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DNA-Binding and cytotoxicity of the cobalt(III) ethylenediamine pyrazole complex [Co(en)2(pyz)2]3+

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Abstract

The complex of cobalt(III) ethylenediamine was synthesized, isolated and characterized by UV-Vis, IR, and 1H NMR spectral methods. The binding of the complex with calf thymus DNA was investigated by absorption and emission spectroscopy, viscosity measurements, DNA melting and DNA photocleavage. The spectroscopic studies together with the viscosity measurements and DNA melting studies indicated that the complex binds to calf thymus DNA in a nonintercalative mode. This complex is found to promote photocleavage of the DNA plasmid pBR322 and shows a cytotoxic effect against CHO cells.

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References

  1. L.-N. Ji, X.-H. Zou, J.-G. Liu, Coord. Chem. Rev. 216- 217 (2001) 513.

    CAS  Google Scholar 

  2. N.A.P. Kane-Maguire, J.F. Wheeler, Coord. Chem. Rev. 211 (2001) 145.

    CAS  Google Scholar 

  3. K.E. Erkkila, D.T. Odom, J.K. Barton, Chem. Rev. 99 (1999) 2777.

    CAS  Google Scholar 

  4. Y. Xiong, L.-N. Ji, Coord. Chem. Rev. 185/186 (1999) 711.

    Google Scholar 

  5. S.O. Kelly, J.K. Barton, in: A. Sigel, H. Sigel (Eds.), Metal Ions in Biological Systems, Vol. 39, Marcel Dekker, New York, 1998, pp. 211–249.

    Google Scholar 

  6. B. Norden, P. Lincoln, B. Akerman, E. Tuite, in: A. Sigel, H. Sigel (Eds.), Metal Ions in Biological Systems, Vol. 33, Marcel Dekker, New York, 1996, pp. 177–252.

    Google Scholar 

  7. A.K. Mesmaeker, J.P. Lecomte, J.M. Kelly, in: J. Mattay (Ed.), Topics in Current Chemistry, Vol. 177, 1996, pp. 25–76.

    Google Scholar 

  8. H.H. Thorp, Adv. Inorg. Chem. 43 (1995) 127.

    CAS  Google Scholar 

  9. D.S. Sigman, A. Mazumder, D.M. Perrin, Chem. Rev. 93 (1993) 2295.

    CAS  Google Scholar 

  10. P. Nagababu, J.N. Lavanyalatha, S. Satyanarayana, Chem. Biodivers. 3 (2006) 1219.

    CAS  Google Scholar 

  11. J.K. Barton, Science 233 (1986) 727.

    CAS  Google Scholar 

  12. C.S. Chow, J.K. Barton, J. Am. Chem. Soc. 112 (1990) 2839.

    CAS  Google Scholar 

  13. X. Qu, J.B. Chaires, J. Am. Chem. Soc. 123 (2001) 1.

    CAS  Google Scholar 

  14. P. Nagababu, S. Satyanarayana, Polyhedron 26 (2007) 1686–1692.

    CAS  Google Scholar 

  15. Y. Xiong, L.N. Ji, Coord. Chem. Rev. 185 (1996) 711.

    Google Scholar 

  16. J.K. Barton, J. Biomol. Struct. Dyn. 1 (1983) 621.

    CAS  Google Scholar 

  17. J.K. Barton, A.L. Raphael, J. Am. Chem. Soc. 106 (1984) 2466.

    CAS  Google Scholar 

  18. A. Barve, S. Ghosh, A. Kumbhar, A. Kumbhar, V. Puranik, Tans. Metal Chem. 30 (2005) 312.

    CAS  Google Scholar 

  19. J. Marmur, J. Mol. Biol. 3 (1961) 208.

    CAS  Google Scholar 

  20. M.F. Reichmann, S.A. Rice, C.A. Thomas, P. Doty, J. Am. Chem. Soc. 76 (1954) 3047.

    CAS  Google Scholar 

  21. W.C. Fernelius, Inorg. Synth. 2 (1946) 222.

    Google Scholar 

  22. A.C. Barve, S. Ghosh, A.A.A. Avinash Kumbhar, A.S. Kumbhar, Trans. Metal Chem. 30 (2005) 312.

    CAS  Google Scholar 

  23. P. Nagababu, J. Naveena Lavanya Latha, P. Pallavi, S. Harish, S. Satyanarayana, Can. J. Microbiol. 52 (2006) 1247.

    CAS  Google Scholar 

  24. P. Nagababu, D. Aravind Kumar, K. Laxma Reddy, K. Ashwini Kumar, Md.B. Mustafa, M. Shlipa, S. Satyanarayana, Metal Based Drugs 2008, Articale ID 75084, p. 8.

    Google Scholar 

  25. J.B. Chaires, N. Dattaguota, D.M. Crothers, Biochem. 21 (1982) 3940.

    Google Scholar 

  26. S. Satyanarayana, J.C. Dabrowik, J.B. Chaires, Biochem. 32 (1993) 2573.

    CAS  Google Scholar 

  27. P.A. Sharp, B. Sugden, J. Sambrook, Biochem. 12 (1973) 3055.

    CAS  Google Scholar 

  28. S.T. Rapid, J. Immunol. Methods 65 (1983) 55.

    Google Scholar 

  29. K. Nazeeruddin, S.M. Zakeeruddin, R. Humphry- Baker, S.I. Gorelsky, A.B.P. Lever, M. Gratzel, Coord. Chem. Rev. 208 (2000) 213.

    CAS  Google Scholar 

  30. M. Wolfe, G.H. Shimer, T. Meehan, Biochemistry 26 (1987) 6392.

    CAS  Google Scholar 

  31. S. Satyanarayana, J.C. Dabrowiak, J.B. Chaires, Biochem. 31 (1992) 9319.

    CAS  Google Scholar 

  32. J.M. Kelly, A.B. Tossi, D.J. McConnell, C. OhUigin, Nucleic Acids Res. 13 (1985) 6017.

    CAS  Google Scholar 

  33. E. Tselepi-Kalouli, N. Katsaros, J. Inorg. Biochem. 37 (1989) 271.

    CAS  Google Scholar 

  34. R.B. Lopez, B.L. Loen, T. Boussie, T.J. Meyer, Tetrahedron Lett. 37 (1996) 5437.

    Google Scholar 

  35. D.S. Sigman, D.R. Graham, L. E. Marshall, K.A. Reich, J. Am. Chem. Soc. 102 (1980) 5490.

    Google Scholar 

  36. J.K. Barton, A.L. Raphael, J. Am. Chem. Soc. 106 (1984) 2466.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry, Osmania University, Hyderabad, Andhra Pradesh, India

    P. Nagababu, J. Naveena lavanya latha & S. Satyanarayana

  2. Department of Biochemistry, Osmania University, Hyderabad, Andhra Pradesh, India

    M. Rajesh

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  1. P. Nagababu
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  2. J. Naveena lavanya latha
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  3. M. Rajesh
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  4. S. Satyanarayana
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Correspondence to S. Satyanarayana.

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Nagababu, P., Naveena lavanya latha, J., Rajesh, M. et al. DNA-Binding and cytotoxicity of the cobalt(III) ethylenediamine pyrazole complex [Co(en)2(pyz)2]3+ . JICS 6, 145–152 (2009). https://doi.org/10.1007/BF03246513

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  • DOI: https://doi.org/10.1007/BF03246513

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