Abstract
AFLP analysis of near-isogenic lines of the stripe rust resistance geneYr10 was carried out with 6Pst I-primers and 10Taq I -primers with the donor parent ofYr10 gene as the check. A total of about 4200 distinguishable bands were amplified, of which 5 were stable. The genetic linkage of the 5 polymorphic DNA fragments with the target gene were tested preliminarily on a segregating F2 population derived from a cross between the gene donor parent “More” and susceptible cultivar “Mingxian 169”. The DNA fragment PT0502 was found closely linked to theYr10 gene and cloned and sequenced. Based on the sequence specific primers for PCR were designed and synthesized. Genetic linkage analysis with 195 segregating F2 plants indicated that the genetic distance was 0.5 cM between the main product SC200 fragment produced by PCR with the primers and theYr10 gene. The primers can be used to detect theYr10 gene quickly, effectively and exactly.
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References
Mclniosh, R. A., Hart, G. E., Devos, K. M. et al., Catalogue of Gene Symbols for Wheat, Proceedings of the 9th International Wheat Genetics Symposium, Vol. 5, Saskatoon, Canada: University Extension Press, 1998, 235.
Rogers, S. O., Bendich, A. J., Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues, Plant Molecular Biology, 1985, 5: 69.
Vos, P., Hogcn, R., Blecker, M. et al., AFLP: a new technique for DNA fingerprinting, Nucl. Acids Res., 1995, 23(21); 4407.
Cho, Y. G., Blair, M. W., Panaud, O. et al., Cloning and mapping of variety-specific rice genomic DNA sequences: amplified fragment length polymorphism (AFLP) from silver-stained polyacrylamide gels, Genome, 1996, 39: 373.
Sun, G. L., Fahima, T., Korol, A. B. et al., Identification of mo-Jecular markers linked to theYr15 stripe rust resistant gene of wheat originated in wild emmer wheat, Theor. Appl. Genet., 1997, 95: 622.
Schachermayr, G. M., Messmer, M. M., Feuillet, V. et al., Identification of molecular markers linked toAgropyron elongaium-derived leaf rust resistant gene Lr24 in wheat, Theor. Appl. Genet., 1995, 30: 982.
Cadalen, T. C., Boecf, S., Bernard, S. et al., An intervarictal molecular marker map inTriticum aestivum L. Em. Thell. and comparison with a map from a wide cross, Theor. Appl. Genet., 1997, 94: 367.
Chao, S., Sharp, P. J., Worland, E. J. et al., RFLP-based genetic maps of wheat homologous group 7 chromosomes, Theor Appl. Genet., 1989, 78: 495.
Bennett, M. D., Smith, J. B., Nuclear DNA amounts in angiosperms, Phil. Trans. Roy. Soc. Lound. B, 1976, 274: 227.
Zabeau, M., Vos, P., Selective restriction fragment amplification: a general method for DNA fingerprinting, European Patent Application, Publication No. EP0534858, Paris: European Patent Office, 1993.
Ntu, Y. C., Liu, H. Y., Zhong, M. et al., RAPD markers linked to yellow rust resistance genes in wheat. The Ninth European Congress on Biotechnology CD ROM Proceedings (ed Hofman, M.), Brussels; Branche Belge de la Société de Chimie Industrielle, 1999, M2481X.
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Shao, Y., Niu, Y., Zhu, L. et al. Identification of an AFLP marker linked to the stripe rust resistance geneYr10 in wheat. Chin.Sci.Bull. 46, 1466–1468 (2001). https://doi.org/10.1007/BF03187033
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DOI: https://doi.org/10.1007/BF03187033