Abstract
The permeability of human thoracic and lumbar dura mater to various compounds in clinical use was determined in vitro. Sections of dura mater, 3 to 4 cm in diameter, obtained at post-mortem were placed between the ports (area = 3 cm2) of two glass chambers (A and B) which fitted tightly together to form a two-chamber apparatus for measuring permeability through the dura. Each chamber contained 5 ml of artificial cerebrospinal fluid. A sample of test drug solution was introduced into one chamber (A) and 50 μl aliquots were withdrawn from the other chamber (B) at predetermined intervals. Permeability was determined by calculating the rate of diffusion (slope) from the plot of mean drug concentration (chamber B) versus time. Dura mater permeability was shown to be a simple diffusion process and to be independent of lipid-solubility and molecular weight. Permeability appeared to increase with age and may have a linear relationship to the initial concentration.
Résumé
L’objectif de cette étude est d’établir in vitro la perméabilité de la dure-mère humaine à différents produits d’usage clinique courant. Des sections de dure-mère de 3 à 4 cm de diamètre obtenues à l’autopsie sont placées entre les fenêtres (aire de surface = 3 cm2) de deux chambres de verre (A et B) réunies étroitement de façon à former un appareil à deux compartiments permettant la mesure de la perméabilité dure-mèrienne. Chaque chambre contient 5 ml de liquide céphalo-rachidien artificiel. Un échantillon de la drogue-test est introduit dans la chambre A et des petites quantites de 50 μl sont retirées de l’autre chambre (B) à intervalles prédéterminés. La perméabilité est déterminée par le calcul de la vitesse de diffusion, en portant sur une courbe le tracé de la concentration moyenne de la drogue en rapport avec le temps. Les résultats montrent que la perméabilité de la dure-mère consiste en un simple processus de diffusion, independant de la solubilité lipidique et du poids moléculaire. La perméabilité semble augmenter avec l’âge et pourrait avoir une relation linéaire avec la concentration initiale.
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McEllistrem, R.E., Bennington, R.G. & Roth, S.H. In vitro determination of human dura mater permeability to opioids and local anaesthetics. Can J Anaesth 40, 165–169 (1993). https://doi.org/10.1007/BF03011315
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DOI: https://doi.org/10.1007/BF03011315