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Identification ofAcetobacter strains isolated in Thailand based on 16S–23S rRNA gene ITS restriction and 16S rRNA gene sequence analyses

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Abstract

Ninety-seven acetic acid bacteria, which were isolated from fruits, flowers and other material collected in Thailand by an enrichment culture approach, were assigned to the genusAcetobacter by phenotypic and chemotaxonomic characterisations. On the basis of 16S–23S rRNA gene ITS restriction and 16S rRNA gene sequence analyses, the ninety-seven isolates were grouped into three groups and identified at the specific level: 1) group A including fifty-three isolates, which were identified asAcetobacter pasteurianus, 2) group B including forty-two isolates, which were identified asAcetobacter orientalis and 3) group C including two isolates, which were identified asAcetobacter lovaniensis. There was no isolate to be assigned to other 15 species of the genusAcetobacter.

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Author notes

  1. Yuzo Yamada (JICA Senior Overseas Volunteer, Professor Emeritus)

    Present address: Japan International Cooperation Agency (JICA), Tokyo, Japan

Authors and Affiliations

  1. Department of Microbiology, Faculty of Science, Chulalongkorn University, 10330, Bangkok, Thailand

    Jintana Kommanee & Ancharida Akaracharanya

  2. Department of Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, 10330, Bangkok, Thailand

    Somboon Tanasupawat

  3. BIOTEC Culture Collection (BCC), BIOTEC Central Research Unit, National Centre for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, 12120, Pathumthani, Thailand

    Taweesak Malimas, Pattaraporn Yukphan & Yuzo Yamada (JICA Senior Overseas Volunteer, Professor Emeritus)

  4. NITE Biological Resource Centre (NBRC), Department of Biotechnology, National Institute of Technology and Evaluation (NITE), 292-0818, Kisarazu, Japan

    Yasuyoshi Nakagawa

Authors
  1. Jintana Kommanee
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  2. Ancharida Akaracharanya
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  3. Somboon Tanasupawat
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  4. Taweesak Malimas
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  5. Pattaraporn Yukphan
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  6. Yasuyoshi Nakagawa
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  7. Yuzo Yamada
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Correspondence to Ancharida Akaracharanya.

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Kommanee, J., Akaracharanya, A., Tanasupawat, S. et al. Identification ofAcetobacter strains isolated in Thailand based on 16S–23S rRNA gene ITS restriction and 16S rRNA gene sequence analyses. Ann. Microbiol. 58, 319–324 (2008). https://doi.org/10.1007/BF03175337

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  • DOI: https://doi.org/10.1007/BF03175337

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