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Serum concentrations of selected endogenous estrogen and estrogen metabolites in pre- and post-menopausal Chinese women with osteoarthritis

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Abstract

The purpose of this study was to investigate whether serum levels of selected endogenous estrogens and their metabolites are involved in the pathogenesis of osteoarthritis in pre- and post-menopausal women with osteoarthritis. Sixty-four patients with osteoarthritis (OA) of the knee, 48 patients with rheumatoid arthritis (RA) of the knee, and 48 healthy women were included in this study. Serum concentrations of estradiol and estrogen metabolites, such as 2-hydroxyestrone, 2-hydroxyestradiol, and 16α-hydroxyestrone, were measured by high performance liquid chromatography-mass spectrometry. Our results show that the serum concentrations of free estradiol and total 2-hydroxyestrone were significantly lower in pre-menopausal women with OA compared to the levels detected in the control groups (RA and healthy women). While serum concentrations of free and total estradiol in post-menopausal women with OA was significantly decreased compared to those of the control groups, the level of total 2-hydroxyestradiol significantly increased in postmenopausal women. Furthermore, the total 2-hydroxyestrone concentration positively correlated with the total estradiol level in pre-menopausal women with OA. In addition, the total 2-hydroxyestradiol level positively correlated with free and total estradiol levels in post-menopausal women with OA. In conclusion, estradiol and estrogen metabolites, including 2-hydroxyestrone and 2-hydroxyestradiol, were found in the sera of pre- and post-menopausal women with OA. Except for free and total estradiol deficiency, a decreased serum level of total 2-hydroxyestrone in pre-menopausal women and an increased total 2-hydroxyestradiol level in post-menopausal women with OA may also correlate with the pathogenesis of female OA.

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Authors and Affiliations

  1. Department of Orthopedics, the Third Affiliated Hospital of Sun Yat-sen University, Tian He District, No.600, Tianhe Road, Guangzhou, Guangdong, 510630, PR China

    W. Gao, C. Zeng, D. Cai, B. Liu, X. Wen & Y. Chen

  2. Department of Orthopedics, Linyi People’s Hospital, Linyi, Shandong

    W. Gao

  3. Department of Biological Products Detection, the Institute for the Control of Pharmaceutical and Biological Products, Guangzhou, Guangdong, PR China

    Y. Li

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  1. W. Gao
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  2. C. Zeng
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  3. D. Cai
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  4. B. Liu
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  5. Y. Li
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  6. X. Wen
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  7. Y. Chen
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Correspondence to D. Cai.

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W. Gao and C. Zeng comtributed equally to this work.

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Gao, W., Zeng, C., Cai, D. et al. Serum concentrations of selected endogenous estrogen and estrogen metabolites in pre- and post-menopausal Chinese women with osteoarthritis. J Endocrinol Invest 33, 644–649 (2010). https://doi.org/10.1007/BF03346664

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