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Molecular Mapping of Loci Affecting the Contents of Three Major Fatty Acids in Indian Mustard (Brassica juncea L)

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Abstract

The fatty acid constituents of mustard oil are palmitic, stearic, oleic, linoleic, linolenic and erucic acids. With the objective of mapping loci influencing the content of these fatty acids, a population of F6 generation recombinant inbred lines (RILs) derived from an inter-varietal cross of mustard was analyzed. Transgressive variation was evident for all the six fatty acids analysed irrespective of the levels of differences between the parents. The frequency distribution was normal for the linolenic acid, linoleic acid and stearic acid contents, while deviation from normality was observed for the other three fatty acids. The content of erucic acid was negatively correlated with the contents of all other fatty acids, which were positively correlated. Based on single marker analysis and interval mapping, two loci each for linoleic, linolenic and erucic acids were mapped to marker intervals on three linkage groups. Position of log of odds ratio (LOD) peaks suggested presence of common, linked and independently segregating loci for the fatty acid contents. The percentage of phenotypic variance explained by individual quantitative trait loci (QTLs) ranged from 10.5 to 19.5%, whereas the cumulative action of loci detected for different traits accounted for 16.3 to 27.6% of the variance. The additive effect for an individual locus ranged from 1.09 to 4.33. Presence of the favourable alleles at both the contributing loci in most of the RILs with a high linolenic acid content and of the unfavourable alleles in the lines with a low linolenic acid content indicated the possibility of pyramiding useful genes from phenotypically similar parental lines.

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Abbreviations

ANOVA:

analysis of variance

LOD:

log of odds ratio

PCR:

polymerase chain reaction

QTL:

quantitative trait loci

RAPD:

random amplified polymorphic DNA

RILs:

recombinant inbred lines

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Authors and Affiliations

  1. National Dairy Research Institute, Karnal, Haryana, India

    R. A. K. Aggarwal

  2. Biotechnology Centre, Rajasthan Agricultural University, Bikaner, Rajasthan, India

    R. Sharma

  3. Division of Genetics, Indian Agricultural Research Institute, New Delhi, India

    R. Kumar

  4. National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi, 110 012, India

    T. Mohapatra & R. P. Sharma

Authors
  1. R. A. K. Aggarwal
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  2. R. Sharma
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  3. R. Kumar
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  4. T. Mohapatra
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  5. R. P. Sharma
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Correspondence to T. Mohapatra.

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Aggarwal, R.A.K., Sharma, R., Kumar, R. et al. Molecular Mapping of Loci Affecting the Contents of Three Major Fatty Acids in Indian Mustard (Brassica juncea L). J. Plant Biochem. Biotechnol. 12, 131–137 (2003). https://doi.org/10.1007/BF03263173

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  • DOI: https://doi.org/10.1007/BF03263173

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