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Cysteine Protease Gene Expression and Proteolytic Activity During Floral Development and Senescence in Ethylene-insensitive Gladiolus grandiflora

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Abstract

This paper reports the changes in protein content and protease activity in the petals of ethylene-insensitive Gladiolus flowers, during development and senescence. A partial cDNA for putative cysteine protease (GgCyP) was cloned from Gladiolus using degenerate PCR primers and the expression pattern of this gene was determined semi-quantitatively by RT-PCR. There was a dramatic up-regulation in the expression of GgCyP at the incipient senescent stage of flower development indicating that this gene may encode an important enzyme for the proteolytic process in Gladiolus.

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Authors and Affiliations

  1. Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi, 110 012, India

    A. Arora & V. P. Singh

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  1. A. Arora
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  2. V. P. Singh
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Correspondence to A. Arora.

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Arora, A., Singh, V.P. Cysteine Protease Gene Expression and Proteolytic Activity During Floral Development and Senescence in Ethylene-insensitive Gladiolus grandiflora . J. Plant Biochem. Biotechnol. 13, 123–126 (2004). https://doi.org/10.1007/BF03263206

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  • DOI: https://doi.org/10.1007/BF03263206

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