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Expression of CD34 in gastric cancer and its correlation with histology, stage, proliferation activity, p53 expression and apoptotic index

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Pathology Oncology Research

Abstract

The formation of new blood vessels is essential for tumor growth and progression. Until today there are only few studies of the immunohistochemical assessment of angiogenesis in gastric cancer by the evaluation of the expression of CD34 antigen. The aim of this study was to analyze the relationship between microvessel density (MVD) expressed as the mean count of CD34 immunostained vessels and clinicopathologic features of gastric tumors (the histological type according to the Lauren classification, tumor grade - G; presence of lymph node metastases - N; depth of tumor invasion; stage of disease (UICC-AJCC 1988–1992), p53 expression, tumor cell proliferative activity described as the Ki67 labelling index and apoptotic index of tumor cells - TUNEL method). We assessed formalin-fixed, paraffin-embedded tissue samples obtained during potentially radical gastrectomy from 58 patients with primary gastric adenocarcinoma. The representative tissue blocks from each tumor were used for the immunohistochemical assay and examined by two pathologists independently. MVD was counted in five tumor areas of the most intensive neovascularization (x 200 field by light microscopy) and the mean counts were recorded. The mean MVD (CD34 expression value ± SD) in this study was 43,15 ± 19,8 per x 200 field. The study demonstrated the statistically significant correlation between MVD and two main histological parameters: tumor grading (p < 0.001) and tumor histological type according to Lauren’s classification (p<0.05). In well - and moderately - differentiated tumors (G1/2) MVD was significantly lower in comparison to the group of poorly differentiated cancer - G3 (mean value: 31,62 vs. 49,89). MVD was higher in diffuse type of gastric cancer comparing to intestinal type (50.05 ± 19,03 vs. 39.17 ±20,09). However, the authors failed to find a significant correlation between MVD and other investigated histopathological features in malignant gastric tumors. The close relationship between CD34 immunostaining, gastric cancer tumor vascularity and main histological parameters was shown in this study. It can be stated that analysis of expression of angiogenesis in gastric cancer may be helpful for better estimation of hematogenous recurrence and the selection of the group of patients for adjuvant antiangiogenic treatment.

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Authors and Affiliations

  1. Department of Oncological Surgery, Medical University of Lodz, Poland

    Micha Tenderenda

  2. Department of Soft Tissue/Bone Tumors, M. Sklodowska-Curie Memorial Cancer Center-Institute, Roentgena Str. 5, 02-781, Warsaw, Poland

    Piotr Rutkowski

  3. Department of Tumor Pathology, Chair of Oncology, Medical University of Lodz, Poland

    Dorota Jesionek-Kupnicka & Robert Kubiak

Authors
  1. Micha Tenderenda
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  2. Piotr Rutkowski
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  3. Dorota Jesionek-Kupnicka
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  4. Robert Kubiak
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Corresponding author

Correspondence to Piotr Rutkowski.

Additional information

This study was supported by the grant No 502-11-397 from Medical University of Lodz, Poland

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Tenderenda, M., Rutkowski, P., Jesionek-Kupnicka, D. et al. Expression of CD34 in gastric cancer and its correlation with histology, stage, proliferation activity, p53 expression and apoptotic index. Pathol. Oncol. Res. 7, 129–134 (2001). https://doi.org/10.1007/BF03032579

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  • DOI: https://doi.org/10.1007/BF03032579

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