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Verification of reference genes for relative quantification of gene expression by real-time reverse transcription PCR in the pig

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Abstract

The aim of this study was to develop a set of reliable reference genes for quantification of mRNA expression in the pig. The mRNA expression stability in pig tissues was studied for 4 genes:EEF1A1, GAPDH, HPRT1 andTOP2B. The level of expression was characterized byCt values for each gene and each tissue. By using the geNorm algorithm, the stability of the reference genes was determined in the diaphragm, heart, kidney, liver, lungs, longissimus muscle, and spleen. On the basis of this information, suitable reference genes can be selected for mRNA expression studies in relevant pig tissues.

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Authors and Affiliations

  1. Department of Animal Morphology, Physiology and Genetics, Mendel University of Agriculture and Forestry, Brno, Czech Republic

    Kateřina Svobodová, Karel Bílek & Aleš Knoll

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  1. Kateřina Svobodová
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  2. Karel Bílek
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  3. Aleš Knoll
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Correspondence to Kateřina Svobodová.

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Svobodová, K., Bílek, K. & Knoll, A. Verification of reference genes for relative quantification of gene expression by real-time reverse transcription PCR in the pig. J Appl Genet 49, 263–265 (2008). https://doi.org/10.1007/BF03195623

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  • DOI: https://doi.org/10.1007/BF03195623

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