Skip to main content
SpringerLink
Log in

Effect of hypothyroidism on synaptosomal-associated protein of 25 kDa and syntaxin-1 expression in adenohypophyses of rat

  • Original Article
  • Published:
Journal of Endocrinological Investigation Aims and scope Submit manuscript

Abstract

The aim of this study was to analyze the expression of SNAP-25 and syntaxin-1 in the adenohypophyses of hypothyroid rats. Rats were divided into: 1) controls; 2) thyroidectomized 40 days; and 3) thyroidectomized 40 days with replacement of T4 20 days after surgery. Adenohypophyses were studied by immunohistochemistry and immunoblot analysis using antibodies against SNAP-25, syntaxin-1 and TSH. By immunostaining, SNAP-25 and syntaxin-1 were conspicuous and were localized around cytoplasmic vacuoles of thyroidectomy cells. Immunoblot analysis shows that thyroidectomy increases adenohypophysial SNAP-25 expression and decreases syntaxin-1 levels. T4 administration for 20 days produces a recovery similar to control values. In conclusion, thyroidectomy produces changes in both expression and immunoreactivity of SNAP-25 and syntaxin-1 in adenohypophyses of rats and these effects can be reversed by T4 administration.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Beck-Peccoz P., Brucker-Davis F., Persani L., Smallridge R., Weintraub B.D. Thyrotropin-secreting pituitary tumors. Endocrin. Rev. 1996, 17: 610–638.

    CAS  Google Scholar 

  2. Asa S.L. Tumors of the pituitary gland. In: Atlas of tumor pathology. 3rd series, fascicle 22. Washington, D.C., Armed Forces Institute of Pathology, 194, 1998.

  3. Salinas E., Quintanar J.L. Neurofilaments in thyrotrophs of hypothyroid rats. An immunohistochemical study. Endocrin. Pathol. 2000, 11: 195–200.

    Article  Google Scholar 

  4. Horvath E., Lloyd R.V., Kovacs K. Propylthiouracyl-induced hypothyroidism results in reversible transdifferentiation os somatotrophs into thyroidectomy cell. Lab. Invest. 1990, 63: 511–520.

    PubMed  CAS  Google Scholar 

  5. Quintanar-Stephano A., Valverde- R C., Kovacs K. Mitotic count in rat adenohypophysial thyrotrophs and somatotrophs: effects of short-term thyroidectomy, thyroxine, and thyrotropin-releasing hormone. Endocrin. Pathol. 1999, 10: 335–341.

    Article  CAS  Google Scholar 

  6. Vidal S., Horvath E., Kovacs K., Cohen S.M., Lloyd R.V., Scheithauer B.W. Transdifferentiation of somatotrophs to thyrotrophs in the pituitary of patients with protracted primary hypothyroidism. Virchows Arch. 2000, 436: 43–51.

    Article  PubMed  CAS  Google Scholar 

  7. Stojilkovic S.S., Catt K.J. Calcium oscillation in anterior pituitary cells. Endocrin. Rev. 1992, 13: 256–280.

    Article  CAS  Google Scholar 

  8. Kwiecien R., Hammond C. Differential management of Ca2+ oscillations by anterior pituitary cells: A comparative overview. Neuroendocrinology 1998, 68: 135–151.

    Article  PubMed  CAS  Google Scholar 

  9. Jacobsson G., Meister B. Molecular components of the exocytosis machinery in the rat pituitary gland. Endocrinology 1996, 137: 5344–5356.

    PubMed  CAS  Google Scholar 

  10. Majó G., Aguado F., Blasi J., Marsal J. Synaptobrevin isoforms in secretory granules and synaptic-like microvesicles in anterior pituitary cells. Life Sci. 1998, 62: 607–616.

    Article  PubMed  Google Scholar 

  11. Kolk S.M., Nordquist R., Tuinhof R., Gagliardini L., Thompson B., Cool Roubous E.W. Localization and physiological regulation of the exocytosis protein SNAP-25 in the brain and pituitary gland of Xenopus laevis. J. Neuro-endocrinol. 2000, 12: 694–706.

    CAS  Google Scholar 

  12. Aguado F., Gombau L., Majó G., Marsal J., Blanco J., Blasi J. Regulated secretion is impaired in AtT-20 endocrine cells stably transfected with botulinum neurotoxin type A light chain. J. Biol. Chem. 1997, 272: 26005–26008.

    Article  PubMed  CAS  Google Scholar 

  13. Masumoto N., Ikebuchi Y., Matsuoka T., Tasaka K., Miyake A., Murata Y. Involvement of SNAP-25 in TRH-induced exocytosis in pituitary GH4C1 cells. J. Endocrinol. 1997, 153: R5–R10.

    Article  PubMed  CAS  Google Scholar 

  14. Majó G., Lorenzo M. J., Blasi J., Aguado F. Exocytotic protein components in rat pituitary gland after long-term estrogen administration. J. Endocrinol. 1999, 161: 323–331.

    Article  PubMed  Google Scholar 

  15. Lee M.S., Zhu Y.L., Sun Z., et al. Accumulation of synaptosomal-associated protein of 25 kDa (SNAP-25) and other proteins associated with the secretory pathway in GH4C1 cells upon treatment with estradiol, insulin and epidermal growth factor. Endocrinology 2000, 141: 3485–3492. peroxidase complex in immunoperoxidase technics. Am. J. Clin. Pathol. 1981, 75: 816.

    PubMed  CAS  Google Scholar 

  16. Hsu S.M., Raine L., Fanger H. The use of antiavidin antibody and avidin-biotin peroxidase complex in immunoperoxidase technics. Am. J. Clin. 1981, 75: 816.

    CAS  Google Scholar 

  17. Woosley J.T. Measuring cell proliferation. Arch. Pathol. Lab. Med. 1991, 115: 555–557.

    PubMed  CAS  Google Scholar 

  18. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72: 248–54.

    Article  PubMed  CAS  Google Scholar 

  19. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 1970, 227: 680–685.

    Article  PubMed  CAS  Google Scholar 

  20. Towbin H., Staehelin J., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. Proc. Natl. Acad. Sci. USA 1979, 76: 4350–4354.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  21. Majó G., Ferrer I., Marsal J., Blasi J., Aguado F. Immunocytochemical analysis of the synaptic proteins SNAP-25 and Rab3A in human pituitary adenomas. Overexpression of SNAP-25 in the mammosomatotroph lineages. J. Pathol. 1997, 183: 440–446.

    Article  PubMed  Google Scholar 

  22. Salinas E., Quintanar J.L., Reig J.A. Immunohistochemical study of syntaxin-1 and SNAP-25 in the pituitaries of mouse, guinea pig and cat. Acta Physiol. Pharmacol. Ther. Latinoam. 1999, 49: 61–64.

    PubMed  CAS  Google Scholar 

  23. Hepp R., Grant N.J., Espliguero G., et al. Adrenal gland SNAP-25 expression is altered in thyroid hormone receptor knock-out mice. Neuroreport 2001, 12: 1427–1430.

    Article  PubMed  CAS  Google Scholar 

  24. Jacobsson G., Razani H., Ogren S.O., Meister B. Estrogen down-regulates mRNA encoding the exocytotic protein SNAP-25 in the rat pituitary gland. J. Neuroendocrinol. 1998, 10: 157–163.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Neurophysiology, Department of Physiology and Pharmacology, Universidad Autónoma de Aguascalientes, Av. Universidad 940, C.P. 20100, Aguascalientes Ags, México

    J. L. Quintanar

  2. Laboratory of Immunology, Department of Microbiology, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes, México

    E. Salinas

Authors
  1. J. L. Quintanar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. Salinas
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. L. Quintanar.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Quintanar, J.L., Salinas, E. Effect of hypothyroidism on synaptosomal-associated protein of 25 kDa and syntaxin-1 expression in adenohypophyses of rat. J Endocrinol Invest 25, 754–758 (2002). https://doi.org/10.1007/BF03345507

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03345507

Key-words

Search

Navigation