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Effect of UV-B radiation on growth, photosynthetic activity and metabolic activities ofChlorococcum sp.

  • Ecological and Environmental Microbiology
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Abstract

The impact of two intensities (2.5 W m−2 and 5 W m−2) of ultraviolet-B (UV-B) radiation on growth, photosynthetic pigments, photosynthetic activity and membrane leakage has been studied inChlorococcum sp. isolated from El-Kased fresh water canal, Tanta, Egypt. Exposure ofChlorococcum sp. for 60 and 35 min of 2.5 and 5 W m−2, respectively, inhibited growth by 50%. Chlorophylla and chlorophyllb were decreased inChlorococcum sp. by exposure to UV-B, but the effect was more pronounced on chlorophyllb. On the other hand, carotenoids were stimulated at small doses (time of exposure and intensity) of UV-B whether at 2.5 or 5 W m−2. However, oxygen amount, total soluble carbohydrates, and total soluble proteins were inhibited by UV-B treatment at all exposure times but the effect of 5 W m−2 was more than that of 2.5 W m−2 and O2 was completely abolished after 90 and 120 min at 2.5 and 5 W m−2, respectively. With regard to extracellular polysaccharides, there was a significant increase at small doses of UV-B but reduction observed at high doses. Exposing cells to 2.5 and 5 W m−2 UV-B caused 22.7 and 39.6% increase, respectively, in electrolyte leakage than in control.

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References

  • Aro E.M., Virgin I., Anderson B. (1993). Photoinhibition of photosystem II. Inactivation, protein damage and turnover. Biochim. Biophys. Acta, 1143: 113–134.

    Article  CAS  PubMed  Google Scholar 

  • Babu G.S., Joshi P.C., Viswanathan, P.N. (1998). UV-B-induced reduction in biomass and overall productivity of cyanobacteria. Biochem. Biophys. Res. Comm., 244: 138–142.

    Article  CAS  PubMed  Google Scholar 

  • Babu T.S., Jansen M.A.K., Greenberg B.M., Gaba V., Malkin S., Mattoo A.K., Edelman M. (1999). Amplified degradation of photosystem II D1 and D2 proteins under a mixture of photosynthetically active radiation and UVB radiation: Dependence on redox status of photosystem II. Photochem. Photobiol., 69: 553–559.

    Article  CAS  Google Scholar 

  • Bancroft B.A., Baker N.J., Blaustein A.R. (2007). Effects of UVB radiation on marine and freshwater organisms: a synthesis through meta-analysis. Ecol. Letters, 10(4): 332–345.

    Article  Google Scholar 

  • Bhargava P., Atri N., Srivastava A.K., Rai L.C. (2007). Cadmium mitigates ultraviolet-B stress inAnabaena doliolum: Enzymatic and non-enzymatic antioxidants. Biol. Plantarum, 51 (3): 546–550.

    Article  CAS  Google Scholar 

  • Bornman J.F., Sundby-Emanuelsson C. (1995). Response of plants to UV-B radiation: Some biochemical and physiological effects. In: Smirnoff N., Ed., Environmental and Plant Metabolism: Flexibility and Acclimation, Bios Scientific, Oxford, pp. 245–262.

    Google Scholar 

  • Correia C.M., Pereira J.M.M., Coutinho J.F., Björn L.O., Torres-Pereira J.M.G. (2005). Ultraviolet-B radiation and nitrogen affect the photosynthesis of maize: a mediaterranean field study. Eur. J. Agron., 22: 337–347.

    Article  CAS  Google Scholar 

  • Ehling-Schulz M., Bilger, W., Scherer S. (1997). UV-B-induced synthesis of photoprotective pigments and extracellular polysaccharides in the terrestrial cyanobacteriumNostoc commune. J. Bacteriol., 1940–1945.

  • El-Shintinawy F. (2000). Photosynthesis in two wheat cultivars differing in salt susceptibility. Photosynthetica, 38 (4): 615–620.

    Article  CAS  Google Scholar 

  • Estevez M.S., Malanga G., Puntarulo S. (2001). UV-B effects on AntarcticChlorella sp. cells. J. Photochem. Photobiol. B: Biol., 62: 19–25.

    Article  CAS  Google Scholar 

  • Figueroa F.L., Nygård C., Ekelund N., Gómez I. (2003). Photobiological characteristics and photosynthetic UV responses in twoUlva species (Chlorophyta) from southern Spain. J. Photochem. Photobiol. B: Biol., 72: 35–44.

    Article  CAS  Google Scholar 

  • Garde K. (1998). Effects of ultraviolet-B radiation on marine plankton organisms, and the impact on the structure and function of pelagic ecosystems. Ph.D. Thesis, Copenhagen University, Fresh Water Biological Laboratory.

  • Gerloff G.G., Fitzgerald G.P., Skooge F. (1950). The isolation, purification and culture of blue-green algae. Amer. J. Bot., 37: 216–218.

    Article  CAS  Google Scholar 

  • Hideg É., Vass I. (1996). UV-B induced free radical production in plant leaves and isolated thylakoid membranes. Plant Sci., 115: 251–260.

    Article  CAS  Google Scholar 

  • Jeffrey S.W., Humphrey G.F. (1975). New spectrophotometric equations for determining chlorophyll a, b, c1 and c2 in higher plants, algae and natural phytoplankton. Biochem. Physiol. Pflanz., 167: 191–194.

    CAS  Google Scholar 

  • Jensen A., Liaan Jensen S. (1959). Qualitative paper chromatography of carotenoids. Acta Chem. Scand., 13: 1863–1868.

    Article  CAS  Google Scholar 

  • Kaplan D., Christiaen D., Arad S. (1987). Chelating properties of extracellular polysaccharides fromChlorella sp. Appl. Environ. Microbiol. 53 (12): 2953–2956.

    CAS  PubMed  Google Scholar 

  • Kochert G. (1973). Carbohydrate determination by the Phenol-Sulphoric acid method. In: Handbook of Phycological and Biochemical Methods. Cambridge University Press, Cambridge.

    Google Scholar 

  • Kuhl A. (1962). Zur physiologic der speicherung organis cher phosphate inChlorella. Biet. Physiol. Morphol. Algen. Gustav. Fresh Verlag. Stuttgart, Germany, pp. 157.

    Google Scholar 

  • Kumar A., Tyagi M.B., Singh N., Tyagi R., Jha P.N., R.P., Häder D.-P. (2003). Role of white light in reversing UV-B-mediated effects in the N2-fixing cyanobacteriumAnabaena BT2. J. Photochem. Photobiol. B: Biol., 71: 35–42.

    Article  CAS  Google Scholar 

  • Lowry O.M., Rosebrough N.J., Farr L.A., Randall R. J. (1951). Protein measurements with the Folin phenol reagent. J. Biol. Chem., 193: 265–275.

    CAS  PubMed  Google Scholar 

  • Mackerness S.A.H., Surplus S.L., Jordan B.R., Thomas B. (1997). Ultraviolet-B effects on transcript levels for photosynthetic genes are not mediated through carbohydrate metabolism. Plant Cell Environ., 20: 1431–1437.

    Article  CAS  Google Scholar 

  • Madronich S., McKenzie R.L., Caldwell M.M., Björn L.O. (1995). Changes in ultraviolet radiation reaching the earth’s surface. Ambio, 24: 143–152.

    Google Scholar 

  • Malanga G., Calmanovici G., Puntarulo S. (1997). Oxidative damage to chloroplasts fromChlorella vulgaris exposed to ultraviolet B-radiation. Plant Physiol., 101: 455–462.

    Article  CAS  Google Scholar 

  • Melis A., Nemson J.A., Harrison M.A. (1992). Damage to functional components and partial degradation of photosystem II reaction centre proteins upon chloroplast exposure to ultraviolet-B radiation. Biochim. Biophys. Acta, 1100: 312–320.

    CAS  Google Scholar 

  • Mishra S., Srivastava S., Tripathi R.D., Govindarajan R., Kuriakose S.V. (2006). Phytochelatin synthesis and response of antioxidants during cadmium stress inBacopa monnieri L. Plant Physiol. Biochem., 44 (1): 25–37.

    Article  CAS  PubMed  Google Scholar 

  • Payne J.K., Stewart J.R. (1988). The chemical composition of the thallus wall ofCharaciosophon rivularis (Charaiosiphonaceae, Chlorophyta). Phycology, 27 (1): 43–49.

    CAS  Google Scholar 

  • Post A., Larkum A. W. D. (1993). UV-absorbing pigments, photosynthesis and UV exposure in Antarctica: comparison of terrestrial and marine algae. Aquat. Bot., 45: 231–243.

    Article  Google Scholar 

  • Prather M., Midgley P., Rowland F.S., Stolarski R.S. (1996). The ozone layer: the road not taken. Nature, 381: 551–554.

    Article  CAS  Google Scholar 

  • Prescott G.W. (1975). Algae of the Western Great Lakes Area. Department of Botany and Pathology, Michigan State Uni. East Lansing, Michigan.

    Google Scholar 

  • Rai L.C., Tyagi B., Mallick N., Rai P.K. (1995). Interactive effects of UV-B and copper on photosynthetic activity of the cyanobacteriumAnabaena doliolum. Environ. Exp. Bot., 35 (2): 177–185.

    Article  CAS  Google Scholar 

  • Shi S., Wang G., Wang Y., Zhang L., Zhang L. (2005). Protective effect if nitric oxide against oxidative stress under ultraviolet-B radiation. Nitric Oxide, 13: 1–9.

    Article  CAS  PubMed  Google Scholar 

  • Shick J.M. (1993). Solar UV and oxidative, stress in algal-animal symbioses. In: Shima A., Ichihashi M., Fujiwara Y., Takebe H., Eds, Frontiers of, Photobiology, Excerpta Medica, Amsterdam, pp. 561–564.

    Google Scholar 

  • Shick J.M., Dunlap W.C. (2002). Mycosporine like amino acids and related gadusols: Biosynthesis, accumulation, and UV-protective functions in aquatic organisms. Annu. Rev. Physiol., 64: 223–262.

    Article  CAS  PubMed  Google Scholar 

  • Sinha R.P., Häder D.-P. (2000) Effects of UV-B radiation on cyanobacteria, Recent Res. Devel. Photochem. Photobiol., 4: 239–246.

    Google Scholar 

  • Sinha R.P., Singh N., Kumar A., Kumar H.D., Häder D.-P. (1997). Impacts of ultraviolet-B irradiation on nitrogen-fixing cyanobacteria of rice paddy fields. J. Plant Physiol., 150: 188–193.

    CAS  Google Scholar 

  • Teramura A.H., Sullivan J.H. (1994). Effects of UV-B radiation on photosynthesis and growth of terrestrial plants. Photosynth. Res., 39: 463–473.

    Article  CAS  Google Scholar 

  • Tyagi M.B., Kumar A., Kumar H.D. (1993). Effects of ultraviolet-B radiation on photosynthetic14CO2 uptake and oxygen amount in cyanobacteria. Phykos, 32 (1, 2): 175–180.

    Google Scholar 

  • van Hasselt P.R., Chow W.S., Anderson J.M. (1996). Short-term treatment of pea leaves with supplementary UV-B at different oxygen concentrations: Impacts on chloroplast and plasma membrane bound processes. Plant Sci., 120: 1–9.

    Article  Google Scholar 

  • Vani B., Saradhi P. P., Mohanty P. (2001) Alteration in chloroplast structure and thylakoid membrane composition due toin vivo heat treatment of rice seedlings: Correlation with the functional changes. J. Plant Physiol., 158: 583–592.

    Article  CAS  Google Scholar 

  • Vasiliokiotis C., Melis A. (1994). Photosystem II reaction center damage and repair cycle: Chloroplast acclimation strategy to irradiance stress. Proc. Nat. Acad. Sci. USA, 91: 7222–7226.

    Article  Google Scholar 

  • Venkataraman G.S. (1969). The Cultivation of Algae. Indian Council of Agricultural Research, New Delhi, India.

    Google Scholar 

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Authors and Affiliations

  1. Phycology Research Unit, Botany Department, Faculty of Science, Tanta University, Tanta, Egypt

    Atef Mohamed Abo-Shady, Mostafa Mohamed El-Sheekh, Amal Hamed El-Naggar & Abd El-Fatah Abomohra

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  1. Atef Mohamed Abo-Shady
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  2. Mostafa Mohamed El-Sheekh
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  3. Amal Hamed El-Naggar
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  4. Abd El-Fatah Abomohra
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Correspondence to Atef Mohamed Abo-Shady.

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Abo-Shady, A.M., El-Sheekh, M.M., El-Naggar, A.H. et al. Effect of UV-B radiation on growth, photosynthetic activity and metabolic activities ofChlorococcum sp.. Ann. Microbiol. 58, 21–27 (2008). https://doi.org/10.1007/BF03179440

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