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Biodegradation of polycyclic aromatic hydrocarbon by a halotolerant bacterial consortium isolated from marine environment

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Abstract

The biodegradability of polycyclic aromatic hydrocarbons such as naphthalene, fluorene, anthracene and phenanthrene by a halotolerant bacterial consortium isolated from marine environment was investigated. The polycyclic aromatic hydrocarbons degrading bacterial consortium was enriched from mixture saline water samples collected from Chennai (Port of Chennai, salt pan), India. The consortium potently degraded polycyclic aromatic hydrocarbons (> 95%) at 30g/L of sodium chloride concentration in 4 days. The consortium was able to degrade 39 to 45% of different polycyclic hydrocarbons at 60 g/L NaCl concentration. Due to increase in salinity, the percent degradation decreased. To enhance polycyclic aromatic hydrocarbons degradation, yeast extract was added as an additional substrate at 60g/L NaCl concentration. After the addition of yeast extract, the consortium degraded > 74 % of polycyclic aromatic hydrocarbons at 60 g/L NaCl concentration in 4 days. The consortium was also able to degrade PAHs at different concentrations (5, 10, 20, 50 and 100 ppm) with 30 g/L of NaCl concentration. The polycyclic aromatic hydrocarbons degrading halotolerant bacterial consortium consists of three bacterial strains, namely Ochrobactrum sp., Enterobacter cloacae and Stenotrophomonas maltophilia.

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Authors and Affiliations

  1. Department of Civil and Environmental Engineering, Sung Kyun Kwan University, 300 CheonCheon-Dong, Jangan-Gu, Suwon, Gyeonggi-Do, South Korea

    P. Arulazhagan Ph.D. (Post doctoral researcher) & I. T. Yeom Ph.D. (Professor)

  2. Centre for Environmental Studies, Anna University, Chennai, 600 025, India

    N. Vasudevan Ph.D. (Professor)

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  1. P. Arulazhagan Ph.D.
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  2. N. Vasudevan Ph.D.
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  3. I. T. Yeom Ph.D.
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Correspondence to P. Arulazhagan Ph.D..

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Arulazhagan, P., Vasudevan, N. & Yeom, I.T. Biodegradation of polycyclic aromatic hydrocarbon by a halotolerant bacterial consortium isolated from marine environment. Int. J. Environ. Sci. Technol. 7, 639–652 (2010). https://doi.org/10.1007/BF03326174

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  • DOI: https://doi.org/10.1007/BF03326174

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