Skip to main content
SpringerLink
Log in

Behavior of hepatocytes inoculated in gelatin-immobilized polyurethane foam

  • Published:
Macromolecular Research Aims and scope Submit manuscript

Abstract

We have fabricated gelatin-immobilized polyurethane foams (PUFG) by dipping polyurethane foam (PUF) in an aqueous solution containing gelatin and by subsequent reaction with glutaraldehyde after freeze-drying. Gelatin aqueous solutions of different concentrations were used as the dipping solutions to control the amount of immobilized gelatin. The average pore size of PUF decreased with an increase in gelatin concentration. It was found from the hepatocyte adhesion experiment that the amount of hepatocytes seeded on PUFG1, prepared by using a 1% aqueous gelatin solution, was higher than that on other PUFGs. The hepatocytes inoculated in PUFG1 were slightly aggregated as the incubation time increased. The cells inoculated in PUFG1 showed higher ammonia removal ability than those monolayer-cultured on a gelatin-immobilized polystyrene dish (PSG) after 1 and 4 days of incubation time. The inoculated cells exhibited higher albumin secretion relative to monolayer-cultured hepatocytes on PSG. Albumin secretion by hepatocytes seeded on PUFG1 was increased by the presence of serum and was further increased by both the presence of serum and cytokines. The results obtained from a 3-(3,4-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay indicated that PUFG can provide a better microenvironment for hepatocyte culture along with nutrition and metabolite transfer through the high porosity of PUF.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. K. Nakazawa, H. Ijima, J. Fukuda, R. Sakiyama, Y. Yamashita, M. Shimada, K. Shirabe, E. Tsujita, K. Sugimachi, and K. Funatsu,Int. J. Artif. Organs,25, 51 (2002).

    CAS  Google Scholar 

  2. J. Fukuda, R. Sakiyama, K. Nakazawa, H. Ijima, Y. Yamashita, M. Shimada, K. Shirabe, E. Tsujita, K. Sugimachi, and K. Funatsu,Int. J. Artif. Organs,24, 799 (2001).

    CAS  Google Scholar 

  3. K. Funatsu, H. Ijima, K. Nakazawa, Y. Yamashita, M. Shimada, and K. Sugimachi,Artif. Organs,25, 194 (2001).

    Article  CAS  Google Scholar 

  4. Y. Yamashita, M. Shimada, E. Tsujita, T. Rikimaru, H. Ijima, K. Nakazawa, R. Sakiyama, J. Fukuda, and K. Funatsu,Int. J. Artif. Organs,24, 34 (2001).

    CAS  Google Scholar 

  5. Y. Yamashita, M. Shimada, E. Tsujita, S. Tanaka, H. Ijima, K. Nakazawa, R. Sakiyama, J. Fukuda, T. Ueda, and K. Funatsu,Cell Transp.,10, 717 (2001).

    CAS  Google Scholar 

  6. H. Kurosawa, K. Yasumoto, T. Kimura, and Y. Amano,Biotech. Bioeng.,70, 160 (2000).

    Article  CAS  Google Scholar 

  7. H. Ijima, K. Nakazawa, S. Koyama, M. Kaneko, T. Matsushita, T. Gion, K. Shirabe, M. Shimada, K. Takenaka, K. Sugimachi, and K. Funatsu,Int. J. Artif. Organs,23, 446 (2000).

    CAS  Google Scholar 

  8. H. Ijima, K. Nakazawa, S. Koyama, M. Kaneko, T. Matsushita, T. Gion, K. Shirabe, M. Shimada, K. Takenaka, K. Sugimachi, and K. Funatsu,Int. J. Artif. Organs,23, 389 (2000).

    CAS  Google Scholar 

  9. Y. Fujii, K. Nakazawa, and K. Funatsu,J. Biomater. Sci., Polymer Edn.,11, 731 (2000).

    Article  CAS  Google Scholar 

  10. H. Ijima, K. Nakazawa, H. Mizumoto, T. Matsushita, and K. Funatsu,J. Biomater. Sci., Polymer Edn.,9, 765 (1998).

    Article  CAS  Google Scholar 

  11. T. Matsushita, H. Ijima, N. Koide, and K. Funatsu,Appl. Microbio. Biotech.,36, 324 (1991).

    CAS  Google Scholar 

  12. Y. Ikada and T. Okano,Tissue Engineering for Therapeutic Use 3, Elsevier Scientific Publishing Co., Amsterdam, 1999.

    Google Scholar 

  13. M. Kaneko, J. Fukuda, H. Ijima, K. Nakazawa, T. Gion, M. Shimada, K. Shirabe, K. Takenaka, K. Sugimachi, and K. Funatsu.Mater. Sci. Eng.,C6, 245 (1998).

    Article  Google Scholar 

  14. H. Ijima, T. Matsushita, K. Nakazawa, Y. Fujii, and K. Funatsu,Tissue. Eng.,4, 213 (1998).

    Article  CAS  Google Scholar 

  15. T. Gion, M. Shimada, M. Shimada, K. Shirabe, K. Nakazawa, H. Ijima, T. Matsushita, K. Funatsu, and K. Sugimachi,J. Surg. Res.,82, 131 (1999).

    Article  CAS  Google Scholar 

  16. Y. Sato, T. Ochiya, Y. Yasuda, and K. Matsubara,Hepatology,19, 1023 (1994).

    Article  CAS  Google Scholar 

  17. J. A. Rowley, G. Madlabayan, and D. J. Mooney,Biomaterials,20, 45 (1999).

    Article  CAS  Google Scholar 

  18. S. T. Lopina, G. Wu, E. W. Merrill, and L. G. Cima,Biomaterials,17, 559 (1996).

    Article  CAS  Google Scholar 

  19. T. Matsuda and M. J. Moghaddam,J. Polym. Sci. Polym. Chem.,31, 1589 (1993).

    Article  Google Scholar 

  20. Y. C. Lee and R. T. Lee,Neoglycoconjugates: Preparation and Application, Academic Press, San Diego, 1994.

    Google Scholar 

  21. B. J. Nathan and P. J. Evans,Cryobiology,40, 176 (2000).

    Article  Google Scholar 

  22. P. J. Evans,Cell. Biol. Intern.,19, 855 (1995).

    Article  CAS  Google Scholar 

  23. S. Zmora, R. Glicklis, and S. Cohen,Biomaterials,23, 4087 (2002).

    Article  CAS  Google Scholar 

  24. J. Yang, M. Goto, H. Ise, C. S. Cho, and T. Akaike,Biomaterials,23, 471 (2002).

    Article  CAS  Google Scholar 

  25. Y. Ikada and T. Okano,Tissue Engineering for Therapeutic Use 3, Elsevier Scientific Publishing Co., Amsterdam, 1999.

    Google Scholar 

  26. N. Ohshima, K. Yanagi, and H. Miyoshi,Artif. Organs,21, 1169 (1997).

    Article  CAS  Google Scholar 

  27. K. Yanagi, S. Mizuno, and N. Ohshima,Trans. Am. Soc. Artif. Intern. Organs,36, 727 (1990).

    Google Scholar 

  28. K. Yanagi, H. Miyoshi, H. Fukuda, and N. Ohshima,Appl. Microbiol. Biotech.,37, 316 (1992).

    Article  CAS  Google Scholar 

  29. S. J. Lee, Y. M. Lee, G. Khang, I. Y. Kim, B. Lee, and H. B. Lee,Macromol. Res.,10, 150 (2002).

    Article  CAS  Google Scholar 

  30. G. Khang, C. S. Park, J. M. Lee, S. J. Lee, Y. M. Lee, M. K. Choi, I. Lee, and H. B. Lee,Korea Polym. J.,9, 267, (2001).

    CAS  Google Scholar 

  31. P. O. Seglen,Methods Cell Biol.,13, 23 (1976).

    Google Scholar 

  32. A. T. Gutsche, H. L. Zurlo, J. J. Yager, and K. W. Leong,Biomaterials,17, 387 (1996).

    Article  CAS  Google Scholar 

  33. J. Yang, T. W. Chung, M. Nagaoka, M. Goto, C. S. Cho, and T. Akaike,Biotechnol. Lett.,23, 1385 (2001).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Polymer Science, Kyungpook National University, 702-701, Daegu, Korea

    Kyung-Su Yang, Xinglin Guo, Wan Meng, Jae-Yong Hyun & Inn-Kyu Kang

  2. School of Medicine, Kyungpook National University, 700-091, Daegu, Korea

    Yang Il Kim

Authors
  1. Kyung-Su Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xinglin Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wan Meng
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jae-Yong Hyun
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Inn-Kyu Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yang Il Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yang, KS., Guo, X., Meng, W. et al. Behavior of hepatocytes inoculated in gelatin-immobilized polyurethane foam. Macromol. Res. 11, 488–494 (2003). https://doi.org/10.1007/BF03218981

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03218981

Keywords

Search

Navigation