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A comparative proteomics survey of proteins responsive to phosphorous starvation in roots of hydroponically-grown rice seedlings

  • Biochemistry
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Abstract

Rice takes up phosphorous (P) as major nutrient source for its growth and development when grown under anaerobic water-logged soil conditions. To better understand the underlying mechanisms and to develop potential protein biomarkers of P-starvation, hydroponically-grown rice seedlings in the complete media and phosphorus absence (P-starvation) of phosphorous nutrient solutions were investigated for physiological and proteome changes. The P-starvation manifested significant reduction in root growth in three-week-old seedlings compared to respective complete media. Furthermore, P-starvation also showed increased activity of acid phosphatase in roots of one- and three-week-old seedlings, suggesting that experimental design is suitable for proteomics survey of P-starvation responsive proteins. Two-dimensional gel electrophoresis coupled with matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry analysis of total root protein from three-week-old seedlings identified 10 P-starvation responsive protein spots out of 140 high-quality protein spots. Identified 10 proteins were involved in metabolism and defense/stress response. Out of 10, 2 and 8 protein spots were found to be upand down-regulated, respectively. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis of corresponding genes of four randomly selected proteins, including putative glyceraldehydes-3-phophate dehydrogenase (G3PDH, spot R1), S-adenosyl-l-methionine synthetase (SAMS, spot R4), ATP synthase subunit alpha (spot R6), and root-specific pathogenesisrelated protein 10 (PR-10, spot R8), showed that just as protein abundance, these proteins are also regulated at the transcript level. Results suggest identified P-starvation responsive proteins are involved in maintaining nutrient homeostasis and/or associated with changes in root physiology under the absence of P.

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Authors and Affiliations

  1. Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju, 660-701, Republic of Korea

    Sang Gon Kim, Sang Yeol Lee & Kyu Young Kang

  2. Division of Applied Life Science (BK21 program), Gyeongsang National University, Jinju, 660-701, Republic of Korea

    Yiming Wang, Chang Hoon Lee, Bong Gyu Mun, Pil Joo Kim, Sang Yeol Lee & Kyu Young Kang

  3. Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, 660-701, Republic of Korea

    Sang Gon Kim, Sang Yeol Lee & Kyu Young Kang

  4. School of Medicine, Showa University, 1-5-8 Hatanodai, Shinagawa, Tokyo, 142-8555, Japan

    Randeep Rakwal

  5. Research Laboratory for Biotechnology and Biochemistry (RLABB), GPO Box 13265, Kathmandu, Nepal

    Randeep Rakwal & Ganesh Kumar Agrawal

  6. Department of Plant Bioscience, Pusan National University, Miryang, 627-706, Republic of Korea

    Yong Chul Kim & Sun Tae Kim

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  1. Sang Gon Kim
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Kim, S.G., Wang, Y., Lee, C.H. et al. A comparative proteomics survey of proteins responsive to phosphorous starvation in roots of hydroponically-grown rice seedlings. J Korean Soc Appl Biol Chem 54, 667–677 (2011). https://doi.org/10.1007/BF03253144

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