Abstract
Uricase catalyzes the oxidation of urate to form allantoin, carbon dioxide and hydrogen peroxide. In this article, we demonstrate for the first time the presence of a radical intermediate involved in the reaction mechanism. Such radical species was entrapped using 5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide as spin trap and the relative adduct was detected by electron paramagnetic resonance (EPR) technique. A structure of such radical (5-hydroperoxy isourate) is proposed, through chemical results and density functional theory calculations of the EPR coupling constants.
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Busi, E., Sinicropi, A., Terzuoli, L. et al. Identification and structural characterization of a transient radical species in the uricase reaction mechanism. Appl. Magn. Reson. 31, 471–482 (2007). https://doi.org/10.1007/BF03166597
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DOI: https://doi.org/10.1007/BF03166597