Skip to main content
SpringerLink
Log in

Bone specific binding sites for 1,25(OH)2D3 in magnesium deficiency

Unión específica de 1,25(OH)2D3 a hueso en la deficiencia en magnesio

  • Published:
Journal of Physiology and Biochemistry Aims and scope Submit manuscript

Abstract

It has been reported that some hypoparathyroid patients with magnesium deficiency showed altered responses to vitamin D treatment. In the same way,in vitro bone studies have demonstrated the existence of a decrease in the 1,25-dihydroxyvitamin D3-induced resorption in bone as a result of magnesium deficiency. These findings suggest some kind of alteration in the 1,25(OH)2D3 in bone in magnesium deficiency. In the present work, using a binding assay based on the 1,25(OH)2D3 and3H-1,25(OH)2D3 competition for the hormone binding sites in rat calvaria homogenates, a significant decrease in the number of 1,25(OH)2D3 specific binding sites has been found in calvaria incubated in magnesium-deficient medium compared to magnesium-replete ones. Alterations in the hormone-receptor affinity were not found. These results suggest that an alteration in the 1,25(OH)2D3 action on magnesium-deficient bone could be due, at least in part, to a decrease in the number of available vitamin D receptors in bone cells.

Resumen

Se ha descrito que algunos pacientes hipoparatiroideos deficientes en magnesio mostraban una respuesta alterada al tratamiento con la vitamina D. Además, en estudiosin vitro con cultivos óseos deficientes en magnesio, se ha observado descenso en los procesos de resorción del hueso inducida por la 1,25-dihidroxi-vitamina D3 (1,25(OH)2D3), sugiriendo una posible alteración sobre las acciones de la 1,25(OH)2D3 en los huesos deficientes en magnesio. En este trabajo, se ha encontrado un descenso significativo en el número de sitios de unión de la 1,25(OH)2D3 en calotas deficientes en magnesio frente a calotas repletas en Mg2+, mediante un ensayo de competición entre la hormona fría y la hormona marcada radioactivamente. Sin embargo, no se encontraron alteraciones en la afinidad de la hormona por su receptor. Estos resultados sugieren que las alteraciones observadas en la acción de la 1,25(OH)2D3 en huesos deficientes en magnesio podrían ser debidas, al menos en parte, a un descenso en el número de receptores disponibles para la 1,25(OH)2D3 en células óseas.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Biggers, J. D., Gwatkin, R. B. and Heyner, S. (1961):Exp. Cell. Res.,25, 41–58.

    Article  CAS  PubMed  Google Scholar 

  2. Darwish, H. M. and DeLuca, H. F. (1996):Prog. Nucleic Acid. Res. Mol. Biol.,53, 321–344.

    Article  CAS  PubMed  Google Scholar 

  3. Ducreaux, M., Messing, B., De Vernejoul, M. C., Buhnik, Y., Miravet, L. and Rambaud, J. C. (1991):Gastroenterol. Clin. Biol.,15, 805–811.

    Google Scholar 

  4. Freake, H. C. and McCarthy, D. M. (1984):Cancer Res.,44, 3627–3631.

    CAS  PubMed  Google Scholar 

  5. Johannesson, A. J. and Raisz, L. G. (1983):Endocrinology,113, 2294–2298.

    Article  CAS  PubMed  Google Scholar 

  6. Lemay, J. and Gascon-Barré, M. (1992):Endocrinology,130, 2767–2777.

    Article  CAS  PubMed  Google Scholar 

  7. Lifshitz, F., Harrison, H. C. and Harrison, H. E. (1967):Proc. Soc. Exp. Biol. Med.,125, 472–476.

    CAS  PubMed  Google Scholar 

  8. Medalle, R., Waterhouse, C. and Hahn, T. J. (1976):Am. J. Clin. Nutr.,29, 854–858.

    CAS  PubMed  Google Scholar 

  9. Norman, A. W., Roth, J. and Orci, L. (1982):Endocrinol. Rev.,3, 331–366.

    Article  CAS  Google Scholar 

  10. Municio, M. J., Traba, M. L. and De la Piedra, C. (1995):Horm. Metab. Res.,27, 296–299.

    Article  CAS  PubMed  Google Scholar 

  11. Pike, J. W. (1991):Ann. Rev. Nutr.,11, 189–216.

    Article  CAS  Google Scholar 

  12. Pan, L. C. and Price, P. A. (1984):J. Biol. Chem.,259, 5844–5847.

    CAS  PubMed  Google Scholar 

  13. Risco, F. and Traba, M. L. (1992):Magnesium Res.,5, 5–14.

    CAS  Google Scholar 

  14. Rosler, A. and Rabinowitz, D. (1973):Lancet,1, 803–805.

    Article  CAS  PubMed  Google Scholar 

  15. Rude, R. K. and Singer, F. (1981):Ann. Rev. Med.,32, 245–259.

    Article  CAS  PubMed  Google Scholar 

  16. Rude, R. K., Adams, J. S., Ryzen, E., Endress, M., Niimi, H., Horst, R. L., Haddad, J. G. and Singer, F. R. (1985):J. Clin. End. Metab.,61, 933–936.

    Article  CAS  Google Scholar 

  17. Rude, R. K., Gruber, H. E., Norton, H. J. and Wei, Y. (2004):J. Nutr.,134, 79–85.

    CAS  PubMed  Google Scholar 

  18. Saggese, G., Federico, G., Bertelloni, S., Baroncelli, G. I. and Calisti, L. (1991):J. Pediatry,118, 220–225.

    Article  CAS  Google Scholar 

  19. Traba, M. L., De la Piedra, C. and Rapado, A. (1989):Med. Sci. Res.,17, 327–328.

    CAS  Google Scholar 

  20. Weaver, V. M. and Welsh, J. (1993):J. Nutr.,123, 764–771.

    CAS  PubMed  Google Scholar 

  21. Wecksler, W. R. and Norman, A. W. (1980):J. Biol. Chem.,255, 3571–3578.

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratorio de Bioquímica, Sección de Fisiopatología Ósea, Fundación Jiménez Díaz, Avda Reyes Católicos 2, 28040, Madrid, Spain

    F. Risco & M. L. Traba

Authors
  1. F. Risco
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. L. Traba
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Risco, F., Traba, M.L. Bone specific binding sites for 1,25(OH)2D3 in magnesium deficiency. J. Physiol. Biochem. 60, 199–203 (2004). https://doi.org/10.1007/BF03167029

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03167029

Key words

Palabras clave

Search

Navigation