Skip to main content
SpringerLink
Log in

High Frequency Clonal Propagation of Cymbopogon martinii var motia (palmarosa) Through Rhizome Culture and True to Type Assessment using ISSR Marker

  • Short Communication
  • Published:
Journal of Plant Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

A rapid and high frequency plant propagation system has been established in Cymbopogon martinii var motia using rhizome culture. Different concentrations of auxins, such as 5.36 μM Napthalene acetic acid (NAA) and 5.71μM Indole 3acetic acid (IAA) satisfactorily induced shoot buds when applied individually or in combination with 4.40μM N6-Benzyladenine (BA) on MS medium. Multiple shoot proliferation was noted when induced microshoots were cultured on MS medium supplemented with BA either alone or with NAA. Shoot bud induction and multiple shoot formation were enhanced significantly with the application of growth additives like coconut water (CW) and biotin. Out of the two auxins (IAA and IBA) tested, rooting percentage and number of roots/shoot was significantly higher on 1/2 MS medium supplemented with 4.90 μM Indole 3- butyric acid (IBA). Twelve reproducible ISSR primers efficiently screened 16 randomly chosen regenerants of C. martinii with similar monomorphic banding profiles exhibiting genetic stability of the regenerants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Abbreviations

NAA:

Napthaleneacetic acid

IAA:

Indole-3-acetic acid

IBA:

Indole 3-butyric acid

BA:

N6-Benzyladenine

Kn:

Kinetin

CW:

Coconut water

MS:

Murashige and Skoog Medium

References

  1. Nayak S, Debata B.K & Sahoo S, Plant Cell Rep, 15 (1996) 367.

    Article  CAS  Google Scholar 

  2. Bayliss MW, Int. Rev. Cytol. Suppl, 11A, Academic press, London (1980) pp.113–115.

    Google Scholar 

  3. Bhattacharya S, Dey T, Bandopadhyay TK & Ghosh PD, Plant Biotech Rep, 4 (2008) 245.

    Article  Google Scholar 

  4. Zietkiewicz E, Rafalski A & Labuda D, Genomics, 20 (1994) 176.

    Article  PubMed  CAS  Google Scholar 

  5. Yang WA, De-Oliveira AC, Godwin I, Schertz K & Bennetzen JL, Crop Sci, 36 (1996) 1669.

    Article  Google Scholar 

  6. Bhattacharya S, Bandopadhyay TK & Ghosh PD, Emir J Food Agric, 22 (2010) 13.

    Google Scholar 

  7. Bhatia R, Singh KP, Jhang T & Sharma TR, Sci Horti, 119 (2008) 208.

    Article  Google Scholar 

  8. Lakshmanan V, Venkataramareddy SR & Neelwarne B, Electron J Biotechnol, 10 (2007) 1.

    Article  Google Scholar 

  9. Murashige T & Skoog F, Physiol Plant, 15 (1962) 473.

    Article  CAS  Google Scholar 

  10. Gamborg OL, Plant Physiol, 45 (1970) 372.

    Article  PubMed  CAS  Google Scholar 

  11. Bhattacharya S, Bandopadhyay TK & Ghosh PD, Sci Hort, 123 (2010) 505.

    Article  CAS  Google Scholar 

  12. Khanuja SPS, Shasany AK, Darokar MP & Sushil K, Plant Mol Biol Rep, 17 (1999)1.

    Article  Google Scholar 

  13. Wolfe AD, Xiang Q-Y & Kephart SR, Proc Natl Acad Sci, USA, 95 (1998) 5112.

    Article  PubMed  CAS  Google Scholar 

  14. Nei M & Li WH, Proc Natl Acad Sci, 76 (1979) 5269.

    Article  PubMed  CAS  Google Scholar 

  15. Goto S, Thakur RC & Ishii K, Plant Cell Rep, 18 (1998) 193

    Article  CAS  Google Scholar 

  16. Rout GR, Das P, Goel S & Raina SN, Bot Bull Acad Sinica, 39 (1998) 23.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Molecular Biology and Biotechnology, University of Kalyani, Kalyani, 741235, West Bengal, India

    S. Bhattacharya & T. K. Bandopadhyay

  2. Department of Botany, University of Kalyani, Kalyani, 741235, West Bengal, India

    P. D. Ghosh

Authors
  1. S. Bhattacharya
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. K. Bandopadhyay
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. D. Ghosh
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to P. D. Ghosh.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bhattacharya, S., Bandopadhyay, T.K. & Ghosh, P.D. High Frequency Clonal Propagation of Cymbopogon martinii var motia (palmarosa) Through Rhizome Culture and True to Type Assessment using ISSR Marker. J. Plant Biochem. Biotechnol. 19, 271–273 (2010). https://doi.org/10.1007/BF03263355

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03263355

Key words

Search

Navigation