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Foreign gene transfer into Chinese shrimps (Penaeus chinensis) with gene gun

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Chinese Science Bulletin

Abstract

Plasmids pG DNA-RZ1 with aGFP (green fluorescent protein) reporter gene and a ribozyme gene incising penaeid white spot baculovirus (WSBV) were first introduced into the fertilized eggs of Chinese shrimps by gene gun. The treated and control samples of different development stages were observed with a fluorescent microscope. The transient expression ofGFP gene was high in nauplius and zoea larvae. Results from RT-PCR and PCR for adults showed that the foreign genes had been transferred into the shrimps and had expressed the corresponding proteins. This work has established a transgenic method for penaeid shrimps, which will set base for the application of genetic engineering breeding into industry.

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Authors and Affiliations

  1. Experimental Marine Biology Laboratory, Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China

    Zhiyi Liu, Jianhai Xiang & Guoying Zhou

  2. Shanghai Institute of Biochemistry, Chinese Academy of Sciences, 200031, Shanghai, China

    Zuxun Gong

Authors
  1. Zhiyi Liu
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  2. Jianhai Xiang
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  3. Guoying Zhou
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  4. Zuxun Gong
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Correspondence to Jianhai Xiang.

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Liu, Z., Xiang, J., Zhou, G. et al. Foreign gene transfer into Chinese shrimps (Penaeus chinensis) with gene gun. Chin.Sci.Bull. 46, 766–770 (2001). https://doi.org/10.1007/BF03187219

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  • DOI: https://doi.org/10.1007/BF03187219

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