Abstracts
Adipose triglyceride lipase (ATGL) catalyses the initial step in triglyceride hydrolysis, so theATGL gene is a candidate for growth and fat traits in chickens. Nine reported single-nucleotide polymorphisms (SNPs) located in 3 exons of the chickenATGL gene were chosen for genotyping an F2 population. Only 5 SNPs were confirmed for polymorphisms and used for association analyses. The results show that c.531G>A (p.E177Syn) was not associated with any growth and fat traits (P > 0.05), but c.782G>A (p.S261N) was associated with body weight (BW) on days 14, 21, 35, 63, 70, 77, cingulated fat width and abdominal fat pad weight (P< 0.05), and significantly associated with BW on days 42, 49, and 56 (P < 0.01). Significant associations of c.903C>T (p.F301Syn) with BW on days 49 and 77 days and crude protein content of breast muscle (P < 0.05), and c. 1164G>A (p.K388Syn) with BW on day 7 (P< 0.05) were also detected. Additionally, c. 1069T>C (p.L357Syn) was associated with breast muscle colour (P < 0.05), and significantly associated with crude fat (ether extract) content of breast muscle (P< 0.01). Thus the missense SNP of c.782G>A (p.S261N) was significantly associated with the largest number of chicken growth and fat traits in this study.
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References
Ahn K, Gordon D, Finch SJ, 2009. Increase of rejection rate in case-control studies with the differential genotyping error rates. Stat Appl Genet Mol Biol 8: Article 25.
Chen JF, Dai LH, Xu NY, Xiong YZ, Jiang SW, 2006. Assignment of the patatin-like phospholipase domain containing 2 gene (PNPLA2) to porcine chromosome 2p17 with radiation hybrids. Cytogenet Genome Res 112: 342G.
Deiuliis JA, Shin J, Bae D, Azain MJ, Barb R, Lee K, 2008. Developmental, hormonal, and nutritional regulation of porcine adipose triglyceride lipase (ATGL). Lipids 43: 215–225.
Fischer J, Lefèvre C, Morava E, Mussini JM, Laforêt P, Negre-Salvayre A, et al. 2007. The gene encoding adipose triglyceride lipase (PNPLA2) is mutated in neutral lipid storage disease with myopathy. Nat Genet 39: 28–30.
Jenkins CM, Mancuso DJ, Yan W, Sims HF, Gibson B, Gross RW, 2004. Identification, cloning, expression, and purification of three novel human calcium-independent phospholipase A2 family members possessing triacylglycerol lipase and acylglycerol transacylase activities. J Biol Chem 279: 48968–48975.
Kienesberger PC, Oberer M, Lass A, Zechner R, 2009. Mammalian patatin domain containing proteins: a family with diverse lipolytic activities involved in multiple biological functions. J Lipid Res 50: 63–68.
Lass A, Zimmermann R, Haemmerle G, Riederer M, Schoiswohl G, Schweiger M, et al. 2006. Adipose triglyceride lipase-mediated lipolysis of cellular fat stores is activated by CGI-58 and defective in Chanarin-Dorfman syndrome. Cell Metab 3: 309–319.
Lee K, Shin J, Latshaw JD, Suh Y, Serr J, 2009. Cloning of adipose triglyceride lipase complementary deoxyribonucleic acid in poultry and expression of adipose triglyceride lipase during development of adipose in chickens. Poult Sci 88: 620–630.
Lei M, Nie Q, Peng X, Zhang D, Zhang X, 2005. Single nucleotide polymorphisms of the chicken insulin-like factor binding protein 2 gene associated with chicken growth and carcass traits. Poult Sci 84: 1191–1198.
Lettre G, Lange C, Hirschhorn JN, 2007. Genetic model testing and statistical power in population-based association studies of quantitative traits. Genet Epidemiol 31: 358–362.
Nemes S, Jonasson JM, Genell A, Steineck G, 2009. Bias in odds ratios by logistic regression modelling and sample size. BMC Med Res Methodol 9: 56.
Nie Q, Fang M, Xie L, Shi J, Zhang X, 2009. cDNA cloning, characterization, and variation analyses of chicken adipose triglyceride lipase (ATGL) gene. Mol Cell Biochem 320: 67–74.
Okazaki H, Osuga J, Tamura Y, Yahagi N, Tomita S, Shionoiri F, et al. 2002. Lipolysis in the absence of hormone-sensitive lipase: evidence for a common mechanism regulating distinct lipases. Diabetes 51: 3368–3375.
Saarela J, Jung G, Hermann M, Nimpf J, Schneider WJ, 2008. The patatin-like lipase family inGallus gallus. BMC Genomics 9: 281.
Schoenborn V, Heid IM, Vollmert C, Lingenhel A, Adams TD, Hopkins PN, et al. 2006.The ATGL gene is associated with free fatty acids, triglycerides, and type 2 diabetes. Diabetes 55: 1270–1275.
Shan T, Wang Y, Wu T, Guo J, Liu J, Feng J, Xu Z, 2008. Porcine adipose triglyceride lipase complementary deoxyribonucleic acid clone, expression pattern, and regulation by resveratrol. J Anim Sci 86: 1781–1788.
Subramanian S, 2008. Nearly neutrality and the evolution of codon usage bias in eukaryotic genomes. Genetics 178: 2429–2432.
Villena JA, Roy S, Sarkadi-Nagy E, Kim KH, Sul HS, 2004. Desnutrin, an adipocyte gene encoding a novel patatin domain-containing protein, is induced by fasting and glucocorticoids: ectopic expression of desnutrin increases triglyceride hydrolysis. J Biol Chem 279: 47066–47075.
Wang SP, Laurin N, Himms-Hagen J, Rudnicki MA, Levy E, Robert MF, et al. 2001. The adipose tissue phenotype of hormone-sensitive lipase deficiency in mice. Obes Res 9: 119–128.
Zimmermann R, Strauss JG, Haemmerle G, Schoiswohl G, Birner-Gruenberger R, Riederer M, et al. 2004. Fat mobilization in adipose tissue is promoted by adipose triglyceride lipase. Science 306: 1383–1386.
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Nie, Q.H., Fang, M.X., Xie, L. et al. Associations of ATGL gene polymorphisms with chicken growth and fat traits. J Appl Genet 51, 185–191 (2010). https://doi.org/10.1007/BF03195726
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DOI: https://doi.org/10.1007/BF03195726