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Preparation and characterization of new immunoprotecting membrane coated with amphiphilic multiblock copolymer

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Abstract

New immunoprotecting membranes were prepared by spin coating the amphiphilic random multiblock copolymers of poly(ethylene glycol) (PEG) and poly(tetramethylene ether glycol) (PTMEG) or poly(dimethyl siloxane) (PDMS) on porous Durapore® membrane. The copolymer coating was intended to make a biocompatible, immunoprotecting diffusional barrier and the supporting porous substrate was for mechanical stability and processability. By filling Durapore® membrane pores with water, the penetration of coating solution into the pores was minimized during the spin coating process. A single coating process produced a completely covered thin surface layer (∼1 μm in thickness) on the porous substrate membrane. The permselectivity of the coated layer was influenced by PEG block length, polymer composition, and thickness of the coating layer. A composite membrane with the coating layer prepared with PEG 2 K/PTMEG 2 K block copolymer showed that its molecular weight cut-off at day 40 based on dextran was close to the molecular size of IgG (M w = 150 kDa). However, IgG permeation was detected from protein permeation test, while glucose oxidase (M w = 186 kDa) was not permeable through the coated membrane.

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Authors and Affiliations

  1. Center for Biomaterials and Biotechnology Department of Materials Science and Engineering, Kwangju Institute of Science and Technology, 1 Oryong-dong, Puk-gu, 500-712, Kwangju, Korea

    Han Chang Kang & You Han Bae

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  1. Han Chang Kang
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  2. You Han Bae
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Dedicated to Dr. Un Young Kim, on the occasion of his retirement.

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Kang, H.C., Bae, Y.H. Preparation and characterization of new immunoprotecting membrane coated with amphiphilic multiblock copolymer. Macromol. Res. 10, 67–74 (2002). https://doi.org/10.1007/BF03218292

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  • DOI: https://doi.org/10.1007/BF03218292

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