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Pro-antioxidant ratio in healthy men exposed to muscle-damaging resistance exercise

Estado oxidativo en varones sanos sometidos a ejercicio contra resistencia

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Abstract

The purpose of this study was to compare the pro-antioxidant status in healthy men exposed to muscle-damaging resistance exercise, and to investigate the practical application of Loverro’s coefficient (P/A ratio) to evaluate the presence of oxidative stress. Twenty-eight healthy men were assigned to two groups performed multi-joint (M) or single-joint (S) resistance exercise. The activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) as well as the concentration of lipid peroxidation products (TBARS) in blood were evaluated. The P/A ratio was calculated from the mean values of erythrocyte TBARS, SOD, CAT and GPx. Creatine kinase (CK) activity was used as a marker of muscle damage. The applied resistance exercises triggered off the changes in pro-antioxidant ratio towards peroxidation which was proved by significant increase in erythrocyte TBARS concentration in M (+25%) and S (+27%) groups. Plasma TBARS increased only after multi-joint resistance exercise and correlated with erythrocyte P/A ratio (r=0.536, P<0.01). The multi-joint exercise caused decrease in SOD activity by 28% whereas the single-joint resistance exercise elevated enzyme activity by 20%. Activities of the other antioxidant enzymes changed simultaneously i.e. CAT activity increased by 14%–16% immediately after exercise, and GPx activity declined by 18%–34% during recovery in M and S groups. Even though, all erythrocyte parameters significantly changed following multi-joint and single-joint resistance exercises, the assessment of proantioxidant ratio showed the considerable increase in P/A only in M group. In summary, an analysis of pro- and antioxidant parameters showed significant changes in response to muscle-damaging exercise and demonstrated the practical application of P/A ratio to evaluate the risk of oxidative stress in athletes.

Resumen

Se estudia en el presente trabajo el estado oxidativo en hombres sanos sometidos a ejercicio contra resistencia con lesión muscular y la aplicación práctica del coeficiente de Loverro (relación P/A) para evaluar la existencia de estrés oxidativo. El estudio se llevó a cabo con 28 hombres sanos divididos en 2 grupos según realizaran ejercicio contra resistencia multiarticular (grupo M) o de articulación simple (grupo S). Se determinaron en los eritrocitos las actividades de superóxido dismutasa (SOD), glutatión peroxidasa (GPx) y catalasa (CAT) y la concentración de productos de peroxidación lipídica (TBARS). La relación de prooxidantes/antioxidantes (relación P/A) se calculó a partir de los valores medios de TBARS/SOD, CAT y GPx. La actividad creatina quinasa se utilizó como marcador de lesión muscular. El ejercicio contra resistencia dió lugar a incremento de la concentración de TBARS en eritrocitos tanto en el grupo M (+25%) como en el S (+27%). En plasma, el nivel de TBARS aumentó sólo en el grupo M, en correlación con la relación P/A (r=0.536; P<0.01). En el grupo M, la actividad SOD disminuyó un 28%, aumentando un 20% en el grupo S. La actividad de los otros enzimas antioxidantes se modificó paralelamente en los dos grupos (la de CAT, aumentó immediatamente después del ejercicio y la de GPx disminuyó durante la recuperación). En conjunto, los resultados indican cambios significativos en los parámetros pro y antioxidantes en respuesta al ejercicio con lesión muscular y demuestran la aplicación práctica de la relación P/A para evaluar el riesgo de estrés oxidative en los atletas.

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Authors and Affiliations

  1. Department of Biochemistry and Sport Medicine, Faculty of Physical Culture Gorzow Wlkp., University of Physical Education Poznan, Poland

    A. Zembron-Lacny, J. Ostapiuk & K. Szyszka

  2. Department of Sport Medicine, University of Physical Education Wroclaw, Poland

    M. Slowinska-Lisowska

  3. Department of Theory and Methodics of Sports Disciplines, University of Physical Education Wroclaw, Poland

    K. Witkowski

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  1. A. Zembron-Lacny
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  2. J. Ostapiuk
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  3. M. Slowinska-Lisowska
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  4. K. Witkowski
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  5. K. Szyszka
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Correspondence to A. Zembron-Lacny.

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Zembron-Lacny, A., Ostapiuk, J., Slowinska-Lisowska, M. et al. Pro-antioxidant ratio in healthy men exposed to muscle-damaging resistance exercise. J. Physiol. Biochem. 64, 27–35 (2008). https://doi.org/10.1007/BF03168232

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  • DOI: https://doi.org/10.1007/BF03168232

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