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In Vitro Comparison of the Biological Potency of Glycosylated versus Nonglycosylated rG-CSF

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Summary

The biological potency of 2 recombinant human granulocyte colony-stimulating factors (rG-CSF) formulated for clinical use (lenograstim and filgrastim) together with a nonmarketed Escherichia coli-derived rG-CSF were compared by duplicate neutrophil colony assays in 3 normal human marrows. Serial doubling dilutions of each rG-CSF (128 to 0.008 ng/ml) were used. Neutrophil colonies were blindly scored at day 14. Lenograstim stimulated neutrophil colony formation at doses 16 times lower than that of the 2 nonglycosylated rG-CSFs and was twice as potent as filgrastim at maximal colony stimulation. Qualitative differences in colony size were also noted. Both nonglycosylated rG-CSFs (filgrastim and research-use rG-CSF) gave inferior qualitative and quantitative results, suggesting that glycosylation gives lenograstim a potency advantage.

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  1. Department of Research and Department of Internal Medicine, University Hospital, Basel, Switzerland

    C. Nissen, V. Dalle Carbonare & Y. Moser

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  1. C. Nissen
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  2. V. Dalle Carbonare
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  3. Y. Moser
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Nissen, C., Dalle Carbonare, V. & Moser, Y. In Vitro Comparison of the Biological Potency of Glycosylated versus Nonglycosylated rG-CSF. Drug Invest 7, 346–352 (1994). https://doi.org/10.1007/BF03258477

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