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Determination and pharmacokinetics of danshensu in rat plasma after oral administration of danshen extract using liquid chromatography/tandem mass spectrometry

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Summary

A liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed and validated for the determination and pharmacokinetics of danshensu in rat plasma samples using ferulic acid as internal standard (IS). The plasma samples were treated by liquid-liquid extraction, and the analyses were determined using electrospray negative ionization mass spectrometry in selected reaction monitoring (SRM) mode. The signal intensity of them/z 196.8 → 134.8 transition of danshensu was found to relate linearly to danshensu concentrations in the plasma from 5–500 ng/mL. The lower limit of quantification (LLOQ) as determined by the LC/MS/MS method amounted to 5 ng/mL. The intra- and inter-day precision was below 10.82%, and the accuracy was between −3.51% and +11.92%. The validated LC/MS/MS method was applied to a pharmacokinetic study in which danshen extract (containing 40 mg/g danshensu) was administered orally to rats at a single dose of 200 mg/kg in 2% water.

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Authors and Affiliations

  1. School of Chemical Engineering and Technology, Tianjin University, Tianjin, Republic of China

    Wei Li & Chang-Xiao Liu

  2. Tasly R&D Institute, Tianjin Tasly Group Co., Ltd., Tianjin, People’s Republic of China

    Wei Li, Zhi-Wen Li, Jian-Ping. Han, Xin-Xin Li & Jun Gao

  3. Tianjin State Key Laboratory of Pharmacokinetics and Pharmacodynamics, Tianjin Institute of Pharmaceutical Research, Tianjin, People’s Republic of China

    Chang-Xiao Liu

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  1. Wei Li
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  2. Zhi-Wen Li
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  4. Xin-Xin Li
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Li, W., Li, ZW., Han, JP. et al. Determination and pharmacokinetics of danshensu in rat plasma after oral administration of danshen extract using liquid chromatography/tandem mass spectrometry. Eur. J. Drug Metabol. Pharmacokinet. 33, 9–16 (2008). https://doi.org/10.1007/BF03191013

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  • DOI: https://doi.org/10.1007/BF03191013

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