Abstract
We report here a simple procedure for the purification of the organelle DNA. Mitochondrial DNA from Sorghum and the chloroplast DNA from Populus and spinach were purified using this protocol. The method utilizes a quick centrifugation of the isolated organelle DNA through a two step CsCl density gradient for removal of small molecular weight nucleic acids which pose a major problem for getting clean restriction patterns. This method of purification can be adopted with any isolation procedure for organelle DNA.
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Nath, P., Sane, A.P., Bijola, V. et al. A Simple Method for the Purification of Organelle DNA of Plants. J. Plant Biochem. Biotechnol. 2, 117–120 (1993). https://doi.org/10.1007/BF03262937
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DOI: https://doi.org/10.1007/BF03262937