Abstract
Clonally propagated shoots of teak (Tectona grandis Linn) were cultured in vitro under photomixotrophlc (sucrose 10-40 g l-1) and photoautotrophic (sucrose-free medium) conditions in MS medium containing kinetin and benzyl amino purine (0.1 mg l-1 each). Sucrose concentrations were gradually depleted in mixotrophic cultures. Growth and fresh weight of shoot chlorophyll a, b and total chlorophyll content of leaves were estimated. In sucrose-free medium, growth and chlorophyll synthesis decreased after limited period of 2-3 subcultures, whereas they got stimulated under photomixotrophic condition with 10-30 g l-1 sucrose; optimum being the medium with 30 g l-1 sucrose. Higher concentration of sucrose (40 g l-1) inhibited shoot growth. Shoots can tolerate gradual depletion of sucrose upto a limit of 5 g l-1 under mixotrophic condition.
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Sunitibala, Y., Gupta, S. & Mukherjee, B.B. Effect of Sucrose on Growth and Chlorophyll Synthesis of Teak Shoots in Mixotrophic Culture. J. Plant Biochem. Biotechnol. 7, 57–59 (1998). https://doi.org/10.1007/BF03263036
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DOI: https://doi.org/10.1007/BF03263036