Abstract
A method of selective hole burning in EPR spectra was applied to determine the distances from a radical to the acceptor quinone-iron in bacterial and plant photosystems. A low amplitude hole burning 180° pulse and high amplitude 90° and 90° pulses applied to detect ESE of P870+ inRb. Sphaeroides and the distance from the primary electron donor P870+ to the acceptor Q −A Fe2+ was determined to be 26±2 Å from the dipolar broadening of the burned hole in P870+ EPR. This result is consistent with that given by X-ray analysis and susceptibility measurement. In plant photosystem II the same method was applied to the EPR spectrum of tyrosine D+, but the effect of crystalline field splitting of Fe2+ ion was taken into consideration. The effective spin value for the ferrous iron in PS II was found to be 0.8 and the distance between the radical and the non-heme iron was obtained to be 42±2 Å.
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Hara, H., Kawamori, A. A selective hole burning method applied to determine distances between paramagnetic species in photosystems. Appl. Magn. Reson. 13, 241–257 (1997). https://doi.org/10.1007/BF03161984
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DOI: https://doi.org/10.1007/BF03161984