Abstract
Thirty and 60-min ischemic insults resulted in an increase in free fatty acid and 1,2- diacylglycerol contents of rat forebrain. No significant changes were detected in phospholipids except phosphatidylinositol 4-monophosphate and phosphatidylinositol 4,5-bisphosphate during ischemic insult. Phosphatidylinositol 4-monohosphate and phosphatidylinositol 4,5-bisphosphate contents decreased during ischemia. Although the increase in free fatty acid contents continued, 1,2-diacylglycerol did not show further increase after 30-min ischemia. These results suggest that there may be another pathway for the accumulation of free fatty acids in addition to phospholipase C coupled to di- and monoacylglycerol lipase.
Free fatty acid and 1,2-diacylglycerol contents increased transiently and thereafter decreased to control levels within 90 min after postischemic recirculation. The decrease, in arachidonic acid content preceded those of other FFA. Phosphatidylinositol 4-monophosphate and phosphatidylinositol 4,5-bisphosphate contents gradually increased after the initiation of recirculation in ischemic brains. Lysophosphatidylcholine decreased gradually after temporary increase during 15 and 5-min recirculations in 30 and 60-min ischemic Phospholipase A, phospholipase C, and di- and mono-acylglycerol lipase activities did not show significant changes during entire course of recirculation. Total activities of lysophospholipase and acylation enzymes of lysophospholipid demonstrated 1.5-and 2.2-fold increase during 30-min recirculation.
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Abbreviations
- PC:
-
phosphatidylcholine
- PE:
-
Phosphatidylethanolamine
- PS:
-
Phosphatidylserine
- PI:
-
Phosphatidylinositol
- SM:
-
Sphingomyelin
- PIP:
-
Phosphatidylinositol 4-monophosphate
- PIP2 :
-
Phosphatidylinositol 4,5-bisphosphate
- LysoPC:
-
Lysophosphatidylcholine
- LysoPE:
-
Lysophosphatidylethanolamine
- PLA:
-
Phospholipase A
- LPL:
-
Lysophospholipase
- PLC:
-
Phospholipase C
- DG+MG lipase:
-
Di and Mono-acylglycerol lipase
- 1,2-DG:
-
1,2-Diacylglycerol
- FFA:
-
Free fatty acid
- GroPCho:
-
sn-Glycero-3-phosphocholine
- GroPIns:
-
sn-Glycero-3-phosphoinositol
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Moto, A., Hirashima, Y., Endo, S. et al. Changes in lipid metabolites and enzymes in rat brain due to ischemia and recirculation. Molecular and Chemical Neuropathology 14, 35–51 (1991). https://doi.org/10.1007/BF03160996
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DOI: https://doi.org/10.1007/BF03160996