Abstract
In several cases of thyroid adenoma in which no TSH was detectable in the serum, a paradoxical decrease of PB123I and its conversion rate Q (PB123I / serum total radioactivity) was observed after infusion of protirelin (TRH). The possible direct effect of TRH on thyrocytes was therefore studied in vitro. Porcine thyroid cells were cultured in NCTC-135 medium supplemented with a serum substitute and insulin. TRH and some of its analogs enhanced the incorporation of [3H] thymidine into DNA and the uptake of radioiodide into thyrocytes. In the presence of TSH, TRH did not stimulate ra-dioiodine uptake, whereas incorporation of [3H] thymidine into DNA was not antagonized by TSH. Thus, in this culture system, TRH had a direct effect on both growth and metabolism of thyrocytes. TSH can modulate these effects.
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Wahl, R., Brossart, P., Eizenberger, D. et al. Direct effects of protirelin (TRH) on cultured porcine thyrocytes. J Endocrinol Invest 15, 345–351 (1992). https://doi.org/10.1007/BF03348750
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DOI: https://doi.org/10.1007/BF03348750