Abstract
Phylogenetic analysis of about twenty isolates ofShigella spp. was carried out using the nucleotide sequences of 16S rRNA, 23S rRNA and DNA gyrase B (gyrB) genes which was determined by directly sequencing PCR fragments. The results showed that the similarity based on 16S rRNA for specimens belonging to the same species ofShigella flexneri, Shigella boydii, Shigella dysenteriae andShigella sonnei were 99.7, 99.6, 99.5 and 100%, the corresponding results based on 23S rRNA were 99.4, 99.7, 99.3 and 100%, and forgyrB were 99.4, 99.7, 99.1 and 100% respectively. Our results demonstrate thatgyrB sequence analysis is a fruitful approach to determine the phylogenetic relationships ofShigella isolates and may be an alternative to 16S rRNA analysis. In particular,gyrB analysis of bacteria is an effective means to classify closely related species, while 23S rRNA gene sequence data may be used for phylogenetic analysis at the species level.
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Nochi, Z., Sahebekhtiari, N., Kharaziha, P. et al. Comparison of 16S rRNA, 23S rRNA andgyrB genes sequences in phylogenetic relationships ofShigella isolates from Iran. Ann. Microbiol. 59, 615–622 (2009). https://doi.org/10.1007/BF03175154
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DOI: https://doi.org/10.1007/BF03175154