Summary
The antioxidant potential of a new flavonic derivative named conventionally theophylline-rutoside [TR-1722] was estimated by the measurement of G-6-Pase activity (marker enzyme for the endoplasmic reticulum), catalase activity (enzyme involved in the antioxidant defence process), and total-SH groups from the hepatic homogenate, using CCl4 as a free radical generating model. To show changes in the permeability of the hepatocyte membrane, the activity of lactate dehydrogenase (LDH) in plasma was determined. The obtained results suggest that TR-1722 acts by curtailing both lipid peroxidation and alkylation processes.
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References
Danila Gh., Nechifor M. (1990): Cercetari privind obtinerea prin semisinteza a unor derivati flavonici farmacologic activi. 1. Unele insusiri farmacologice ale Teofilin-Rutozidului (TR-1722). Rev. Med. Chir. Soc. Med. Nat. Iasi, 1, 151–156.
Comporti M. (1989): Three models of free radical induced cell injury. Chem. Biol. Interact., 72, 1–56.
De Duve C., et al. (1955): Tissue fractionation studies. Intracellular destruction patterns of enzymes in rat liver tissue. Biochem. J., 60, 606–617.
Harper E.A. (1965): Glucose-6-phosphatase determination in liver and kidney. In: Bergmeyer H.U. (Ed.). Methods of Enzymatic Analysis. Weinheim. Verlag Chemie, p. 788.
Worthington Enzymes Manual. (1972). New Jersey, Worthington Biochemical Corp., p. 41.
Bergmeyer H.U., Bernt E., Hess E. (1965): Lactic dehydrogenase methods of enzymatic analysis. Bergmeyer H.U. (Ed.) Methods of Enzymatic Analysis. Weinheim, Verlag Chemie, p. 736.
Sedlac J. (1968): Determination of total-SH groups. Anal. Biochem., 25, 192–195.
Lowry O.H., Rosenbrough H.J., Farr A.L., Randall R.J. (1951): Protein measurement with the Folin-phenol reagent. J. Biol. Chem., 193, 263–275.
Pessayre D., et al. (1982): Hepatotoxicity of trichlorethylene-carbon tetrachloride mixture in rats. Gastroenterology, 83, 761–772.
Camps J., Bargallo T., Gimenez A., et al. (1992): Relationship between hepatic lipid peroxidation and fibrogenesis in carbon tetrachloride-treated rats: effect of zinc administration. Clin. Sci., 83, 695–700.
Al. Arab M., Hamilton A.G. (1986): Possible model, reaction for some amine oxidases. Kinetics and mechanism of the copper (II) catalysed autooxidation of some diaminouracils. J. Am. Chem. Soc., 108, 5872–5878.
Timenstein M., Reed J.D. (1989): Effects of glutathione on the alfa-tocopherol-dependent inhibition of nuclear lipid peroxidation. J. Lipids, 30, 959–969.
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Paduraru, I., Saramet, A., Danila, G. et al. Antioxidant action of a new flavonic derivative in acute carbon tetrachloride intoxication. European Journal of Drug Metabolism and Pharmacokinetics 21, 1–6 (1996). https://doi.org/10.1007/BF03190270
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DOI: https://doi.org/10.1007/BF03190270