Abstract
Thirteen candidate genes for human obesity were selected for cytogenetic mapping by FISH in the pig genome. Among them, 6 genes were assigned to chromosomes for the first time (NR3C1, GNB3, ADRB1, ADRB2, ADRB3 andUCP1). Location of the other 7 genes (INSIG2, LIPIN1, PLIN, NAMPT, ADIPOQ, UCP2 andUCP3), earlier mapped by somatic cell hybridization or with the use of a radiation hybrid panel, was verified (INSIG2) or more precisely described. The genes were assigned to the following chromosomes:INSIG2 to SSC15q12,LIPIN1 to SSC3q26,NR3C1 to SSC2q29,PLIN to SSC7q15,GNB3 to SSC5q21,NAMPT to SSC9q23,ADIPOQ to SSC13q41,ADRB1 to SSC14q28,ADRB2 to SSC2q29,ADRB3 to SSC15q13-14,UCP1 to SSC8q21-22, and bothUCP2 andUCP3 to SSC9p24. Most of the genes were located within known QTL for pig fatness traits.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bruskiewich R, Everson T, Ma L, Chan L, Schertzer M, Giacobino JP, et al. 1996. Analysis of CA repeat polymorphisms places three human gene loci on the 8p linkage map. Cytogenet Cell Genet 73: 331–333
Cassard AM, Bouillaud F, Mattei MG, Hentz E, Raimbault S, Thomas M, Ricquier D, 1990. Human uncoupling protein gene: structure, comparison with rat gene and assignment to the long arm of chromosome 4. J Cell Biochem 43: 255–264.
Cepica S, Bratenschlager H, Ovilo C, Fernandez A, Zrustova J, Masopust M, et al. 2008. SNP within the porcineNAMPT/PBEF1/visfatin gene is associated with fat and carcass traits. Abstracts of the 31st Conference of the International Society for Animal Genetics, 20–24 July 2008, Amsterdam, The Netherlands, poster 2039.
Clarke IJ, 2008. Models of ‘obesity’ in large animals and birds. Front Horm Res 36: 107–117.
Croojmans RPMA, Ramos AM, Amaral AJ, Kerstens H, Bendixen C, Hedegaard J, et al. 2008. High throughput SNP discovery and validation in the pig: towards the development of a swine 60K SNP chip. Abstracts of the 31st Conference of the International Society for Animal Genetics, 20–24 July 2008, Amsterdam, The Netherlands, poster 2234.
Dai MH, Xia T, Zhang GD, Chen XD, Gan L, Feng SQ, et al. 2006. Cloning, expression and chromosome localization of porcine adiponectin and adiponectin receptors genes. Domest Anim Endocrinol 30: 117–125.
Dahlman I, Arner P, 2007. Obesity and polymorphisms in genes regulating human adipose tissue. Int J Obes (Lond)31: 1629–1641.
Goureau A, Yerle M, Schmitz A, Riquet J, Milan D, Pinton P, et al. 1996. Human and porcine correspondence of chromosome segments using bidirectional chromosome painting. Genomics 36: 252–262.
Gustavsson I (co-ordinator), 1988. Standard karyotype of the domestic pig. Committee for the Standardized Karyotype of the Domestic Pig. Hereditas 109: 151–157.
He XP, Xu XW, Zhao SH, Fan B, Yu M, Zhu MJ, et al. 2008. Investigation ofLpin1 as a candidate gene for fat deposition in pigs. Mol Biol Rep DOI: 10.1007/s1 1033-008-9294-4.
Ichihara S, Yamada Y, 2008. Genetic factors for human obesity. Cell Mol Life Sci 65: 1086–1098.
Karnuah AB, Uenishi H, Kiuchi S, Kojima M, Onishi A, Yasue H, Mitsuhashi T, 2001. Assignment of 64 genes expressed in 28-day-old pig embryo to radiation hybrid map. Mamm Genome 12: 518–523.
Li H, Li Y, Zhao X, Li N, Wu C, 2005. Structure and nucleotide polymorphisms in pig uncoupling protein 2 and 3 genes. Anim Biotechnol 16: 209–220.
Li Y, Li H, Zhao X, Li N, Wu C, 2007. UCP2 and 3 deletion screening and distribution in 15 pig breeds. Biochem Genet 45: 103–111.
Lunney JK, 2007. Advances in swine biomedical model genomics. Int J Biol Sci 3: 179–184.
Martinez-Hernandez A, Enriquez L, Moreno-Moreno MJ, Marti A, 2007. Genetics of obesity. Public Health Nutr 10: 1138–1144.
Mutch DM, Clement K, 2006. Unraveling the genetics of human obesity. PLoS Genet 2: e188.
Pertek A, Schwarzenbacher H, Flisikowski K, Fries R, 2008. Insulin induced gene 2 (INSIG2) is associated with fatty acid composition in the pig. Abstracts of the 31st Conference of the International Society for Animal Genetics, 20–24 July 2008, Amsterdam, The Netherlands, poster 2235.
Qiu H, Xia T, Chen XD, Feng SQ, Gan L, Lei T, et al. 2005. Sequencing and chromosome mapping of pig INSIG 2 and a related pseudogene. Anim Genet 36: 188–189.
Rothschild MF, Hu ZL, Jiang Z, 2007. Advances in QTL mapping in pigs. Int J Biol Sci 3: 192–197.
Solanes G, Vidal-Puig A, Grujic D, Flier JS, Lowell BB, 1997. The human uncoupling protein-3 gene. J Biol Chem 272: 25433–25436.
Szczerbal I, Lin L, Stachowiak M, Chmurzynska A, Mackowski M, Winter A, et al. 2007a. Cytogenetic mapping of DGAT1, PPARA, ADIPOR1 and CREB genes in the pig. J Appl Genet 48: 73–76.
Szczerbal I, Chmurzynska A, Switonski M, 2007b. Cytogenetic mapping of eight genes encoding fatty acid binding proteins (FABPs) in the pig genome. Cytogenet Genome Res 118: 63–66.
Szczerbal I, Chmurzynska A, 2008. Chromosomal localization of nine porcine genes encoding transcription factors involved in adipogenesis. Cytogenet Genome Res 121: 50–54.
Tao X, Jihong Y, Li G, Bin F, Yi Z, Xiaodong C, et al. 2008. Cloning, chromosome mapping and expression pattern of porcine PLIN and M6PRBP1 genes. Genet Sel Evol 40: 215–226.
Yang-Feng TL, Xue FY, Zhong WW, Cotecchia S, Frielle T, Caron MG, et al. 1990. Chromosomal organization of adrenergic receptor genes. Proc Natl Acad Sci U S A. 87: 1516–1520.
Werner P, Neuenschwander S, Stranzinger G, 1999. Characterization of the porcine uncoupling proteins 2 and 3 (UCP2 & UCP3) and their localization to chromosome 9p by somatic cell hybrids. Anim Genet 30: 221–224.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Nowacka-Woszuk, J., Szczerbal, I., Fijak-Nowak, H. et al. Chromosomal localization of 13 candidate genes for human obesity in the pig genome. J Appl Genet 49, 373–377 (2008). https://doi.org/10.1007/BF03195636
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF03195636