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Production of zearalenone, nivalenol, moniliformin, and wortmannin from toxigenic cultures ofFusarium obtained from pasture soil samples collected in New Zealand

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Abstract

One culture ofF avenaceum, 4 cultures ofF oxysporum, and 11 cultures of Fsambucinum were isolated from soil samples of pasture in New Zealand in 1987. All cultures, when grown on rice media and fed to rats caused a weight loss in rats as well as toxic signs including hemorrhaging and congestion, uterine enlargement, and hematuria. 6 out of 16 cultures caused death in rat feeding tests.F oxysporum #1 killed rats (feeding test) within 5-12hrs. 10 cultures produced zearalenone (19 to 8,849 ppm), 8 cultures produced nivalenol (32 to 117 ppm), 1 culture,F sambucinum #8, produced wortmannin (40 ppm), and 5 cultures produced moniliformin (19 to 9,000ppm). We report for the first time the co-occurrence of zearalenone, nivalenol, and moniliformin produced byF sambucinum #3 in culture.F avenaceum #1 andF oxysporum cultures (nos 1, 2, and 3) produced moniliformin alone.F oxysporum #4 produced zearalenone alone as well.F sambucinum #5 caused erythema in the small intestine of rats and 100% mortality and did not produce any known toxin(s). Nivalenol when administered to the stomach of rats orally at levels 10, 20, and 40mg/kg body weight caused inflammation in the intestines, coma, and death. The mycotoxins T-2 toxin, HT-2 toxin, T-2 tetraol, diacetoxyscirpenol (DAS), monoacetoxyscirpenol (MAS), deoxynivalenol (DON), 3-acetyl-and 15-acetyldeoxynivalenol, depoxynivalenol, fusarenon-X, alpha-and beta-zearalenone, and fusarochromanone (TDP-1) were not detected in the extracts of these cultures.

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Authors and Affiliations

  1. Southern Weed Science Lab, USDA-ARS, 38776, Stoneville, MS, USA

    H K Abbas

  2. Departments of Plant Pathology and School of Medicine, University of Minnesota, 55108, St Paul, MN, USA

    C J Mirocha

  3. Laboratory Medicine and Pathology, School of Medicine, University of Minnesota, 55108, St Paul, MN, USA

    R Gunther

Authors
  1. H K Abbas
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  2. C J Mirocha
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  3. R Gunther
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Abbas, H.K., Mirocha, C.J. & Gunther, R. Production of zearalenone, nivalenol, moniliformin, and wortmannin from toxigenic cultures ofFusarium obtained from pasture soil samples collected in New Zealand. Mycotox Res 7, 53–60 (1991). https://doi.org/10.1007/BF03192166

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  • DOI: https://doi.org/10.1007/BF03192166

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