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Influence of NaCl salinity on the behavior of hydrolases and phosphatases in mulberry genotypes: Relationship to salt tolerance

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Abstract

Two genotypes (Tr4 and Tr10) of mulberry(Morus indica) were cultured using aqueous solutions that contained different concentrations of NaCl (0 to 30 mM). Electrical conductivity (EC) values ranged from 0.52 to 4.60 mS cm-1, with a pH of 7.0. Analyses were conducted to envisage the effects of NaCl salinity on some hydrolytic enzymes. Neither nitrate reductase nor nitrite reductase activities were affected by salt treatment in roots and leaves of either genotype. The activities of β- and α-amylases were higher in leaves than in roots. Both alkaline and acid phosphatases exhibited significant increases in the roots and leaves of Tr4; no such trend was observed in Tr10. The levels of peroxidase and Superoxide dismutase increased quite significantly in the leaves as well as the roots of both genotypes. Increased enzymatic activities may suggest a stress-tolerant nature for these genotypes against NaCl toxicity. This was also evidenced by the unaffected levels in percent rooting, chloroplast pigments, osmoprotectants, nitrogenous compounds, and some biomolecules, as had been observed in earlier studies.

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Literature Cited

  • Austenfeld FA (1974) Der einfluss des NaCl and anderer alkalisakze auf die nitratreduktar aktivitat vonSalicornia europea L, 2. Pflanzenphysiol71: 288–296

    CAS  Google Scholar 

  • Beauchamp C, Fridovich I (1971) Superoxide dismutase: Improved assay and an assay applicable to acrylamide gels. Anal Biochem44: 276–287

    Article  PubMed  CAS  Google Scholar 

  • Beers RF, Sizer IW (1952) A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem195: 135–140

    Google Scholar 

  • Clairborne A (1985) Catalase activity,In RA Greenwald, ed, Handbook of Methods for Oxygen Radical Research, CRC Press, Inc, Boca Raton, Fl pp 283–284

    Google Scholar 

  • Dias MA, Costa MM (1983) Effect of low salt concentrations on nitrate reductase and peroxidase of sugar beet leaves. J Exp Bot34: 537–543

    Article  CAS  Google Scholar 

  • Dorcus D (1995) Evaluation of mulberry genotypes for drought resistance in alkaline soil. Ph.D. thesis. Bharathidasan University, Tiruchirapalli, India

    Google Scholar 

  • Dorcus D, Vivekanandan M (1991) Screening of mulberry genotypes for rainfed conditions. Sericologia31: 233–241

    Google Scholar 

  • Dubey RS, Sharma KN (1989) Acid and alkaline phosphatases in rice seedlings growing under salinity stress. Indian J Plant Physiol32: 217–223

    Google Scholar 

  • Dure LS (1960) Site of origin and extent of activity of amylases in maize germination. Plant Physiol35: 925–934

    Article  PubMed  CAS  Google Scholar 

  • Edreva AM, Georgieva ID, Cholakova NI (1989) Pathogenic and non-pathogenic stress effects on peroxidases in leaves of tobacco. Environ Exp Bot29: 365–377

    Article  CAS  Google Scholar 

  • Finka RL, Warner RL, Muzit TJ (1977) Effect of herbicides on in vivo nitrate and nitrite reduction. Weed Sci25: 18–22

    Google Scholar 

  • Franco JA, Esteber C, Rodriguez C (1993) Effects of salinity on various growth stages of muskmelon cv. Revigel. J Hort Sci68: 899–904

    CAS  Google Scholar 

  • Fridovich I (1975) Superoxide dismutase. Annu Rev Biochem4: 47–59

    Google Scholar 

  • Gnanam A, Kulandaivelu G (1969) Photosynthetic studies with leaf cell suspensions from higher plants. Plant Physiol44: 1451–1456

    Article  PubMed  CAS  Google Scholar 

  • Hageman RH, Hucklesby D (1971) Methods in Enzymology,In Sanpietro, ed, Academic Press, New York, p 491

    Google Scholar 

  • Hewitt EJ (1966) The composition of the nutrient solution,In Sand and Water Culture Methods Used in the Study of Plant Nutrition, Commonwealth Agricultural Bureaux, Farnham Royal Bucks, England, p 1990

    Google Scholar 

  • Ikawa IJ, Nisizawa K, Mira NK (1964) Specifications of several acid phosphatases from plant sources. Nature 230–239

  • Johnson DW, Smith SE, Dobrenz AK (1992) Selection for increased forage yield in alfalfa at different NaCl levels. Euphytica60: 27–35

    CAS  Google Scholar 

  • Kalir A, Poljakoff-Mayber A (1981) Changes in activity of malate dehydrogenase, catalase, peroxidase and super-oxide dismutase in leaves ofHalimore portulacoides (L.) Allen exposed to high sodium chloride concentrations. Ann Bot47: 75–85

    CAS  Google Scholar 

  • Krishna Rao R, Gnanam A (1990) Inhibition of nitrate and nitrite reductase activities by salinity stress inSorghum vulgare. Phytochemistry29: 1047–1049

    Article  Google Scholar 

  • Krishnamurthy R, Anbazhagan M, Bhagwat KA (1987) Accumulation of free amino acids and distribution of Na, Cl and K in the rice varieties exposed to NaCl stress. Indian J Plant Physiol30: 183–188

    CAS  Google Scholar 

  • Krishnaswamy S (1978) Mulberry Cultivation in South India. CSR&TI Bulletin No. 1, Mysore, India, p 15–17

    Google Scholar 

  • Lowry PH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem193: 265–275

    PubMed  CAS  Google Scholar 

  • Malik CP, Singh MB (1980) Plant Enzymology and Histoenzymology. Kalyani Publishers, New Delhi, p 53–54

    Google Scholar 

  • Mohammed Gufran Khan (1994) Effect of salt stress on nitrate reductase activity in some leguminous crops. Indian J Plant Physiol37: 185–187

    Google Scholar 

  • Paliwal K, llangovan M (1990) Factors influencing in vivo determination of nitrate reductase (EC 1.6.6.1) activity in mulberryMorus alba). Sericologia30: 369–379

    Google Scholar 

  • Penner D, Ashon FM (1967) Hormonal control of protease activity in squash cotyledons. Plant Physiol47: 791–796

    Article  Google Scholar 

  • Ramanjulu S, Veeranjaneyalu K, Sudhakar C (1993) Physiological changes induced by NaCl in mulberry var. Mysore Local. Indian J Plant Physiol36: 273–275

    Google Scholar 

  • Ramanjulu S, Veeranjaneyalu K, Sudhakar C (1994) Shortterm shifts in nitrogen metabolism in mulberryMorus alba under salt shock. Phytochemistry37: 991–995

    Article  CAS  Google Scholar 

  • Saralabai VC, Vivekanandan M (1995) Effects of the application of cement kiln exhausts on leaf biochemistry in certain legume crops. Photosynthetica31: 399–409

    CAS  Google Scholar 

  • Shaik Mohamed Anas S (1996) Effects of sodium chloride salinity on mulberry. Ph.D. thesis. Bharathidasan University, Tiruchirapalli, India.

    Google Scholar 

  • Shaik Mohamed Anas S, Vivekanandan M (1991) Are triploid mulberry varieties saline tolerant? National Sericultural Conference on Mulberry, International Sericultural Research and Training Institute, p 69

  • Shaik Mohamed Anas S, Vivekanandan M (1994a) Evaluation of salinity tolerance in mulberry varieties by exploiting rooting potential. Sericologia34: 311–321

    Google Scholar 

  • Shaik Mohamed Anas S, Vivekanandan M (1994b) Salinity responses of the triploid mulberry varieties. Indian J Serie33: 92–94

    Google Scholar 

  • Shaik Mohamed Anas S, Vivekanandan M (1999) Accumulation of certain organic metabolites in different mulberry genotypes during NaCl salinity. Sericologia39: 441–446

    Google Scholar 

  • Sreenivasulu Reddy P, Padmavathi M, Sudhakar C, Veeranjaneyalu K (1990) Photosynthetic CO2 assimilation and carbohydrate status during water stress in cowpea. Indian J Exp Biol28: 346–348

    Google Scholar 

  • Subhashini K, Reddy GM (1990) Effect of salt stress on enzyme activities in callus cultures of tolerant and susceptible rice cultivars. Indian J Exp Biol28: 277–279

    CAS  Google Scholar 

  • Susheelamma BM, Jolly, MS, Giridhar K, Sengupta K (1990) Evaluation of germplasm genotypes for drought resistance in mulberry. Sericologia30: 327–341

    Google Scholar 

  • Torriani A (1967) Alkaline phosphatases fromEscherichia coli, In GL Cantoni, eds, Procedure in Nucleic Acid Research, Harper and Row, New York

    Google Scholar 

  • Vivekanandan M, Saralabai VC (1994) SOD-An indicator of air pollution tolerance?In K Asada, T Yoshikawa, eds, Frontiers of Reactive Oxygen Species in Biology and Medicine, Excepta Medica, International Congress Series 1058, Elsevier Science BV, Amsterdam, p 553–554

    Google Scholar 

  • Zar GA (1974) Biostatistical Analysis. Prentice Hall, India

    Google Scholar 

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Authors and Affiliations

  1. International Centre for Water and Energy Systems, 2623, Abu Dhabi, United Arab Emirates

    S. Shaik Mohamed Anas

  2. Department of Biotechnology, School of Life Sciences, Bharathidasan University Tiruchirapalli, 620 024, Tamil Nadu, India

    M. Vivekanandan

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  1. S. Shaik Mohamed Anas
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  2. M. Vivekanandan
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Correspondence to M. Vivekanandan.

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Anas, S.S.M., Vivekanandan, M. Influence of NaCl salinity on the behavior of hydrolases and phosphatases in mulberry genotypes: Relationship to salt tolerance. J. Plant Biol. 43, 217–225 (2000). https://doi.org/10.1007/BF03030421

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