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Antibacterial and cytotoxic activities of extracts from (Tunisian)Rhamnus alaternus (Rhamnaceae)

  • Applied Microbiology
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Abstract

The petroleum ether, chloroformic, ethyl acetate, methanolic, Total Oligomers Flavonoids (TOF) enriched extracts, water extract as well as its fractions A1, A2, A3 obtained from aerial parts ofRhamnus alaternus, a Tunisian-Mediterranean medicinal species, were investigated for the contents of phenolic compounds, cytotoxic activity against the K562 human chronic myelogenous leukaemia cell line and L1210 leukaemia murine cells and for antibacterial activity against Gram positive and Gram negative bacterial reference strains. A pronounced cytotoxic effect on both the cell lines was shown in the TOF, ethyl acetate, methanolic, aqueous extracts and A2 fraction, with respectively IC50 values 75, 232, 298, 606 and 571 μg/ml on K562 cells and 198, 176, 767, 560 and 614 μg/ml on L1210 cell line. Significant activity against bacterial reference strains:Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Salmonella enteritidis andSalmonella typhimurium was shown with ethyl acetate, TOF extracts and A2 fraction. The antimicrobial and cytotoxic activities showed byR. alatemus depended on the chemical composition of the tested extracts.

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References

  • Abdelwahed A., Bouhlel I., Skandrani I., Valenti K., Kadri M., Guiraud P., Steiman R., Mariotte A.M., Ghedira K., Laporte F., Dijoux-Franca M.G., Chekir-Ghedira L. (2007). Study of antimutagenic and antioxidant activities of gallic acid and 1,2,3,4,6-pentagalloylglucose fromPistacia lentiscus, Confirmation by microarray expression profiling. Chem. Biol. Inter., 165: 1–13.

    Article  CAS  Google Scholar 

  • Abou-Chaar Ch.I., Kabbara R.A., Shamlian N.S. (1982). A chromatographic study of the anthraquinones ofRhamnus alaternus L. Extraction, isolation and chromatographic characterization of the anthraglycosides of the stern bark. Int. J. Crude Drug. Res., 20 (1): 13–18.

    CAS  Google Scholar 

  • Ahn Y.J., Park S.J., Lee, S.G., Shin S.C., Choi D.H. (2000). Cordycepin: selective growth inhibitor derived from liquid culture ofCordyceps militaris againstClostridium spp. J. Agric. Food Chem., 48: 2744–2748.

    Article  PubMed  CAS  Google Scholar 

  • Al-Dabbas M.M., Suganuma T., Kitahara K., Hou D.-X., Fujii M. (2006). Cytotoxic, antioxidant and antibacterial activities ofVarthemia iphionoides Boiss. extracts. J. Ethnopharmacol., 108: 287–293.

    Article  PubMed  Google Scholar 

  • Becx M.C.J.M., Janssen A.J.H.M., Claaener H.A.L., de Koning R.W. (1990). Metronidazole-resistantHelicobacter pylori. Lancet, 335: 539–540.

    Article  PubMed  CAS  Google Scholar 

  • Bellakhdar J. Ed. (1999). La Pharmacopée Marocaine Traditionnelle. Ibis Press, Paris.

    Google Scholar 

  • Ben Ammar R., Kilani S., Abdelwahed A., Hayder N., Mahmoud A., Chibani J., Chekir-Ghedira L., Ghedira K. (2005).In vitro mutagenicity, antimutagenicity and free radical scavenging activities ofRhamnus alaternus L. (Rhamnaceae) extracts. Pak. J. Biol. Sci., 8 (3): 439–445.

    Article  Google Scholar 

  • Ben Mansour H., Boubaker J., Bouhlel I., Mahmoud A., Bernillon S., Ben Chibani J., Ghedira K., Chekir-Ghedira L. (2007). Antigenotoxic activities of crude extracts fromAcacia salicina leaves. Env. Mol. Mutagen., 48: 1–9.

    Article  CAS  Google Scholar 

  • Ben Mrabet K., Abed L. (1986). Quelques aspects de la pharmacopée traditionnelle algérienne. Pharmacien du Maghreb, spécial 2: 18–282.

    Google Scholar 

  • Benavente-Garcia O., Casillo O., Marin F., Ortuno A., Del-Rio J. (1997). Uses and properties ofCitrus flavonoids. J. Agric. Food Chem., 45: 4505–4515.

    Article  CAS  Google Scholar 

  • Bouhlel I., Ben Mansour H., Limem I., Ben Sghaier M., Mahmoud A., Ben Chibani J., Ghedira K., Chekir-Ghedira L. (2007). Screening of antimutagenicity via antioxidant activity in different extracts from the leaves ofAcacia salicina from the center of Tunisia. Environ. Toxicol. Pharmacol., 23: 56–63.

    Article  CAS  Google Scholar 

  • Boukef K. (2001).Rhamnus alaternus. Essaydali, 81: 34–35.

    Google Scholar 

  • Capecka E., Mareczek A., Leja M. (2005). Antioxidant activity of fresh and dry herbs of some Lamiaceae species. Food Chem., 93: 223–226.

    Article  CAS  Google Scholar 

  • Chevolleau S., Debal A., Ucciani E. (1992). Détermination de l’activité antioxydante d’extraits végétaux. Rev. Fr. Corps Gras, 39 (1/2): 3–8.

    CAS  Google Scholar 

  • Chiang L.C., Chiang W., Liu M.C., Lin C.C. (2003).In vitro antiviral activities ofCaesalpinia pulcherrima and its related flavonoids. J. Antimicrob. Chemother., 52: 194–198.

    Article  PubMed  CAS  Google Scholar 

  • Conner D.E. (1993). Naturally occurring compounds. In: Davidson P., Branen A.L., Eds, Antimicrobials in Foods, Marcel Dekker Inc., New York, pp. 441–468.

    Google Scholar 

  • Cragg G.M., Boyd M.R., Cardellina II J.H., Newman D.J., Snader K.M., McCloud T.G. (1994). Ethanobotany and drug discover: the experience of US National Cancer Institute. Ethanobotany and Search for New Drugs, Ciba Foundation Symposium 185. Wiley, Chichester, pp. 178–196.

    Book  Google Scholar 

  • Cremieux A. (1991). Perspectives méthodologiques dans l’évaluation de l’activité antibactérienne des huiles essentielles. In: Phytothérapie, Aromathérapie, Encycl. Med. Nat., Paris, C-4.

  • Dhidah M., Trabelsi A., Mzoughi R., Dhidah L., Boujaafar N., Jeddi M. (1995).Salmonella and salmonelloses in the region of Sousse (Tunisia). Microbiol. Hyg. Alim., 7 (19): 3–7.

    Google Scholar 

  • Dorman H.J.D., Deans S.G. (2000). Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J. Appl. Microbiol., 88: 308–316.

    Article  PubMed  CAS  Google Scholar 

  • Duh P.-D., Yen G.C. (1997). Antioxidative activity of three water extracts. Food Chem., 60: 639–645.

    Article  CAS  Google Scholar 

  • Eberhardt M.V., Lee C.Y., Liu R.H. (2000). Antioxidant activity of fresh apples. Nature, 405: 903–904.

    PubMed  CAS  Google Scholar 

  • Efthimiadou E.K., Thomadaki H., Sanakis Y., Raptopoulou C.P., Katsaros N., Scorilas A., Karaliota A., Psomas G. (2007). Structure and biological properties of the copper (II) complex with the quinolone antibacterial drug N-propyl-norfloxacin and 2,2′-bipyridine. J. Inorg. Biochem., 101 (1): 64–73.

    Article  PubMed  CAS  Google Scholar 

  • Eloff J.N. (1998). A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta Med., 64: 711–713.

    Article  PubMed  CAS  Google Scholar 

  • Hayder N., Ben Ammar R., Abdelwahed A., Kilani S., Mahmoud A., Ben Chibani J., Mariotte A.M., Ghedira K., Dijoux-Franca M.G., Chekir-Ghedira L. (2005). Antibacterial and antimutagenic activity of extracts and essential oil from (Tunisian)Pistacia lentiscus. Toxicol. Env. Chem., 87 (1–4): 567–573.

    Article  CAS  Google Scholar 

  • Hirano T., Gotoh M., Oka K. (1994). Natural flavonoids and lignans are potent cytostatic agents against human leukemic HL-60 cells. Life Sci., 55: 1061–1069.

    Article  PubMed  CAS  Google Scholar 

  • Ismail N.H., Ali A.M., Aimi N., Kitajima M., Takayama H., Lajis N.H. (1997). Anthraquinones fromMorinda elliptica. Phytochemistry, 45 (8): 1723–1725.

    Article  CAS  Google Scholar 

  • Izhaki I., Tsahar E., Paluy I., Friedman J. (2002). Within population variation and interrelationships between morphology, nutritional content and secondary compounds ofRhamnus alaternus fruits. New Phytol., 156: 217–223.

    Article  CAS  Google Scholar 

  • Jemal A., Murray T., Samuels A., Ghafoor A., Ward E., Thun M. (2003). Cancer statistics, CA Cancer J. Clin., 53: 5–26.

    Article  PubMed  Google Scholar 

  • Kim M.S., Lee J., Lee K.M., Yang S.H., Choi S., Chung S.Y., Kim T.Y., Jeong W.H., Park R. (2003). Involvement of hydrogen peroxide in mistletoe lectin-II-induced apoptosis of myeloleukemic U937 cells. J. Life Sci., 73: 1231–1243.

    Article  CAS  Google Scholar 

  • Kumar A., Chattopadhyay S. (2007). DNA damage protecting activity and antioxidant_potential of pudina extract. Food Chem., 100: 1377–1384.

    Article  CAS  Google Scholar 

  • Kuntz S., Wenzel U., Daniel H. (1999). Comparative analysis of the effects of flavonoids on proliferation, cytotoxicity, and apoptosis in human colon cancer cell lines. Eur. J. Nutr., 38 (3): 133–142.

    Article  PubMed  CAS  Google Scholar 

  • Larocca L.M., Piantelli M., Leone G., Sica S., Teofili L., Benedetti-Panici P. Scambia G., Mancuso S., Capelli A., Ranelletti F. (1990). Type II oestrogen binding sites in acute lymphoid and myeloid leukemias: growth inhibitory effect of oestrogen and flavonoids. Br. J. Haematol., 75: 489–495.

    Article  PubMed  CAS  Google Scholar 

  • Lee R.M., Hirano K.I., Anant S., Baunoch D., Davidson N.O. (1998). An alternatively spliced form of apobec-1 messenger RNA is over expressed in human colon cancer. Gastroenterology, 115 (5): 1096–1103.

    Article  PubMed  CAS  Google Scholar 

  • Luximon-Ramma A., Bahorun T., Soobrattee M.A., Aruoma O. (2002). Antioxidant activities of phenolic, proanthocyanidin, and flavonoid components in extract ofCassia fistula. J. Agric. Food Chem., 50 (18): 5042–5047.

    Article  PubMed  CAS  Google Scholar 

  • Marzouk M.S., El-Toumy S.A.A., Merfort I., Nawwar M.A.M. (1999). Polyphenolic metabolites, ofRhamnus disperma. Phytochemistry, 52 (5): 943–946.

    Article  CAS  Google Scholar 

  • Matsumoto S., Washizuka Y., Matsumoto Y., Tawara S., Ikeda F., Yokota Y., Karita M. (1997). Appearance of a metronidazole-resistantHelicobacter pylori strain in an infected-ICR-mouse model and difference in eradication of metronidazole-resistant and- sensitive strains. Antimicrob. Ag. Chemother., 41: 2602–2605.

    CAS  Google Scholar 

  • Mead P.S., Slutsker L., Dietz V., McCaig L.F., Bresee J.S., Shapiro C., Griffin P.M., Tauxe R.V. (1999). Food-related illness and death in the United States. Emerg. Inf. Dis., 5: 607–625.

    Article  CAS  Google Scholar 

  • Musonda C.A., Chipman J.K. (1998). Quercetin inhibits hydrogen peroxide (H2O2)-induced NF-kappa B DNA binding activity and DNA damage in HepG2 cells. Carcinogenesis, 19: 1583–1589.

    Article  PubMed  CAS  Google Scholar 

  • Nwabueze T.U. (2007). Effect of process variables on trypsin inhibitor activity (TIA), phytic acid and tannin content of extruded African bread fruit-corn-soy mixtures: A response surface analysis, LWT-Food Sci. Technol., 40: 21–29.

    CAS  Google Scholar 

  • O’Brien S.J., de Valk H. (2003).Salmonella — “old” organism, continued challenges! Eurosurveillance, 8: 29–31.

    Google Scholar 

  • Park B.S., Lee H.K., Lee S.E., Piao X.L., Takeoka G.R., Wong R.Y., Ahna Y.J., Kima J.H. (2006). Antibacterial activity ofTabebuia impetiginosa Martius ex DC (Taheebo) againstHelicobacter pylori. J. Ethnopharmacol., 105: 255–262.

    Article  PubMed  CAS  Google Scholar 

  • Pearson D. (1976). The Chemical Analysis of Foods, 7th edn. Churchill Livingstone, London.

    Google Scholar 

  • Polydoro M., De Souza K.C.B., Andrades M.E., Da Silva E.G., Bonatto F., Heydrich J., Dal-Pizzol F., Schapoval E.E.S., Bassani V.L., Moreira J.C.F. (2004). Antioxidant, a pro-oxidant and cytotoxic effects ofAchyrocline satureioides extracts. Life Sci., 74: 2815–2826.

    Article  PubMed  CAS  Google Scholar 

  • Pottier-Alapetite G. (1978). Flore de la Tunisie, Imprimerie Officielle de la République Tunisienne, pp. 138–141.

  • Rodriguez Vaquero M.J., Alberto M.R., Manca de Nadra M.C. (2007). Antibacterial effect of phenolic compounds from different wines. Food Control, 18 (2): 93–101.

    Article  CAS  Google Scholar 

  • Rosell G. (1980). Aspectos farmacognósticos y farmacodinámicos deRhamnus alaternus ssp.myrtifolia Will. II, Cir. Farm., 38 (269): 445–458.

    CAS  Google Scholar 

  • Roussis V., Tsoukatou M., Chinou I., Ortiz A. (1998). Composition and antibacterial activity of the essential oils ofHelichrysum rupestre andHelichrysum ambiguum growing in the Balnearic islands. Planta Med., 64: 675–676.

    Article  PubMed  CAS  Google Scholar 

  • Sakanaka S., Juneja L.R., Taniguchi M. (2000). Antimicrobial effects of green tea polyphenols on thermophilic spore-forming bacteria. J. Biosci. Bioeng., 90 (1): 81–85.

    PubMed  CAS  Google Scholar 

  • Todd E.C.D. (1989). Costs of acute bacterial foodborne disease in Canada and the United States. Int. J. Food Microbiol., 9 (4): 313–326.

    Article  PubMed  CAS  Google Scholar 

  • Tona L., Cimanga R.K., Mesia K., Musuamba C.T., De Bruyne T., Apers S., Hernans N., Von Miert S., Pieters L., Totté J., Vlietinck A.J. (2004).In vitro antiplasmodial activity of extracts and fractions from seven medicinal plants used in Democratic Republic of Congo. J. Ethnopharmacol., 93: 27–32.

    Article  PubMed  CAS  Google Scholar 

  • Tona L., Kambu K., Ngimbi N., Cimanga K., Vlietnick A.J. (1998). Antiamoebic and phytochemical screening of some Congolese medicinal plants. J. Ethnopharmacol., 61: 57–65.

    Article  PubMed  CAS  Google Scholar 

  • Uliasz T.F., Hewett S.J. (2000). A microtiter trypan blue absorbance assay for the quantitative determination of excytotoxic neuronal injury in cell culture, J. Neurosc. Meth., 100: 157–163.

    Article  CAS  Google Scholar 

  • Wink M. (1993). Production and application of phytochemicals from an agricultural perspective. In: van Beek T.A., Breteler H., Eds., Phytochemistry and Agriculture, Proc. Phytochem. Soc. Europe, Vol. 34, Clarendon, Oxford University, Press, pp. 171–213.

    Google Scholar 

  • Yuan V.Y., Bone D.E., Carrington F. (2005). Antioxidant activity of dulse (Palmaria palmata) extract evaluatedin vitro. Food Chem., 91 (3): 485–494.

    Article  CAS  Google Scholar 

  • Zhishen J., Mengcheng T., Jianming W. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem., 64 (4): 555–559.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Unité de Pharmacognosie/Biologie Moléculaire 99/UR/07-03, Faculté de Pharmacie de Monastir, Rue Avicenne, 5019, Monastir, Tunisie

    Rebai Ben Ammar, Soumaya Kilani, Ines Bouhlel, Ines Skandrani, Aicha Naffeti, Jihed Boubaker, Mohamed Ben Sghaier, Wissem Bhouri, Amor Mahmoud, Leila Chekir-Ghedira & Kamel Ghedira

  2. Faculté de Médecine Dentaire de Monastir, Laboratoire de Biologie Moléculaire et Cellulaire, Rue Avicenne, 5019, Monastir, Tunisie

    Leila Chekir-Ghedira

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  1. Rebai Ben Ammar
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  2. Soumaya Kilani
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  3. Ines Bouhlel
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  4. Ines Skandrani
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  10. Leila Chekir-Ghedira
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  11. Kamel Ghedira
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Correspondence to Leila Chekir-Ghedira.

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Ben Ammar, R., Kilani, S., Bouhlel, I. et al. Antibacterial and cytotoxic activities of extracts from (Tunisian)Rhamnus alaternus (Rhamnaceae). Ann. Microbiol. 57, 453–460 (2007). https://doi.org/10.1007/BF03175089

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  • DOI: https://doi.org/10.1007/BF03175089

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