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IgA Antibodies against Klebsiella and Other Gram-Negative Bacteria in Ankylosing Spondylitis and Acute Anterior Uveitis

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Summary

Mucosal infections, especially of the gastrointestinal tract, are thought to trigger the onset and/or reactivation of ankylosing spondylitis (AS). Previous investigations into the role of Klebsiella and other Gram- negative bacteria in AS patients show contrasting results. In the present study prevalence of IgA antibodies against Klebsiella, Yersinia, Salmonella, Shigella, and Campylobacter was examined in serum samples from 30 patients having HLA- B27 associated ankylosing spondylitis, 32 patients with HLA- B27 associated acute anterior uveitis AAU), and 27 HLA- B27 positive patients having both AS and AAU. Numbers of antibodies were compared with those in sera from 29 HLA- B27 negative patients with AAU, 26 healthy HLA- B27 positive and 31 HLA- B27 negative controls. IgA antibodies were detected using an indirect immunofluorescence assay on whole bacteria. In case of Yersinia, Salmonella, Shigella and Campylobacter, reference strains were used. Examination for anti- Klebsiella antibodies was performed using three different strains, isolated from patients with ankylosing spondylitis. The sera were tested on antibodies against Klebsiella K43 (BTS1) as well.

The number of IgA positive sera against Yersinia, Salmonella, Shigella, Campylobacter and Klebsiella K43 BTS1) did not differ between HLA- B27 positive patients and controls, nor among the various groups. Differences were neither observed when the Klebsiella strains from AS patients had been used as antigen.

These results do not confirm a relationship between HLA-B27 associated AS or AAU and infection with Klebsiella or other Gram- negative bacteria.

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Reference

  1. Keat, A. Reiter’s syndrome and reactive arthritis in perspective. N Engl J Med 1983, 309,1606–1615.

    Article  CAS  PubMed  Google Scholar 

  2. Ebringer, R., Cooke, D., Cawdell, D.R., Cowling, P., Ebringer, A. Ankylosing spondylitis: Klebsiella and HLA-B27. Rheumatology Rehab 1977, 16,190–196.

    Article  CAS  Google Scholar 

  3. Ebringer, R., Cawdell, D., Ebringer, A. Association of Klebsiella pneumoniae with acute anterior uveitis in ankylosing spondylitis. Br Med J 1979, 1, 383.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  4. Van Kregten, E., Huber-Bruning, O., Vandenbroucke, J.R, Willers, J.M.N. No conclusive evidence of an epidemiological relation between Klebsiella and ankylosing spondylitis. J Rheumatol 1991, 18, 384–388.

    PubMed  Google Scholar 

  5. Warren, R.E., Brewerton, D.A.. Faecal carriage of Klebsiella by patients with ankylosing spondylitis and rheumatoid arthritis. Ann Rheum Dis 1978, 37,146–151.

    Article  Google Scholar 

  6. Eastmond, C.J., Willshaw, H.E., Burgess, S.E.R, Shinebaum, R., Cooke, E.M., Wright, V. Frequency of faecal Klebsiella aerogenes in patients with ankylosing spondylitis and controls with respect to individual features of the disease. Ann Rheum Dis 1980, 39, 118–123.

    Article  CAS  PubMed  Google Scholar 

  7. Trull, A.K., Ebringer, R., Panayi, G.S., Colthorpe, D., James, D.C.O., Ebringer, A. IgA antibodies to Klebsiella pneumoniae in ankylosing spondylitis. Scand J Rheumatol 1983,12, 249–253.

    Article  CAS  PubMed  Google Scholar 

  8. Shodjai-Moradi, R, Ebringer, A., Abduljadayel, I. IgA antibody response to Klebsiella in ankylosing spondylitis measured by immunoblotting. Ann Rheum Dis 1992, 51, 233–237.

    Article  CAS  PubMed  Google Scholar 

  9. Mäki-Ikola, O., Lehtinen, K., Granfors, K., Vainionpää, R., Toivanen, R Bacterial antibodies in ankylosing spondylitis. Clin Exp Immunol 1991, 84, 472–475.

    PubMed Central  PubMed  Google Scholar 

  10. Sprenkels, S.H.D., Uksila, J., Toivanen, P., Feltkamp, T.E.W IgA antibodies in HLA-B27 associated acute anterior uveitis and ankylosing spondylitis. Submitted for publication.

  11. Van Bohemen, C.G., Nabbe, A.J.J.M., Goeithe, H.S., Dekker-Saeys, A. J., Zanen, H.C. Antibodies to Enterobacteriaceae in ankylosing spondylitis. Scand J Rheumatol 1986,15,143–147.

    Article  PubMed  Google Scholar 

  12. Van der Linden, S., Valkenburg, H.A., Cats, A. Evaluation of diagnostic criteria for ankylosing spondylitis. Arthritis Rheum 1984, 27, 361–368.

    Article  PubMed  Google Scholar 

  13. O’Connor, G.R. Current Classification of Uveitis. In: ed]Saari KM. Uveitis Update. Asterdam: Elsevier Science Publishers B.V. 1984, 3–6.

    Google Scholar 

  14. Van Kregten, E., Westerdaal, N.A.C., Willers, J.M.N. New simple medium for selective recovery of Klebsiella pneumoniae and Klebsiella oxytoca from human faeces. J Clin Microbiol 1984, 20, 936–941.

    PubMed Central  PubMed  Google Scholar 

  15. Sprenkels, S.H.D., Hoogkamp-Korstanje, J.A.A., Feltkamp, T.E.W. Antibodies against Yersinia “outer proteins” in HLA-B27 associated acute anterior uveitis and ankylosing spondylitis. Submitted for publication.

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Authors and Affiliations

  1. Department of Ophthalmo-Immunology, the Netherlands Ophthalmic Research Institute, 12141, 1100 AC, Amsterdam, the Netherlands

    S. H. D. Sprenkels & T. E. W. Feltkamp

  2. Eemland Hospital, Amersfoort, the Netherlands

    E. Van Kregten

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  1. S. H. D. Sprenkels
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  2. E. Van Kregten
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  3. T. E. W. Feltkamp
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Sprenkels, S.H.D., Van Kregten, E. & Feltkamp, T.E.W. IgA Antibodies against Klebsiella and Other Gram-Negative Bacteria in Ankylosing Spondylitis and Acute Anterior Uveitis. Clin Rheumatol 15 (Suppl 1), 48–51 (1996). https://doi.org/10.1007/BF03342646

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