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Identification of bacteria producing a thermophilic lipase with positional non-specificity and characterization of the lipase

  • Industrial Microbiology
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Abstract

A bacterial isolate producing lipase, named S31, was isolated from soil and identified as a strain ofBurkholderia cepacia. S31 produced high activity of lipase which reached a maximum of 226.1 u/ml by fermenting at 30 °C for 60 h under the induction of olive oil. After purification, the lipase showed a single band of about 35 kDa in SDS-PAGE. The optimum temperature of the lipase was 70 °C and the optimum pH was 9.0. S31 lipase was stable at 40-70 °C and pH 0.5–10.0, as well as in several organic solvents, such as methanol, n-hexane, n-butanol, toluene and ethyl acetate. The presence of some metal ions (Ca2+, Mn2+, K+, Na+ and Mg2+) could activate the enzyme whereas Fe2+ and Cu2+ were found to be inhibitory. The lipase could cleave all of the three ester bonds of triglycerides. We conclude that S31 lipase is an alkaline lipase with a variety of highly desirable characteristics for research and industrial application.

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Authors and Affiliations

  1. College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, People’s Republic of China

    Yaping Lu

  2. College of Food Science and Technology, Nanjing Agricultural University, 210095, Nanjing, People’s Republic of China

    Fengxia Lu, Xiang Wang, Xiaomei Bie, Huigang Sun,  Wuyundalai & Zhaoxin Lu

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  1. Yaping Lu
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  2. Fengxia Lu
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  3. Xiang Wang
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  4. Xiaomei Bie
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  5. Huigang Sun
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  6. Wuyundalai
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  7. Zhaoxin Lu
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Correspondence to Zhaoxin Lu.

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Lu, Y., Lu, F., Wang, X. et al. Identification of bacteria producing a thermophilic lipase with positional non-specificity and characterization of the lipase. Ann. Microbiol. 59, 565–571 (2009). https://doi.org/10.1007/BF03175147

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  • DOI: https://doi.org/10.1007/BF03175147

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