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Improvements of GC and HPLC analyses in solvent (acetone-butanol-ethanol) fermentation byClostridium saccharobutylicum using a mixture of starch and glycerol as carbon source

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Abstract

A study on the feasibility of using improved computer-controlled HPLC and GC systems was carried out to shorten the time needed for measuring levels of the substrates (glucose, maltose, and glycerol) and products (acetone, butanol ethanol, acetic acid, and butyric acid) produced byClostridium saccharobutylicum DSM 13864 during direct fermentation of sago starch to solvent. The use of HPLC system with a single injection to analyse the composition of culture broth (substrates and products) during solvent fermentation was achieved by raising the column temperature to 80°C. Although good separation of the components in the mixture was achieved, a slight overlap was observed in the peaks for butyric acid and acetone. The shape of the peak obtained and the analysis time of 26.66 min were satisfactory at a fixed flow rate of 0.8 mL/min. An improved GC system was developed, that was able to measure the products of solvent fermentation (acetone, butanol, ethanol, acetic acid, and butyric acid) within 19.28 min. Excellent resolution for each peak was achieved by adjusting the oven temperature to 65°C.

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Authors and Affiliations

  1. Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor D.E., Malaysia

    Liew Shiau Tsuey, Arbakariya Bin Ariff, Rosfarizan Mohamad & Raha Abdul Rahim

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  1. Liew Shiau Tsuey
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  2. Arbakariya Bin Ariff
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  3. Rosfarizan Mohamad
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  4. Raha Abdul Rahim
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Correspondence to Arbakariya Bin Ariff.

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Tsuey, L.S., Ariff, A.B., Mohamad, R. et al. Improvements of GC and HPLC analyses in solvent (acetone-butanol-ethanol) fermentation byClostridium saccharobutylicum using a mixture of starch and glycerol as carbon source. Biotechnol. Bioprocess Eng. 11, 293–298 (2006). https://doi.org/10.1007/BF03026243

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  • DOI: https://doi.org/10.1007/BF03026243

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