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Characterization of fenarimol-resistant mutants of Aspergillus nidulans

  • Resistance to Systemic Fungicides
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Netherlands Journal of Plant Pathology Aims and scope Submit manuscript

Abstract

The fitness of twelve fenarimol-resistant mutants of Aspergillus nidulans was tested with respect to spore germination, germ tube elongation, hyphal growth and sporulation. Half of the strains tested were isolated on triarimolcontaining media. The other strains were selected on imazalil-or cycloheximide-containing media (Van Tuyl, 1977).

A number of mutant strains produced spores with unimpaired viability on synthetic medium. In other strains a reduction in spore viability was noticed. The rate of germ tube elongation of all resistant mutants was significantly lower than that of the wild-type strain. Mutant strains with a low degree of resistance always had an almost normal mycelial growth rate, whereas growth of some of the strains with a relatively high degree of resistance was significantly slower. Spore production on malt agar was highest in the wild-type strain and was found to be lower in fenarimol-resistant mutants. In most of the mutant strains tested a high degree of cross-resistance between fenarimol, imazalil and triadimefon was established; in some of them cross-resistance to these chemicals was low or even absent.

Possible implications of the results described for the chance of development of resistance in phytopathogenic fungi to sterol biosynthesis-inhibiting fungicides are discussed.

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References

  • Brown, I.F., Taylor, H.M. & Hall, H.R., 1975. EL-222, a new fungicide with eradicative activity. Proc. Am. phytopath. Soc. 2: 31.

    Google Scholar 

  • Buchenauer, H., 1975. Systemisch-fungizide Wirkung und Wirkungsmechanismus von Triadimefon (MEB 6447). Mitt. biol. BundAnst. Ld- u. Forstw. 165: 154–155.

    Google Scholar 

  • Clutterbuck, A.J., 1974. Aspergillus nidulans. In: R.C. King (Ed.), Handbook of Genetics, Vol. I: Bacteria, bacteriophages and fungi. Plenum Press, New York, p. 447–510.

    Google Scholar 

  • Dekker, J., 1976. Acquired resistance to fungicides. A. Rev. Phytopath. 14: 405–428.

    Article  CAS  Google Scholar 

  • Fuchs, A. & Drandarevski, C.A., 1976. The likelihood of development of resistance to sýstemic fungicides which inhibit ergosterol biosynthesis. Neth. J. Pl. Path. 82: 85–87.

    Article  CAS  Google Scholar 

  • Fuchs, A. & Viets-Verweij, M., 1975. Permanent and transient resistance to triarimol and triforine in some phytopathogenic fungi. Meded. Fac. Landbouww. Rijksuniv. Gent 40: 699–706.

    Google Scholar 

  • Fryberg, M., Oehlschlager, A.C. & Unrau, A.M., 1974. Sterol biosynthesis in antibiotic-resistant yeast: nystatin. Archs Biochem. Biophys. 160: 83–89.

    Article  CAS  Google Scholar 

  • Hamilton-Miller, J.M.T., 1974. Fungal sterols and the mode of action of polyene antibiotics. Adv. appl. Microbiol. 17: 109–134.

    Article  PubMed  CAS  Google Scholar 

  • Ragsdale, N.N., 1975. Specific effects of triarimol on sterol biosynthesis in Ustilago maydis. Biochim. biophys. Acta 380: 81–96.

    PubMed  CAS  Google Scholar 

  • Ragsdale, N.N. & Sisler, H.D., 1973. Mode of action of triarimol in Ustilago maydis. Pestic. Biochem. Physiol. 3: 20–29.

    Article  CAS  Google Scholar 

  • Rank, G.H., Robertson, A. & Phillips, K., 1975. Reduced plasma membrane permeability in a multiple cross resistant strain of Saccharomyces cerevisiae. J. Bact. 122: 359–366.

    PubMed  CAS  Google Scholar 

  • Rosenberger, R.F. & Kessel, M., 1967. Synchrony of nuclear replication in individual hyphae of Aspergillus nidulans. J. Bact. 94: 1464–1469.

    PubMed  CAS  Google Scholar 

  • Sherald, J.L., Ragsdale, N.N. & Sisler, H.D., 1973. Similarities between the systemic fungicides triforine and triarimol. Pestic. Sci. 4: 719–727.

    Article  CAS  Google Scholar 

  • Siegel, M.R., Kerkenaar, A. & Kaars Sijpesteijn, A., 1977. Antifungal studies with the systemic fungicide imazalil. Neth. J. Pl. Path. 83S: 121–133.

    Article  Google Scholar 

  • Tuyl, J.M. van, 1977. Genetic aspects of resistance to imazalil in Aspergillus nidulans. Neth. J. Pl. Path. 83S: 169–176.

    Google Scholar 

  • Waard, M.A. de & Sisler, H.D., 1976. Resistance to fenarimol in Aspergillus nidulans. Meded. Fac. Landbouww. Rijksuniv. Gent 41: 571–578.

    Google Scholar 

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Authors and Affiliations

  1. Laboratory of Phytopathology, Agricultural University, Wageningen, The Netherlands

    M. A. de Waard & S. A. Gieskes

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  1. M. A. de Waard
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  2. S. A. Gieskes
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de Waard, M.A., Gieskes, S.A. Characterization of fenarimol-resistant mutants of Aspergillus nidulans. Neth. J. Pl. Path. 83 (Suppl 1), 177–188 (1977). https://doi.org/10.1007/BF03041433

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  • DOI: https://doi.org/10.1007/BF03041433

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