Abstract
Light affects plant growth and development throughout the life cycle. However, light signals do not function autonomously but should be integrated with endogenous developmental factors such as the plant hormone auxin to specify correct developmental decisions. We have previously reported that theArabidopsis shy2-1D mutation alters various light responses, including highly photomorphogenic development in darkness. Here we show that the mutation also alters various auxin responses, including constitutive formation of lateral roots and reduced auxin sensitivity in inhibition of hypocotyl and root growth. The mutation is a gain of function mutation occuring in theIAA3 gene, one of theAux/IAA family genes encoding putative transcription factors of auxin-responsive genes. These results suggest that IAA3/SHY2 may play important roles in both light-and auxin-mediated development Considering that Aux/IAA proteins and auxin response transcription factors interact with one another, we propose that IAA3/SHY2 may integrate light signals into auxin-mediated developmental responses.
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Literature Cited
Abel S, Nguyen MD, Theologis A (1995) ThePS-IAA4/5- like family of early auxin-inducible mRNAs inArabidopsis thaliana. J Mol Biol 251: 533–549
Barnes SA, McGrath RB, Chua N-H (1997) Light signal transduction in plants. Trends Cell Biol 7: 21–26
Bechtold N, Pelletier G (1998)In planta Agrobacterium-mediated transformation of adultArabidopsis thaliana plants by vacuum infiltration. Methods Mol Biol 82: 259–266
Behringer FJ, Davies PJ (1997) lndole-3-acetic acid levels after phytochrome-mediated changes in the stem elongation rate of dark-and light-grownPisum seedlings. Planta 188: 85–92
Boerjan W, Cervera MT, Delarue M, Beeckmen T, Dewitte W, Bellini C, Caboche M, Van Onckelen H, Van Montagu M, Inze D (1995)superroot, a recessive mutation inArabidopsis, confers auxin overproduction. Plant Cell 7: 1405–1419
Castle LA, Meinke DW (1994) AFUSCA gene ofArabidopsis encodes a novel protein essential for plant development. Plant Cell 6: 25–41
Chang SC, Lee MS, Lee SM, Kim J, Kang BG (1994) Ethyl-ene-lnduced Auxin Sensitivity Changes in Petiole Epinasty of Tomato Mutantdgt. J Plant Biol 37: 257–262
Chory J, Chatterjee M, Cook RK, Elich T, Fankhauser C, Li J, Nagpal R, Neff M, Pepper A, Poole D, Reed J, Vitart V (1996) From seed germination to flowering, light controls plant development via the pigment phytochrome. Proc Natl Acad Sci USA 93: 12066–12071
Chory J, Li J (1997) Gibberellins, brassinosteroids and light-regulated development. Plant Cell Env 20: 801–806
Chory J, Reinecke D, Sim S, Washburn T, Brenner M (1994) A role for cytokinin in de-etiolation inArabidopsis. Plant Physiol 104: 339–347
Davis PJ (1995) Plant hormones and their roles in plant growth and development. Dordrecht, Kluwer Academic Publishers
Deng XW, Matsui M, Wei N, Wagner D, Chu AM, Feldmann KA, Quail PH (1992)COP1, anArabidopsis regulatory gene, encodes a protein with both a zinc-binding motif and a Gβ homologous domain. Cell 71: 791–801
Estelle M (1996) Plant tropism: The ins and outs of auxin. Curr Biol 6: 1589–1591
Fujita H, and Syono K (1997) PIS1, a negative regulator of the action of auxin transport inhibitors inArabidopsis thaliana. Plant J 12:583–595
Furuya M (1993) Phytochromes: their molecular species, gene families, and functions. Annu Rev Plant Physiol Plant Mol Biol 44: 617–645
Furuya M, Schäfer E (1996) Photoperception and signaling of induction reactions by different phytochromes. Trends Plant Sci 1: 301–307
Guilfoyle TJ (1998) Aux/IAA proteins and auxin signal transduction. Trends Plant Sci 3: 205–207 Jensen PJ, Hangarter RP, Estelle M (1998) Auxin transport is required for hypocotyl elongation in light-grown but not dark-grownArabidopsis. Plant Physiol 116: 455–462
Kaufman PB, Wu LL, Brock TG, Kim D (1995) Hormones and the orientation of growth,In PJ Davis, ed, Plant hormones and their roles in plant growth and development, Dordrecht, Kluwer Acadimic Publishers, pp 547–571
Kendrick RE, Kronenberg GHM (1994) Photomorphogene-sis in plants. Dordrecht, Kluwer Academic Publishers
Kim BC, Soh MS, Hong SH, Furuya M, Nam HG (1998) Photomorphogenic development of theArabidopsis shy2-lD mutation and its interaction with phytochromes in darkness. Plant J 15: 61–68
Kim BC, Soh MS, Kang BJ, Furuya M, Nam HG (1996) Two dominant photomorphogenic mutations ofArabidopsis thaliana identified as suppressor mutations ofhy2. Plant J 9: 441–456
Kim J, Harter K, Theologis A (1997) Protein-protein interactions among the Aux/IAA proteins. Proc Natl Acad Sci USA 94: 11786–11791
Kim SY, Mulkey TJ (1997a) Effect of Auxin and Ethylene on Elongation of Intact Primary Root of Maize (Zea mays L). J Plant Biol 40: 249–257
Kim SY, Mulkey TJ (1997b) Effect of Ethylene Antagonists on Auxin-induced Inhibition of Intact Primary Root Elongation in Maize (Zea mays L.). J Plant Biol 40: 256–260
Koornneef M, Rolff E, Spruit CJP (1980) Genetic control of light-inhibited hypocotyl elongation inArabidopsis thaliana (L.) Heynh. Z pflanzenphysiol 100: 147–160
Kraepiel Y, Marrec K, Sotta B, Caboche M, Miginiac E (1995)In vitro morphogenic characteristics of phytochrome mutants inNicotiana plumbaginifolia are modified and correlated to high indole-3-acetic acid levels. Planta 197: 142–146
Kraepiel Y, Miginiac E (1997) Photomorphogenesis and phytohormones. Plant Cell Env 20: 807–812
Li J, Nagpal P, Vitart V, McMorris TC, Chory J (1996) A role for brassinosteroids in light-dependent development ofArabidopsis. Science 272: 398–401
Luschnig C, Gaxiola RA, Grisafi PL, Fink GR (1998) EIR1, a root-specific protein involved in auxin transport, is required for gravitropism inArabidopsis thaliana. Genes Dev 12: 2175–2187
Nagatani A, Reed JW, Chory J (1993) Isolation and initial characterization ofArabidopsis mutants that are deficient in phytochrome A. Plant Physiol 102: 269–277
Ni M, Tepperman JM, Quail PH (1998) PIF3, a phyto-chrome-interacting factor necessary for normal photoinduced signal transduction, is a novel basic helix-loop-helix protein. Cell 95: 657–67
Nick P, Schafer E, Furuya M (1992) Auxin redistribution during first positive phototropism in corn coleoptile. Microtubule reorientation and the Cholodny-Went theory. Plant Physiol 99: 1302–1308
Parks BM, Quail PH (1993)hy8, a new class ofArabidopsis long hypocotyl mutants deficient in functional phytochrome A. Plant Cell 5: 39–48
Peng J, Harberd N (1997) Gibberellin deficiency and response mutations suppress the stem elongation phenotype of phytochrome-deficient mutants ofArabidopsis. Plant Physiol 113: 1051–1058
Pepper A, Delaney T, Washburn T, Poole D, Chory J (1994)DET1, a negative regulator of light-mediated development and gene expression inArabidopsis, encodes a novel nuclear-localized protein. Cell 78: 109–116
Quail PH, Boylan MT, Parks BM, Short TW, Xu Y, Wagner D (1995) Phytochromes: photosensory perception and signal transduction. Science 268: 675–680
Robson PRH, Smith H (1996) Genetic and transgenic evidence that phytochromes A and B act to modulate the gravitropic orientation ofArabidopsis thaliana hypocotyls. Plant Physiol 110: 211–216
Rouse D, Mackay P, Strinberg P, Estelle M, Ottoline L (1998) Changes in auxin response from mutations in anAUX/IAA gene. Science 279: 1371–1373
Smith H (1994) Sensing the light environment: the functions of the phytochrome family,In RE Kendrick, GHM Kronenberg, eds, Photomorphogenesis in Plants, 2nd edn, Dordrecht, Kluwer Academic Publishers, pp 377–416
Su W, Howell SH (1995) The effects of cytokinin and light on hypocotyl elongation inArabidopsis seedlings are independent and additive. Plant Physiol 108: 1423–1430
Tian Q, Reed JW (1999) Control of auxin-regulated root development by theArabidopsis thaliana SHY2/IAA3 gene. Development 126: 711–21.
Trewavas AJ (1992) What remains for the Cholodny-Went theory? Plant Cell Environ 15: 759–794
Ulmasov T, Hagen G, Guilfoyle TJ (1997a) ARF1, a transcription factor that binds to auxin responsive elements. Science 276: 1865–1868
Ulmasov T, Murfett J, Hagen G, Guilfoyle TJ (1997b) Aux/ IAA proteins repress expression of reporter genes containing natural and highly active synthetic auxin response elements. Plant Cell 9: 1963–1971
Usmanov PD, Sokhibnazarov SH (1975) Genetic and somatic effects of N-nitroso-methylbiuret onArabidopsis thaliana (L.) Heynh. Genetika 11: 51–58
Weatherwax SC, Ong MS, Degenhardt J, Bray EA, Tobin EM (1996) The interaction of light and abscisic acid in the regulation of plant gene expression. Plant Physiol 111:363–370
Wei N, Chamovitz DA, Deng X-W (1994)Arabidopsis COP9 is a component of a novel signaling complex mediating light control of development. Cell 78: 117–124
Whitelam G, Johnson E, Peng J, Carol P, Anderson ML, Cowl JS, Harberd NP (1993) Phytochrome A null mutants ofArabidopsis display a wild-type phenotype in white light. Plant Cell 5: 757–768
Whitelam GC, Miller AJ (1998) Light regulation and biological clocks,In M Anderson, JA Roberts, eds, Arabidopsis Annual Plant Reviews, Vol 1. Sheffield, England, Sheffield Academic Press, pp 331–359
Wightman F, Thimann KV (1980) Hormonal factors controlling the initiation and development of lateral roots. I. Sources of primordia-inducing substances in the primary root of pea seedlings. Physiol Plant 49: 13–20
von Arnim AG, Deng X-W (1996) Light control of seedling development. Annu Rev Plant Physiol Plant Mol Biol 47:215–244
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Soh, M.S., Hong, S.H., Kim, B.C. et al. Regulation of both light- and auxin-mediated development by theArabidopsis IAA3/SHY2 gene. J. Plant Biol. 42, 239–246 (1999). https://doi.org/10.1007/BF03030485
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DOI: https://doi.org/10.1007/BF03030485