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Inhibitory effects of 1α, 25dihydroxyvitamin D3 and Ajuga iva extract on oxidative stress, toxicity and hypo-fertility in diabetic rat testes

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Abstract

The aim of the eurrent study is to investigate the therapeutic and preventive effects of 1α, 25dihydroxyvitaminD3 (1,25 (OH)2 D3) andAjuga iva (AI) extraet on diabetes toxicity in rats testes. Thus diabetic rats were treated with 1α, 25dihydroxyvitaminD3 orAjuga iva extract as both therapeutie and preventive treatments on diabetes toxicity in rats testes. Our results showed that diabetes indueed a decrease in testosterone and 17β-estradiol levels in testes and plasma. Besides, a fall in testicular antioxidant capacity appeared by a deerease in both antioxidant (superoxide dismutase (SOD), eatalase (CAT) and glutathione peroxidase (GPx) activities) and non-enzymatic antioxidant (copper (Cu), magnesium (Mg) and iron (Fe) levels). All theses changes enhanced testicular toxicity (inerease in testicular aspartate amino transaminase (AST), alanine amino transaminase (ALT), laetate dehydrogenase (LDH) activities and the lipid peroxidation and triglyeeride (TG) levels). In addition, a decrease in testicular total cholesterol (TCh) level was observed in diabetic rats testes. All the ehanges lead to a decrease in the total number and mobility of epididymal spermatozoa. The administration of 1α,25dihydroxyvitaminD3 andAjuga iva extract three weeks before and after diabetes induetion interfered and prevented diabetes toxicity in the reproduetive system. 1,25 (OH)2 D3 andAjuga iva extract blunted all changes observed in diabetic rats. To sum up, the data suggested that 1,25 (OH)2 D3 andAjuga iva extract have a protective effect on alloxan-induced damage in reproductive system by enhancing the testosterone and 17β-estradiol levels, consequenty protecting from oxidative stress, cellular toxicity and maintaining the number and motility of spermatozoids.

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Authors and Affiliations

  1. Faculty of Seienees, Animal Ecophysiology, Sfax, Tunisia

    K. Hamden & A. Elfeki

  2. USC 2006 INRA- EA 2608, Biochemistry-University of Caen, France

    S. Carreau

  3. ]Biochemistry laboratory, CHU H. Bourguiba, Sfax, Tunisia

    K. Jamoussi & F. Ayadi

  4. Radio-immunology laboratory, CHU H. Bourguiba, Sfax, Tunisia

    F. Garmazi & N. Mezgenni

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  1. K. Hamden
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  2. S. Carreau
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  3. K. Jamoussi
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  4. F. Ayadi
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  7. A. Elfeki
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Correspondence to K. Hamden.

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Hamden, K., Carreau, S., Jamoussi, K. et al. Inhibitory effects of 1α, 25dihydroxyvitamin D3 and Ajuga iva extract on oxidative stress, toxicity and hypo-fertility in diabetic rat testes. J Physiol Biochem 64, 231–239 (2008). https://doi.org/10.1007/BF03216108

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  • DOI: https://doi.org/10.1007/BF03216108

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