marrow: preliminary assessment of their osteogenicity. Bone 6:313–319
Gundle R, Bradley J, Joyner C, Francis M, Triffitt J, Beresford J (1993) Bone formation in vitro and in vivo by cultured adult human bone-derived cells. J Bone Miner Res 8(suppl 1):S374
Ashton BA, Eaglesom CC, Bab I, Owen ME (1984) Distribution of fibroblastic colony-forming cells in rabbit bone marrow and assay of their osteogenic potential by an in vivo diffusion chamber method. Calcif Tissue Int 36:83–86
Beresford JN (1989) Osteogenic stem cells and the stromal system of bone and marrow. Clin Orthop Rel Res 240:270–280
Cheng SL, Yang JW, Rifas L, Zhang SF, Avioli LV (1994) Differentiation of human bone marrow osteogenic stromal cells in vitro: induction of the osteoblast phenotype by dexamethasone. Endocrinology 134:277–286
Gundle R, Joyner C, Bradley J, Francis M, Triffitt J (1994) Bone formation in vivo by cultured human marrow stromal and trabecular bone-derived cells. (abstract) Bone 15:230
Silve C, Grosse B, Tau C, Garabedian M, Fritsch J, Delmas PD, Cournot-Witmer G, Balsan S (1986) Response to parathyroid hormone and 1,25-dihydroxyvitamin D3 of bone-derived cells isolated from normal children and children with abnormalities in skeletal development. J Clin Endocrinol Metab 62:583–590
Brenner RE, Nerlich A, Kirchner F, Morike M, Terinde R, Teller WM (1993) Proliferation and collagen biosynthesis of osteoblasts and chondrocytes in short rib syndrome type beemer. Am J Med Genet 46:584–591
Morike M, Schulz M, Brenner RE, Bushart GB, Teller WM, Vetter U (1993) In vitro expression of osteoblast markers in cells isolated from normal fetal and postnatal human bone and from bone of patients with osteogenesis imperfecta. J Cell Physiol 157:439–444
Puzas JE, Brand JS, Jackman KV (1986) Isolation and characterisation of bone cells from a patient with osteogenesis imperfecta type Ib. Bone Miner 1:375–382
Liberman UA, Eil C, Holst P, Rosen JF, Marx SJ (1983) Hereditary resistance to 1,25-dihydroxy vitamin D: defective function of the receptors for 1,25-dihydroxy vitamin D in cells cultured from bone. J Clin Endocrinol Metab 57:958–962
Compston JE, Croucher PI (1991) Histomorphometric assessment of trabecular bone remodelling in osteoporosis. Bone Miner 14:91–102
Parfitt AM (1990) Bone-forming cells in clinical conditions. In: Hall BK (ed) Bone, a treatise, vol 1: the osteoblast and osteocyte. The Telford Press, Caldwell, NJ, pp 351–429
Cohen-Solal ME, Shih M-S, Lundy MW, Parfitt AM (1991) A new method for measuring cancellous bone erosion depth: application to the cellular mechanisms of bone loss in postmenopausal osteoporosis. J Bone Miner Res 6:1331–1338
Parfitt AM, Shih M-S, Rao DS, Kleerekoper M (1991) Relation between bone formation rate and osteoblast surface in ageing and osteoporosis: evidence for impaired osteoblast recruitment in pathogenesis (suppl) J Bone Miner Res (7) 1:S116
Owen ME, Friedenstein AJ (1988) Stromal stem cells: marrow-derived osteogenic precursors. In: Cell and molecular biology of vertebrate hard tissues. Ciba Foundation Symposium 136. John Wiley & Sons Ltd., Chichester, UK pp 42–60
Bennett JH, Joyner CJ, Triffitt JT, Owen ME (1991) Adipocytic cells cultured from marrow have osteogenic potential. J Cell Science 99:131–139
Meunier P, Aaron J, Edouard C, Vignon G (1971) Osteoporosis and the replacement of cell populations of the marrow by adipose tissue. Clin Orthop Rel Res 80:147–154
Burkhardt R, Kettner G, Bohm W, Schmidmeir M, Schlag R, Frisch B, Mailman B, Eisenmenger W, Gilg TH (1987) Changes in trabecular bone, haematopoiesis and bone marrow vessels in aplastic anaemia, primary osteoporosis and old age: a comparative and histomorphometric study. Bone 8:157–164
Gundle R, Graves SE, Francis MJO, Beresford JN (1994) Osteogenic and adipogenic differentiation in human bone-derived cell cultures (abstract) Bone 15:114–115
Beresford JN, Bennett JH, Devlin C, Leboy PS, Owen ME (1992) Evidence for an inverse relationship between the differentiation of adipocytic and osteogenic cells in rat marrow stromal cell cultures. J Cell Sci 102:341–351
Dorheim M-A, Sullivan M, Dandapani V, Wu X, Hudson J, Segarini PR, Rosen DM, Aulthouse AL, Gimble JM (1993) Osteoblastic gene expression during adipogenesis in haematopoietic supporting murine bone marrow stromal cells. J Cell Physiol 154:317–328
Brenner RE, Riemenschneider B, Blum W, Morike M, Teller WM, Pirsig W, Heinze E (1992) Defective stimulation of proliferation and collagen biosynthesis of human bone cells by serum from diabetic patients. Acta Endocrinol 127:509–514
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gundle, R., Beresford, J.N. The Isolation and Culture of Cells from Explants of Human Trabecular Bone. Calcif Tissue Int 56 (Suppl 1), S8–S10 (1995). https://doi.org/10.1007/BF03354640
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF03354640