Abstract
We investigated the occurrence ofTrichoderma in the Oueslatia forest located in the centre-west of Tunisia, which represents a good example for studying a distribution ofTrichoderma between different soil horizons of a forest with Mediterranean bioclimate. One hundred-five isolates obtained were identified at the genus level by analysis of morphological characters. The diversity ofTrichoderma between soil horizons was evaluated by analysis of the internal transcribed spacer (ITS) of the rDNA region using restriction fragment length polymorphism (RFLP). The ITS region was first amplified by polymerase chain reaction (PCR) with specific primers and then cleaved with two restriction enzymes. Amplification products showed extensive length polymorphism and RFLP analysis generated bands ranging from 100 to 620 bp. The restriction profiles of the PCR products showed thatHaeIII enzyme has more discriminatory power thanRsaI. A high degree of polymorphism was detected in rhizosphere rather than soil. Thus the RFLP analysis produced different DNA profiles on the gels denoting significant intraspecific genetic variation. Our data confirm the potential of ITS region PCR-RFLP for the study of polymorphism amongTrichoderma population from different soil layers.
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References
Ahmad J.S., Baker R. (1988). Implications of rhizosphere competence ofTrichoderma harzianum. Can. J. Microbiol., 34: 229–234.
Bisset J. (1991). A revision of the genusTrichoderma. III. SectionPachybasium. Can. J. Bot., 69: 2373–2417.
Cook, R.J., Baker K.F. (1983). The soil ecosystem. In: The Nature and Practice of Biological Control of Plant Pathogens. Am. Phytopathol. Soc. Press, St. Paul, MN, pp. 233–282.
Dal Soglioa F.K.,. Bertagnollib B.L.,. Sinclairb J.B., Yub G.-Y., Eastburn D.M. (1998). Production of chitinolytic enzymes and endoglucanase in the soybean rhizosphere in the presence ofTrichoderma harzianum andRhizoctonia solani. Biol. Control, 12 (2): 111–117.
Doyle J.J., Doyle J.L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull., 19: 11–15.
Emberger L. (1960). In: L’exploitation Traditionnelle du Maquis du Nord de la Tunisie: Possibilite d’une Meilleure utilisation. OEP-Ministere de l’Agriculture Tunisienne, GTZ, Eschborn, République Fédérale d’Allemagne.
Harman G.E. (2000). Myths and dogmas of biocontrol changes in perceptions derived from research onTrichoderma harzianum T22. Plant Dis., 84: 377–393.
Harman G.E., Petzoldt R., Comis A., Chet J. (2004). Interactions betweenTrichoderma harzianum strain T22 and maize inbred line Mo17 and effects of this interaction on diseases caused byPythium ultimum andColletotrichum graminicola. Phytopathology, 94: 147–153.
Hjeljord L., Transmo, A. (1998).Trichoderma andGlicoladium in biological control: an overview. In: Harman G.E., Kubicek C.P., Eds, Biological Control and Commercial Applications, Taylor & Francis, London, UK, pp. 131–151.
Kubicek C.P., Penttilä M.E. (1998). Regulation of production of plant polysaccharide degrading enzymes byTrichoderma. In: Harman G.E., Kubicek C.P., Eds, Biological Control and Commercial Applications, Taylor & Francis, London, UK, pp. 49–71.
Lieckfeldt E., Khuls K., Muthumeenakshi S. (1998). Molecular taxonomy ofTrichoderma and Glicoladium and their teleomorphs. In: Kubicek C.P., Harman G.E., Eds,Trichoderma andGlicoladium. Basic Biology, Taxonomy and Genetics, Vol 1, Taylor and Francis, London, pp. 35–37.
Mtimet A. (2001). Soils of Tunisia. In: Zdruli P., Steduto P., Lacirignola C., Montanarella L., Eds, Soil Resources of Southern and Eastern Mediterranean Countries. Options Méditerranéennes: Série B. Etudes et Recherches; n. 34, Bari: CIHEAM-IAMB, pp. 243–262.
Nei M., Li W.H. (1979). Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci., USA, 76: 5269–5273.
Nelson E.E. (1982). Occurrence ofTrichoderma in a Douglas-fir soil. Mycologia, 74: 280–284.
Papavizas G.C. (1985).Trichoderma andGlicoladium: biology and potential for biological control. Ann. Rev. Phytopathol., 23: 23–54.
Rifai M.A. (1969). A revision of the genusTrichoderma. Mycol. Papers, 116: 1–56.
Samuels G.J. (1996).Trichoderma: a review of biology and systematics of the genus. Mycol. Res., 100: 923–935.
Sivasithamparam K., Ghisalberti E.L. (1998). Secondary metabolism inTrichoderma andGlicoladium. In: Kubicek C.P., Harman G.E., Eds,Trichoderma andGlicoladium. Basic Biology, Taxonomy And Genetics, Taylor & Francis, London, UK, pp. 139–191.
Thrane C., Tronsmo A., Jensen D.N. (1997). Glucanase and cellulase fromTrichoderma harzianum: purification and partial chracarterization, induction and biological activity against plant pathogenicPyhtium spp. Eur. J. Plant Pathol., 103: 331–344.
Widden P., Abitbol J.J. (1980). Seasonality ofTrichoderma species in spruce-forest soils. Mycologia, 72: 775–784.
White T., Bruns T., Lee S., Taylor J. (1990). Analysis of phylogenetic relationships by amplification and direct sequencing of ribosomal RNA genes for phylogenetics. In: Innis M.A., Gelfand D.H., Sninsky J.J., White T.J., Eds, PCR Protocols: A Guide to Methods and Applications, Academic Press, New York, pp. 315–322.
Wuczkowski M., Druzhinina I., Gherbaway Y., Klug B., Prillinger H., Kubicek C.P. (2003). Species pattern and genetic diversity ofTrichoderma in a mid-European, primeval floodplain-forest. Microbiol. Res., 158: 125–133.
Yedidia I., Benhamou N., Chet I. (1999). Induction of defence responses in cucumber plants (Cucumis sativus L.) by the biocontrol agentTrichoderma harzianum. Appl. Environ. Microbiol., 653: 1061–1070.
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Sadfi-Zouaoui, N., Châabani, S., Rouaissi, M. et al. Analysis of the diversity ofTrichoderma spp. in soil horizons using digested ITS regions. Ann. Microbiol. 59, 459–463 (2009). https://doi.org/10.1007/BF03175131
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DOI: https://doi.org/10.1007/BF03175131